Candidatus Scalindua japonica

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Candidatus Brocadiae; Candidatus Brocadiales; Candidatus Brocadiaceae; Candidatus Scalindua

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3991 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286U0E9|A0A286U0E9_9BACT MerR-family transcriptional regulator OS=Candidatus Scalindua japonica OX=1284222 GN=SCALIN_C27_0009 PE=4 SV=1
MM1 pKa = 8.04SGMSKK6 pKa = 9.0TVVLVVARR14 pKa = 11.84CFVMVALLPCFMFSIASAQLNEE36 pKa = 4.15NCTVTVLNRR45 pKa = 11.84TVNVKK50 pKa = 10.26PDD52 pKa = 3.64GSWQLNNVPSNMGRR66 pKa = 11.84VRR68 pKa = 11.84VRR70 pKa = 11.84VTCVEE75 pKa = 4.16DD76 pKa = 3.63GKK78 pKa = 9.08TVSGQSEE85 pKa = 5.08YY86 pKa = 9.22VTIQTNGNISVEE98 pKa = 3.89EE99 pKa = 3.85MFFNNSYY106 pKa = 9.88EE107 pKa = 4.01QVPTSLTINSEE118 pKa = 3.88KK119 pKa = 10.72SVLSAIGEE127 pKa = 4.27TTRR130 pKa = 11.84ISVLAMYY137 pKa = 10.04PDD139 pKa = 4.87GSTKK143 pKa = 10.71DD144 pKa = 3.45VTLGGQGTAYY154 pKa = 9.27TVSNQAIATVNSNGLVTAISSGNIIISASNEE185 pKa = 3.94MVLSSIFLSVQLSGDD200 pKa = 3.49SDD202 pKa = 4.47GDD204 pKa = 3.79GLPDD208 pKa = 5.5DD209 pKa = 5.57FEE211 pKa = 4.74LTNGLNPNNPVDD223 pKa = 3.89SLEE226 pKa = 5.4DD227 pKa = 3.46MDD229 pKa = 6.07EE230 pKa = 4.75DD231 pKa = 4.19GLTNLEE237 pKa = 4.28EE238 pKa = 4.44FDD240 pKa = 5.45LGTGMNNADD249 pKa = 3.76TDD251 pKa = 3.88GDD253 pKa = 4.3GISDD257 pKa = 3.83GEE259 pKa = 4.33EE260 pKa = 3.57IVAGDD265 pKa = 4.2DD266 pKa = 4.22GYY268 pKa = 10.51VTNPLLGDD276 pKa = 3.69SDD278 pKa = 5.51GDD280 pKa = 4.42GIWDD284 pKa = 3.66GLEE287 pKa = 3.87VSSASDD293 pKa = 3.63PNDD296 pKa = 3.48AGSFDD301 pKa = 4.22LAATLDD307 pKa = 4.36FIKK310 pKa = 9.94VTPSNFALTFNTIMGEE326 pKa = 4.16STKK329 pKa = 10.58QLTVTGTFTDD339 pKa = 3.97GNTIDD344 pKa = 4.38LTPTGIGTNYY354 pKa = 10.0SSSDD358 pKa = 3.34LVVANFGVTAGLVYY372 pKa = 10.85AGTDD376 pKa = 3.33GTAVITVTNNGFSDD390 pKa = 3.61TAVFTVEE397 pKa = 4.43TFSPTPLSYY406 pKa = 11.42VDD408 pKa = 3.64ISGFANNVDD417 pKa = 3.32VGGDD421 pKa = 3.61YY422 pKa = 10.82AYY424 pKa = 10.93VAAGSTGLQVVDD436 pKa = 3.46VSDD439 pKa = 3.76RR440 pKa = 11.84VNPAVVASEE449 pKa = 4.18DD450 pKa = 3.73TLGNANDD457 pKa = 3.81VKK459 pKa = 11.25VDD461 pKa = 3.37GALAYY466 pKa = 9.73IADD469 pKa = 4.44GVSGLQIMDD478 pKa = 3.91ISNPLDD484 pKa = 3.74PVTVGSVDD492 pKa = 3.76TPGDD496 pKa = 3.72AYY498 pKa = 10.85DD499 pKa = 3.62VAVSGIYY506 pKa = 10.2AYY508 pKa = 10.81VADD511 pKa = 4.98GNAGLQIINVSVSNNPVIEE530 pKa = 4.35GTVVTSNIAKK540 pKa = 10.2GVDD543 pKa = 3.06IDD545 pKa = 4.17PEE547 pKa = 4.36GNLAVVADD555 pKa = 5.13GSAGVQVIDD564 pKa = 3.47ITTPSNAGIIGTVDD578 pKa = 3.1TNGNARR584 pKa = 11.84DD585 pKa = 3.67VVLRR589 pKa = 11.84NSIAYY594 pKa = 9.83VSDD597 pKa = 3.41YY598 pKa = 11.18TGGLKK603 pKa = 10.13AVNISNPTTPTIAKK617 pKa = 9.54SVEE620 pKa = 3.74GNYY623 pKa = 10.85LMDD626 pKa = 4.77AALFDD631 pKa = 4.28SLVFGADD638 pKa = 2.96VLSFNAVPAYY648 pKa = 10.3NISIPDD654 pKa = 3.81DD655 pKa = 3.74PVFTAIINFAQFRR668 pKa = 11.84DD669 pKa = 3.89DD670 pKa = 5.73DD671 pKa = 4.3GTGTDD676 pKa = 2.97ADD678 pKa = 3.64SRR680 pKa = 11.84YY681 pKa = 10.6LYY683 pKa = 9.55LTTSNGIMEE692 pKa = 4.94NGSSGDD698 pKa = 3.13TRR700 pKa = 11.84LYY702 pKa = 9.21IGQYY706 pKa = 10.95RR707 pKa = 11.84MVQDD711 pKa = 3.46IAGVPPTVSITSPLAGIDD729 pKa = 3.63VIEE732 pKa = 4.41GAKK735 pKa = 9.91IQVASDD741 pKa = 3.25AADD744 pKa = 3.82DD745 pKa = 4.01VQVASVSFIIDD756 pKa = 3.45GNVVFTDD763 pKa = 3.54TSAPYY768 pKa = 10.07EE769 pKa = 3.98FDD771 pKa = 3.32YY772 pKa = 10.73TVPLGISGVTISATALDD789 pKa = 4.14LANNAGASPDD799 pKa = 3.61LFVTVIPDD807 pKa = 3.81PLTTILGSLVDD818 pKa = 3.84INGGPMGGVTVTTVNNLSTTSLPDD842 pKa = 3.3GTFSISDD849 pKa = 3.57VPTVQGNIQVTASTNISGWPYY870 pKa = 10.27RR871 pKa = 11.84GNSPSIPAVPGGMTDD886 pKa = 2.91AGVIIIEE893 pKa = 4.11RR894 pKa = 11.84DD895 pKa = 3.67VVSITVDD902 pKa = 3.25TTIVTGNRR910 pKa = 11.84SMDD913 pKa = 4.13FKK915 pKa = 11.5ALTVDD920 pKa = 4.21GANLTVEE927 pKa = 4.8GPHH930 pKa = 5.59TFYY933 pKa = 11.09SITLLNNAKK942 pKa = 8.72LTHH945 pKa = 7.27PDD947 pKa = 2.95TTTTEE952 pKa = 4.32EE953 pKa = 4.12YY954 pKa = 10.81SLDD957 pKa = 3.25ITVSDD962 pKa = 4.2TLTIDD967 pKa = 3.1SDD969 pKa = 3.95STIDD973 pKa = 3.67VSGKK977 pKa = 10.78GYY979 pKa = 10.44LGGYY983 pKa = 8.6NGPGNYY989 pKa = 9.38SYY991 pKa = 11.51YY992 pKa = 10.98GRR994 pKa = 11.84TLGNTTTGGSYY1005 pKa = 10.2VRR1007 pKa = 11.84SGGSYY1012 pKa = 10.58GGLGGQTGSDD1022 pKa = 3.7SVNGVYY1028 pKa = 10.8GSFYY1032 pKa = 10.85NPNEE1036 pKa = 4.24LGSGGGGNSYY1046 pKa = 10.6TNGGYY1051 pKa = 10.33GGNGGGLVRR1060 pKa = 11.84ITAAEE1065 pKa = 3.77IVLNGNGSIKK1075 pKa = 10.93SNGGDD1080 pKa = 4.58GEE1082 pKa = 4.35DD1083 pKa = 4.05SDD1085 pKa = 4.55WNGGGSGGGIYY1096 pKa = 10.18INVGTIRR1103 pKa = 11.84GDD1105 pKa = 3.44GSGTIAANGGKK1116 pKa = 8.28ATRR1119 pKa = 11.84SYY1121 pKa = 11.44SSTNGGGGGRR1131 pKa = 11.84IAVYY1135 pKa = 10.54YY1136 pKa = 10.69DD1137 pKa = 4.78DD1138 pKa = 5.2IAGFDD1143 pKa = 3.62INSITAYY1150 pKa = 10.4GGLGSSNDD1158 pKa = 3.47GGAGTIYY1165 pKa = 10.74LKK1167 pKa = 10.94DD1168 pKa = 3.52VVNKK1172 pKa = 10.3KK1173 pKa = 9.12EE1174 pKa = 3.76QLIGNNSNNTGKK1186 pKa = 8.14PTPLVSEE1193 pKa = 5.07DD1194 pKa = 3.63GNGFTFDD1201 pKa = 4.42DD1202 pKa = 5.13LIIGGGSISIDD1213 pKa = 3.11KK1214 pKa = 11.02LIVNKK1219 pKa = 9.01LTLDD1223 pKa = 3.92AGDD1226 pKa = 3.54SCMGEE1231 pKa = 3.7LVVNNVVVGDD1241 pKa = 3.79GALIFAEE1248 pKa = 4.19VMTVVNNLTVDD1259 pKa = 3.37NGEE1262 pKa = 4.17LSVVDD1267 pKa = 4.68LSVGNNVTLQNNGILTHH1284 pKa = 6.56EE1285 pKa = 4.67AATMTQAYY1293 pKa = 9.7SSTIGVGGTLAIDD1306 pKa = 3.49SGSTIDD1312 pKa = 3.99VSCKK1316 pKa = 10.49GYY1318 pKa = 10.5LGGYY1322 pKa = 8.56NGSGNYY1328 pKa = 9.67SYY1330 pKa = 11.48YY1331 pKa = 10.98GRR1333 pKa = 11.84TLGNTTTGGSYY1344 pKa = 10.2VRR1346 pKa = 11.84SGGSYY1351 pKa = 10.58GGLGGQTGTDD1361 pKa = 3.38NVNGVYY1367 pKa = 10.75GSFYY1371 pKa = 10.85NPNEE1375 pKa = 4.24LGSGGGGNSYY1385 pKa = 10.6TNGGYY1390 pKa = 10.33GGNGGGLVRR1399 pKa = 11.84ITAAEE1404 pKa = 3.77IVLNGNGSIKK1414 pKa = 10.93SNGGDD1419 pKa = 4.58GEE1421 pKa = 4.35DD1422 pKa = 4.05SDD1424 pKa = 4.55WNGGGSGGGIYY1435 pKa = 10.18INVGTIRR1442 pKa = 11.84GDD1444 pKa = 3.44GSGTIAANGGKK1455 pKa = 8.28ATRR1458 pKa = 11.84SYY1460 pKa = 11.44SSTNGGGGGRR1470 pKa = 11.84IAVYY1474 pKa = 10.54YY1475 pKa = 10.69DD1476 pKa = 4.78DD1477 pKa = 5.2IAGFDD1482 pKa = 3.62INSITAYY1489 pKa = 10.4GGLGSSNDD1497 pKa = 3.47GGAGTIYY1504 pKa = 10.57LKK1506 pKa = 11.0ASIQTYY1512 pKa = 10.8GDD1514 pKa = 4.41LIVDD1518 pKa = 4.1NNGSTSLVYY1527 pKa = 9.12STPLVSVGTGLSSDD1541 pKa = 3.63LTANTLTDD1549 pKa = 3.55STRR1552 pKa = 11.84KK1553 pKa = 9.22WNVDD1557 pKa = 2.94GLIGIFLNPNTSQEE1571 pKa = 4.28TTPATVFRR1579 pKa = 11.84ILSNDD1584 pKa = 2.99VTSITVEE1591 pKa = 3.79NTLNNLTDD1599 pKa = 3.43VTISGNSYY1607 pKa = 10.01IGEE1610 pKa = 4.33HH1611 pKa = 6.17YY1612 pKa = 10.4FDD1614 pKa = 4.85NLTIINGARR1623 pKa = 11.84VEE1625 pKa = 4.22TLDD1628 pKa = 4.71RR1629 pKa = 11.84ISFSYY1634 pKa = 10.72ILITSGGEE1642 pKa = 3.81IQADD1646 pKa = 3.38NYY1648 pKa = 9.42YY1649 pKa = 10.61QIGKK1653 pKa = 10.19AEE1655 pKa = 4.1TSLKK1659 pKa = 10.88DD1660 pKa = 4.08KK1661 pKa = 10.29LAKK1664 pKa = 9.17YY1665 pKa = 9.25QKK1667 pKa = 10.42KK1668 pKa = 10.01

Molecular weight: 170.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286U2R1|A0A286U2R1_9BACT Formylglycine-generating sulfatase-like enzyme OS=Candidatus Scalindua japonica OX=1284222 GN=SCALIN_C31_0051 PE=4 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.57LKK5 pKa = 8.67THH7 pKa = 6.6KK8 pKa = 10.66GLTKK12 pKa = 10.07RR13 pKa = 11.84VKK15 pKa = 10.11VSPNGKK21 pKa = 8.42IKK23 pKa = 10.24RR24 pKa = 11.84RR25 pKa = 11.84KK26 pKa = 9.38AFAGHH31 pKa = 6.93LMSGKK36 pKa = 8.3TGNRR40 pKa = 11.84KK41 pKa = 9.04RR42 pKa = 11.84ALKK45 pKa = 9.31RR46 pKa = 11.84TSLVSKK52 pKa = 10.16GFIRR56 pKa = 11.84PLMRR60 pKa = 11.84ALGKK64 pKa = 10.33AA65 pKa = 3.3

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3991

0

3991

1248076

33

4670

312.7

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.136 ± 0.033

1.265 ± 0.016

5.77 ± 0.029

7.127 ± 0.032

4.345 ± 0.027

6.833 ± 0.041

2.02 ± 0.019

8.21 ± 0.039

7.826 ± 0.041

9.081 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.395 ± 0.017

5.012 ± 0.027

3.588 ± 0.022

2.955 ± 0.019

4.618 ± 0.03

6.49 ± 0.031

5.247 ± 0.032

6.604 ± 0.033

1.025 ± 0.013

3.455 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski