Orbus hercynius

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Orbales; Orbaceae; Orbus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495RFP5|A0A495RFP5_9GAMM Outer membrane scaffolding protein for murein synthesis (MipA/OmpV family) OS=Orbus hercynius OX=593135 GN=DES39_1455 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 5.52FDD4 pKa = 4.31TSVLCDD10 pKa = 5.21FYY12 pKa = 11.01PEE14 pKa = 5.29DD15 pKa = 3.93INVVEE20 pKa = 5.19PIFTNFGGISQFSGQVVTVKK40 pKa = 10.56CFEE43 pKa = 4.93DD44 pKa = 3.39NGLLYY49 pKa = 10.44EE50 pKa = 4.2VLQNDD55 pKa = 4.0GTGKK59 pKa = 10.32VLVVDD64 pKa = 4.19GGGSVRR70 pKa = 11.84RR71 pKa = 11.84ALLDD75 pKa = 3.68GEE77 pKa = 4.61LVDD80 pKa = 5.37IAVQNSWQGIIIYY93 pKa = 9.87GAVRR97 pKa = 11.84QVDD100 pKa = 3.83YY101 pKa = 11.12LADD104 pKa = 3.77AEE106 pKa = 4.43IGIQATAAIPVGAEE120 pKa = 3.7DD121 pKa = 3.71QGIGEE126 pKa = 4.48TDD128 pKa = 2.87IKK130 pKa = 11.51VNFAGVTFFSGDD142 pKa = 3.81FIYY145 pKa = 10.94ADD147 pKa = 3.31NTGIILSEE155 pKa = 3.62IALEE159 pKa = 4.09IEE161 pKa = 4.4EE162 pKa = 4.27

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495RI46|A0A495RI46_9GAMM 3-keto-L-gulonate 6-phosphate decarboxylase OS=Orbus hercynius OX=593135 GN=DES39_0422 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.68RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.28GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.44GRR39 pKa = 11.84VRR41 pKa = 11.84LTVSSSS47 pKa = 2.75

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2085

0

2085

690237

29

6000

331.0

36.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.068 ± 0.048

1.104 ± 0.018

5.446 ± 0.042

5.201 ± 0.049

4.175 ± 0.039

6.385 ± 0.059

2.23 ± 0.025

8.056 ± 0.049

5.756 ± 0.045

10.544 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.026

4.97 ± 0.05

3.736 ± 0.031

4.792 ± 0.045

4.108 ± 0.041

6.331 ± 0.048

5.475 ± 0.039

6.467 ± 0.042

1.117 ± 0.02

3.544 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski