Synechococcus phage B3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 370 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650F9M7|A0A650F9M7_9CAUD Uncharacterized protein OS=Synechococcus phage B3 OX=2674978 GN=b3_0118 PE=4 SV=1
MM1 pKa = 7.3SVIVYY6 pKa = 9.72LLSQNPMIKK15 pKa = 9.35TKK17 pKa = 10.93ADD19 pKa = 3.98LIEE22 pKa = 4.29FLEE25 pKa = 4.71NYY27 pKa = 9.46PDD29 pKa = 5.07DD30 pKa = 3.5IRR32 pKa = 11.84ITVGPCSDD40 pKa = 4.35DD41 pKa = 4.14LVISEE46 pKa = 4.96CIGYY50 pKa = 10.34AGITLNFSGDD60 pKa = 3.56FGDD63 pKa = 5.09DD64 pKa = 3.11

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650EY09|A0A650EY09_9CAUD Uncharacterized protein OS=Synechococcus phage B3 OX=2674978 GN=b3_0256 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84APNINPMNTKK12 pKa = 8.42ITGQDD17 pKa = 3.69LINYY21 pKa = 7.95RR22 pKa = 11.84EE23 pKa = 3.87HH24 pKa = 6.81WKK26 pKa = 10.45AQGATNADD34 pKa = 3.36IVRR37 pKa = 11.84GAGYY41 pKa = 8.36ITIRR45 pKa = 11.84KK46 pKa = 9.89DD47 pKa = 2.96GTQRR51 pKa = 11.84LNFTEE56 pKa = 5.08FYY58 pKa = 10.67EE59 pKa = 4.56NILIARR65 pKa = 11.84GRR67 pKa = 11.84MWRR70 pKa = 11.84ITVNVAEE77 pKa = 4.4NTSAGAQPIWSDD89 pKa = 3.18SFTTSSRR96 pKa = 11.84SARR99 pKa = 11.84SVAHH103 pKa = 6.76KK104 pKa = 10.67VRR106 pKa = 11.84ALTHH110 pKa = 5.17LTGARR115 pKa = 11.84CKK117 pKa = 9.94RR118 pKa = 11.84VVEE121 pKa = 4.07NGVVKK126 pKa = 10.31LYY128 pKa = 10.09PYY130 pKa = 10.47RR131 pKa = 11.84SNQMVAYY138 pKa = 9.37NLPAA142 pKa = 5.47

Molecular weight: 16.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

370

0

370

74848

30

2845

202.3

22.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.436 ± 0.364

1.243 ± 0.085

6.012 ± 0.107

6.624 ± 0.227

4.43 ± 0.105

6.242 ± 0.255

1.709 ± 0.099

7.939 ± 0.216

6.814 ± 0.286

7.933 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.028 ± 0.135

6.569 ± 0.193

3.836 ± 0.108

3.374 ± 0.1

4.025 ± 0.119

7.08 ± 0.216

6.422 ± 0.266

6.128 ± 0.149

1.21 ± 0.087

4.905 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski