Subterranean clover stunt virus (strain F) (SCSV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Nanovirus; Subterranean clover stunt virus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q87011|NSP_SCSVF Putative nuclear shuttle protein OS=Subterranean clover stunt virus (strain F) OX=291607 GN=DNA-N PE=3 SV=1
MM1 pKa = 7.48ALRR4 pKa = 11.84YY5 pKa = 8.98FSHH8 pKa = 7.56LPEE11 pKa = 4.22EE12 pKa = 4.36LKK14 pKa = 10.74EE15 pKa = 4.32KK16 pKa = 10.58IMNEE20 pKa = 3.59HH21 pKa = 6.31LKK23 pKa = 10.32EE24 pKa = 4.04IKK26 pKa = 10.25KK27 pKa = 10.55KK28 pKa = 10.27EE29 pKa = 3.8FLEE32 pKa = 4.11NVIKK36 pKa = 10.4AACAVFEE43 pKa = 4.22GLTKK47 pKa = 10.36KK48 pKa = 10.74EE49 pKa = 4.25SVEE52 pKa = 3.74EE53 pKa = 3.91DD54 pKa = 3.73DD55 pKa = 4.07ILRR58 pKa = 11.84FSGFLEE64 pKa = 4.41GLSAYY69 pKa = 9.89YY70 pKa = 10.87AEE72 pKa = 4.31ATKK75 pKa = 10.49KK76 pKa = 10.24KK77 pKa = 10.69CLVRR81 pKa = 11.84WKK83 pKa = 10.58KK84 pKa = 10.72SVAINLKK91 pKa = 7.71WRR93 pKa = 11.84VMEE96 pKa = 4.0EE97 pKa = 3.64MHH99 pKa = 6.26YY100 pKa = 10.89KK101 pKa = 10.76LYY103 pKa = 11.14GFADD107 pKa = 4.04MEE109 pKa = 4.39DD110 pKa = 4.86LYY112 pKa = 11.24CSEE115 pKa = 5.53LGFPNYY121 pKa = 10.8GEE123 pKa = 5.39DD124 pKa = 3.91DD125 pKa = 3.72VAYY128 pKa = 10.4HH129 pKa = 7.32DD130 pKa = 4.47GAIVNCKK137 pKa = 8.04QLEE140 pKa = 4.44VVFDD144 pKa = 4.11DD145 pKa = 4.95LGIEE149 pKa = 4.24FMSIVIDD156 pKa = 3.64RR157 pKa = 11.84GSIKK161 pKa = 10.15IEE163 pKa = 3.88LL164 pKa = 3.92

Molecular weight: 19.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q87013|REP6_SCSVF Para-Rep C6 OS=Subterranean clover stunt virus (strain F) OX=291607 GN=C6 PE=3 SV=1
MM1 pKa = 7.21VAVRR5 pKa = 11.84WGRR8 pKa = 11.84KK9 pKa = 6.69GLRR12 pKa = 11.84SQRR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 9.78YY18 pKa = 10.97SRR20 pKa = 11.84IAYY23 pKa = 7.64KK24 pKa = 10.32PPSSKK29 pKa = 10.36VVSHH33 pKa = 6.27VEE35 pKa = 3.85SVLNKK40 pKa = 9.98RR41 pKa = 11.84DD42 pKa = 3.47VTGAEE47 pKa = 4.03VKK49 pKa = 10.44PFADD53 pKa = 4.2GSRR56 pKa = 11.84YY57 pKa = 9.71SMKK60 pKa = 10.53KK61 pKa = 9.85VMLIATLTMAPGEE74 pKa = 4.11LVNYY78 pKa = 9.44LIVKK82 pKa = 9.7SNSPIANWSSSFSNPSLMVKK102 pKa = 10.42EE103 pKa = 4.62SVQDD107 pKa = 3.33TVTIVGGGKK116 pKa = 9.93LEE118 pKa = 4.2SSGTAGKK125 pKa = 10.22DD126 pKa = 3.12VTKK129 pKa = 10.66SFRR132 pKa = 11.84KK133 pKa = 9.15FVKK136 pKa = 9.96LGSGISQTQHH146 pKa = 6.55LYY148 pKa = 11.09LIIYY152 pKa = 10.02SSDD155 pKa = 2.77AMKK158 pKa = 9.31ITLEE162 pKa = 3.66TRR164 pKa = 11.84MYY166 pKa = 10.75IDD168 pKa = 3.15VV169 pKa = 3.93

Molecular weight: 18.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1595

112

286

199.4

23.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.953 ± 0.566

2.571 ± 0.417

5.643 ± 0.653

8.025 ± 0.993

4.075 ± 0.437

6.27 ± 0.335

1.944 ± 0.338

5.831 ± 0.331

7.649 ± 0.545

7.524 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.574 ± 0.137

3.824 ± 0.367

3.197 ± 0.527

3.26 ± 0.48

6.27 ± 0.482

6.959 ± 0.871

4.013 ± 0.471

7.774 ± 0.79

1.818 ± 0.25

4.828 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski