Thermofilum adornatum

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Thermofilales; Thermofilaceae;

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1896 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5ZD19|S5ZD19_9CREN M20_dimer domain-containing protein OS=Thermofilum adornatum OX=1365176 GN=N186_02585 PE=4 SV=1
MM1 pKa = 7.68CIVFLLAFDD10 pKa = 5.4DD11 pKa = 4.78EE12 pKa = 4.66FALDD16 pKa = 3.74VFGHH20 pKa = 5.66FLIGNMWQSVRR31 pKa = 11.84YY32 pKa = 9.57GLGEE36 pKa = 4.04DD37 pKa = 3.56VAVFLFLLEE46 pKa = 4.13

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5ZDL6|S5ZDL6_9CREN Uncharacterized protein OS=Thermofilum adornatum OX=1365176 GN=N186_03655 PE=4 SV=1
MM1 pKa = 8.72VEE3 pKa = 3.99MAHH6 pKa = 6.66FKK8 pKa = 10.65PLGKK12 pKa = 10.13KK13 pKa = 9.77LRR15 pKa = 11.84LAAATRR21 pKa = 11.84SNQQIPIWIIGKK33 pKa = 7.27TLGKK37 pKa = 10.03VRR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 9.44PRR43 pKa = 11.84RR44 pKa = 11.84NWRR47 pKa = 11.84RR48 pKa = 11.84SRR50 pKa = 11.84MQLL53 pKa = 3.09

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1896

0

1896

523380

29

1714

276.0

30.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.326 ± 0.054

0.686 ± 0.019

4.337 ± 0.037

7.398 ± 0.073

4.034 ± 0.039

6.972 ± 0.057

1.496 ± 0.023

7.266 ± 0.049

6.479 ± 0.054

11.298 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.077 ± 0.026

3.206 ± 0.04

4.567 ± 0.038

2.521 ± 0.033

5.652 ± 0.054

6.315 ± 0.053

4.985 ± 0.053

8.216 ± 0.055

1.174 ± 0.023

3.996 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski