Magnetospirillum moscoviense
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3747 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A178MPG4|A0A178MPG4_9PROT Phosphopantetheine adenylyltransferase OS=Magnetospirillum moscoviense OX=1437059 GN=coaD PE=3 SV=1
MM1 pKa = 7.87 AYY3 pKa = 10.3 LSKK6 pKa = 10.67 NLSVLSSANAFTMWHH21 pKa = 5.43 YY22 pKa = 7.47 TTTDD26 pKa = 2.81 TAATVDD32 pKa = 3.4 TEE34 pKa = 5.26 GYY36 pKa = 9.58 FNQASGMLRR45 pKa = 11.84 VGDD48 pKa = 4.34 FIVANVDD55 pKa = 3.15 TDD57 pKa = 3.55 GTPAHH62 pKa = 6.44 GLFVVLSNAAGVVDD76 pKa = 4.27 VGDD79 pKa = 3.86 MVAVGGTDD87 pKa = 2.97 TDD89 pKa = 3.51
Molecular weight: 9.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.821
IPC_protein 3.757
Toseland 3.528
ProMoST 3.872
Dawson 3.795
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.592
Grimsley 3.452
Solomon 3.77
Lehninger 3.719
Nozaki 3.935
DTASelect 4.24
Thurlkill 3.63
EMBOSS 3.808
Sillero 3.897
Patrickios 1.926
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.864
Protein with the highest isoelectric point:
>tr|A0A178MGP8|A0A178MGP8_9PROT DUF218 domain-containing protein OS=Magnetospirillum moscoviense OX=1437059 GN=A6A05_03220 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 8.7 IVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.66 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.32 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.46 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3747
0
3747
1212731
41
14963
323.7
35.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.842 ± 0.069
0.963 ± 0.017
6.001 ± 0.036
5.507 ± 0.048
3.472 ± 0.024
8.611 ± 0.051
2.185 ± 0.026
4.849 ± 0.032
3.489 ± 0.042
10.373 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.572 ± 0.025
2.454 ± 0.043
5.126 ± 0.048
3.216 ± 0.023
6.945 ± 0.075
5.07 ± 0.057
5.229 ± 0.1
7.732 ± 0.032
1.367 ± 0.016
1.996 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here