Magnetospirillum moscoviense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Magnetospirillum

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A178MPG4|A0A178MPG4_9PROT Phosphopantetheine adenylyltransferase OS=Magnetospirillum moscoviense OX=1437059 GN=coaD PE=3 SV=1
MM1 pKa = 7.87AYY3 pKa = 10.3LSKK6 pKa = 10.67NLSVLSSANAFTMWHH21 pKa = 5.43YY22 pKa = 7.47TTTDD26 pKa = 2.81TAATVDD32 pKa = 3.4TEE34 pKa = 5.26GYY36 pKa = 9.58FNQASGMLRR45 pKa = 11.84VGDD48 pKa = 4.34FIVANVDD55 pKa = 3.15TDD57 pKa = 3.55GTPAHH62 pKa = 6.44GLFVVLSNAAGVVDD76 pKa = 4.27VGDD79 pKa = 3.86MVAVGGTDD87 pKa = 2.97TDD89 pKa = 3.51

Molecular weight:
9.24 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A178MGP8|A0A178MGP8_9PROT DUF218 domain-containing protein OS=Magnetospirillum moscoviense OX=1437059 GN=A6A05_03220 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 8.7IVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.66GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.32VIANRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.46GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.11 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3747

0

3747

1212731

41

14963

323.7

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.842 ± 0.069

0.963 ± 0.017

6.001 ± 0.036

5.507 ± 0.048

3.472 ± 0.024

8.611 ± 0.051

2.185 ± 0.026

4.849 ± 0.032

3.489 ± 0.042

10.373 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.572 ± 0.025

2.454 ± 0.043

5.126 ± 0.048

3.216 ± 0.023

6.945 ± 0.075

5.07 ± 0.057

5.229 ± 0.1

7.732 ± 0.032

1.367 ± 0.016

1.996 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski