Meles meles circovirus-like virus
Average proteome isoelectric point is 7.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9LQV7|G9LQV7_9CIRC Capsid protein OS=Meles meles circovirus-like virus OX=1128073 PE=4 SV=1
MM1 pKa = 7.24 ATRR4 pKa = 11.84 PEE6 pKa = 3.99 ALHH9 pKa = 6.26 WCFTLNNPTIDD20 pKa = 3.53 EE21 pKa = 4.03 QQIIDD26 pKa = 3.84 EE27 pKa = 4.48 LEE29 pKa = 3.94 EE30 pKa = 3.99 FGIEE34 pKa = 3.99 YY35 pKa = 10.58 AVFQLEE41 pKa = 4.15 EE42 pKa = 4.56 GEE44 pKa = 4.35 NNTPHH49 pKa = 5.72 FQGYY53 pKa = 8.35 VALTVKK59 pKa = 10.31 KK60 pKa = 10.19 RR61 pKa = 11.84 LTAVRR66 pKa = 11.84 TLFGGVAHH74 pKa = 6.82 WEE76 pKa = 4.18 ICRR79 pKa = 11.84 GTPTQNRR86 pKa = 11.84 DD87 pKa = 3.08 YY88 pKa = 9.93 CTKK91 pKa = 10.43 ADD93 pKa = 3.59 TRR95 pKa = 11.84 IGDD98 pKa = 3.7 FVEE101 pKa = 4.93 IGTLPAPKK109 pKa = 10.04 GARR112 pKa = 11.84 QDD114 pKa = 3.96 LVQLHH119 pKa = 6.25 SALKK123 pKa = 10.71 DD124 pKa = 3.31 GLTQAQYY131 pKa = 11.02 VDD133 pKa = 3.44 QFFSIFVRR141 pKa = 11.84 HH142 pKa = 6.35 PNLVSNYY149 pKa = 7.54 VAATISEE156 pKa = 4.33 RR157 pKa = 11.84 DD158 pKa = 3.27 PANPVSTWLLIGTPGTGKK176 pKa = 10.26 SRR178 pKa = 11.84 LARR181 pKa = 11.84 AIGKK185 pKa = 8.76 NLGDD189 pKa = 3.56 GAIFEE194 pKa = 4.64 HH195 pKa = 7.27 SLGKK199 pKa = 9.81 WFDD202 pKa = 3.74 GYY204 pKa = 11.03 VGQRR208 pKa = 11.84 TVLFDD213 pKa = 4.08 DD214 pKa = 4.8 FCGSSLPFSQFKK226 pKa = 10.17 RR227 pKa = 11.84 ICDD230 pKa = 3.78 RR231 pKa = 11.84 YY232 pKa = 8.76 PVRR235 pKa = 11.84 VEE237 pKa = 4.87 LKK239 pKa = 10.74 GLSCPMAATNFLITTNQDD257 pKa = 2.68 PKK259 pKa = 10.37 TWWKK263 pKa = 11.3 DD264 pKa = 3.42 EE265 pKa = 3.83 VTGQHH270 pKa = 4.7 GHH272 pKa = 5.92 AAITRR277 pKa = 11.84 RR278 pKa = 11.84 IGRR281 pKa = 11.84 VLFFFAEE288 pKa = 4.24 NTFRR292 pKa = 11.84 SYY294 pKa = 11.23 PSYY297 pKa = 10.87 NHH299 pKa = 6.47 YY300 pKa = 9.95 ATDD303 pKa = 3.84 CLRR306 pKa = 11.84 PRR308 pKa = 11.84 IDD310 pKa = 3.42 GEE312 pKa = 4.45 TYY314 pKa = 10.11 HH315 pKa = 6.35 YY316 pKa = 9.82 FSPAQTIVYY325 pKa = 8.47 DD326 pKa = 4.01 AQGEE330 pKa = 4.47 AILPPEE336 pKa = 4.14 ILRR339 pKa = 11.84 AEE341 pKa = 4.38 VQQ343 pKa = 3.11
Molecular weight: 38.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.747
IPC2_protein 5.741
IPC_protein 5.792
Toseland 6.173
ProMoST 6.135
Dawson 6.071
Bjellqvist 6.071
Wikipedia 6.084
Rodwell 6.059
Grimsley 6.313
Solomon 6.071
Lehninger 6.071
Nozaki 6.338
DTASelect 6.532
Thurlkill 6.547
EMBOSS 6.504
Sillero 6.44
Patrickios 3.973
IPC_peptide 6.084
IPC2_peptide 6.44
IPC2.peptide.svr19 6.381
Protein with the highest isoelectric point:
>tr|G9LQV7|G9LQV7_9CIRC Capsid protein OS=Meles meles circovirus-like virus OX=1128073 PE=4 SV=1
MM1 pKa = 7.47 GRR3 pKa = 11.84 RR4 pKa = 11.84 ITTFRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SSTMRR18 pKa = 11.84 KK19 pKa = 9.1 ARR21 pKa = 11.84 PSYY24 pKa = 10.06 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 SSARR31 pKa = 11.84 KK32 pKa = 7.87 YY33 pKa = 9.04 SKK35 pKa = 10.13 KK36 pKa = 9.68 RR37 pKa = 11.84 RR38 pKa = 11.84 SRR40 pKa = 11.84 GMQRR44 pKa = 11.84 IKK46 pKa = 9.78 TSRR49 pKa = 11.84 WPVRR53 pKa = 11.84 NIGGDD58 pKa = 3.11 RR59 pKa = 11.84 SFCKK63 pKa = 10.12 LRR65 pKa = 11.84 YY66 pKa = 9.02 VKK68 pKa = 10.66 GSAFDD73 pKa = 3.58 IPEE76 pKa = 4.34 GAASLSQLQVMNVGAYY92 pKa = 9.27 PGASNLLQSMTLNGVMGEE110 pKa = 4.3 TPNLSTMGALYY121 pKa = 9.48 TRR123 pKa = 11.84 YY124 pKa = 9.87 RR125 pKa = 11.84 IRR127 pKa = 11.84 GIKK130 pKa = 9.28 IRR132 pKa = 11.84 LTYY135 pKa = 8.28 WQLGGAPVILFTNAAPDD152 pKa = 3.82 VNNLTVASTGPNPAFITPNITTTTEE177 pKa = 3.89 QRR179 pKa = 11.84 WAKK182 pKa = 10.51 YY183 pKa = 9.36 RR184 pKa = 11.84 VCQATANGGKK194 pKa = 8.19 ATSVSAYY201 pKa = 9.28 YY202 pKa = 10.34 SVNRR206 pKa = 11.84 VQGPDD211 pKa = 3.27 TIVRR215 pKa = 11.84 NDD217 pKa = 3.42 RR218 pKa = 11.84 DD219 pKa = 3.93 YY220 pKa = 11.31 TGEE223 pKa = 4.03 MQPATPYY230 pKa = 9.53 FANSSGVADD239 pKa = 4.39 TPQFSPWMQWGIATLSGTPAVAGTQGVIKK268 pKa = 10.64 VEE270 pKa = 3.8 QTVYY274 pKa = 11.2 CEE276 pKa = 4.08 FFGKK280 pKa = 10.49 RR281 pKa = 11.84 IQTQQ285 pKa = 2.83
Molecular weight: 31.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 10.175
IPC_protein 11.155
Toseland 11.111
ProMoST 11.301
Dawson 11.184
Bjellqvist 11.038
Wikipedia 11.535
Rodwell 11.184
Grimsley 11.242
Solomon 11.477
Lehninger 11.403
Nozaki 11.096
DTASelect 11.038
Thurlkill 11.111
EMBOSS 11.564
Sillero 11.14
Patrickios 10.862
IPC_peptide 11.477
IPC2_peptide 10.365
IPC2.peptide.svr19 8.421
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
628
285
343
314.0
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.121 ± 0.182
1.592 ± 0.328
4.14 ± 1.023
4.14 ± 1.236
4.777 ± 0.983
8.121 ± 0.395
1.592 ± 0.967
5.414 ± 0.305
3.822 ± 0.236
6.369 ± 1.098
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.752 ± 0.854
4.299 ± 0.372
5.573 ± 0.025
5.096 ± 0.102
8.439 ± 1.48
5.892 ± 1.323
8.917 ± 0.551
6.051 ± 0.161
1.752 ± 0.002
4.14 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here