Pseudozobellia thermophila

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Pseudozobellia

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6KCS0|A0A1M6KCS0_9FLAO Predicted dehydrogenase OS=Pseudozobellia thermophila OX=192903 GN=SAMN04488513_10614 PE=4 SV=1
MM1 pKa = 7.31NRR3 pKa = 11.84TLSHH7 pKa = 6.16LQVLSLTFLSLLTFSSCSSDD27 pKa = 5.3DD28 pKa = 4.94DD29 pKa = 5.64DD30 pKa = 6.74DD31 pKa = 5.73NLGNWVDD38 pKa = 3.5RR39 pKa = 11.84SVFDD43 pKa = 3.56GSPRR47 pKa = 11.84SSVSGFTIGDD57 pKa = 3.51VGYY60 pKa = 9.62MGLGYY65 pKa = 10.86DD66 pKa = 4.13GDD68 pKa = 5.0DD69 pKa = 3.73YY70 pKa = 11.97LNSFWSYY77 pKa = 11.58DD78 pKa = 3.46LQGDD82 pKa = 3.99YY83 pKa = 10.26WSQKK87 pKa = 10.71ADD89 pKa = 3.81FPGSARR95 pKa = 11.84NAAVGFEE102 pKa = 4.05IDD104 pKa = 3.2GTGYY108 pKa = 10.72LGSGYY113 pKa = 10.57DD114 pKa = 4.02GLDD117 pKa = 3.5EE118 pKa = 5.97LSDD121 pKa = 4.15FYY123 pKa = 11.58AYY125 pKa = 10.37DD126 pKa = 3.87PSTNTWTEE134 pKa = 3.67IAPTPAAARR143 pKa = 11.84RR144 pKa = 11.84SAVAFGANGYY154 pKa = 10.42GYY156 pKa = 10.53FGTGYY161 pKa = 10.63DD162 pKa = 4.01GEE164 pKa = 4.31NDD166 pKa = 4.08RR167 pKa = 11.84KK168 pKa = 10.83DD169 pKa = 3.15FWKK172 pKa = 10.68YY173 pKa = 11.09DD174 pKa = 3.58PSTDD178 pKa = 3.01TWSEE182 pKa = 3.81LVGFGGDD189 pKa = 3.26KK190 pKa = 10.73RR191 pKa = 11.84RR192 pKa = 11.84DD193 pKa = 3.41AVTFTIDD200 pKa = 3.09NTVYY204 pKa = 10.97LGTGVSNGIYY214 pKa = 10.32LDD216 pKa = 4.59DD217 pKa = 4.7FWAFDD222 pKa = 4.15PSTEE226 pKa = 3.81TWTKK230 pKa = 11.04KK231 pKa = 10.49LDD233 pKa = 3.73LDD235 pKa = 4.33EE236 pKa = 5.32EE237 pKa = 4.85DD238 pKa = 4.83DD239 pKa = 4.03YY240 pKa = 12.08SIVRR244 pKa = 11.84SNAVAFTLNGYY255 pKa = 10.18GYY257 pKa = 9.5IACGYY262 pKa = 8.05ATGALGTVWEE272 pKa = 5.02YY273 pKa = 11.08IPGTDD278 pKa = 2.68SWEE281 pKa = 4.02AKK283 pKa = 9.58TSFEE287 pKa = 3.81GTTRR291 pKa = 11.84QDD293 pKa = 3.78AIAFSNGTRR302 pKa = 11.84AFVGLGRR309 pKa = 11.84SGTLYY314 pKa = 10.91LDD316 pKa = 4.55DD317 pKa = 5.55LDD319 pKa = 4.41EE320 pKa = 5.01FFPFDD325 pKa = 4.14EE326 pKa = 5.11YY327 pKa = 11.59DD328 pKa = 4.75DD329 pKa = 4.28EE330 pKa = 4.99DD331 pKa = 3.69

Molecular weight: 36.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6PAH8|A0A1M6PAH8_9FLAO Predicted naringenin-chalcone synthase OS=Pseudozobellia thermophila OX=192903 GN=SAMN04488513_11917 PE=3 SV=1
MM1 pKa = 7.69AEE3 pKa = 3.74RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.21MVRR9 pKa = 11.84KK10 pKa = 9.19GHH12 pKa = 6.0SKK14 pKa = 10.83LSISAQCRR22 pKa = 11.84SLGIHH27 pKa = 6.96RR28 pKa = 11.84SGLYY32 pKa = 9.02YY33 pKa = 10.28RR34 pKa = 11.84PRR36 pKa = 11.84GEE38 pKa = 4.12TEE40 pKa = 4.08LNLEE44 pKa = 4.44LMRR47 pKa = 11.84LMDD50 pKa = 3.57EE51 pKa = 4.65HH52 pKa = 8.1YY53 pKa = 10.34LYY55 pKa = 10.87HH56 pKa = 6.85PEE58 pKa = 3.96KK59 pKa = 10.0GAKK62 pKa = 9.27RR63 pKa = 11.84MHH65 pKa = 5.54VWLTRR70 pKa = 11.84DD71 pKa = 2.72KK72 pKa = 10.91GYY74 pKa = 10.65KK75 pKa = 9.03VSRR78 pKa = 11.84NRR80 pKa = 11.84II81 pKa = 3.5

Molecular weight: 9.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4169

0

4169

1506813

39

4873

361.4

40.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.957 ± 0.033

0.743 ± 0.012

6.036 ± 0.034

6.713 ± 0.03

4.902 ± 0.027

7.3 ± 0.033

1.919 ± 0.017

6.747 ± 0.031

6.904 ± 0.044

9.386 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.017

5.21 ± 0.03

3.892 ± 0.022

3.275 ± 0.016

4.157 ± 0.025

6.158 ± 0.03

5.583 ± 0.038

6.527 ± 0.03

1.211 ± 0.015

4.109 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski