Giant panda polyomavirus
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IG73|A0A220IG73_9POLY Minor capsid protein OS=Giant panda polyomavirus OX=2016463 PE=3 SV=1
MM1 pKa = 7.47 GGVLSIVVDD10 pKa = 4.58 LFTLLPEE17 pKa = 4.81 LSASTGFGIEE27 pKa = 4.44 AILAGEE33 pKa = 4.15 AAASVEE39 pKa = 4.07 AQVASLMLIEE49 pKa = 4.55 TMGPLDD55 pKa = 4.02 ALASLGLSAEE65 pKa = 4.38 TFSLLSAMPGFFQDD79 pKa = 3.17 IVGLGVLFQTVSGASSLVAAGIQLGRR105 pKa = 11.84 HH106 pKa = 4.89 EE107 pKa = 4.42 VSVVNRR113 pKa = 11.84 NMALVPWIPQDD124 pKa = 3.57 LFDD127 pKa = 4.7 IYY129 pKa = 11.02 FPGVSSFSYY138 pKa = 10.26 VVNIVADD145 pKa = 3.38 WGEE148 pKa = 4.32 SLFHH152 pKa = 6.61 SLARR156 pKa = 11.84 NIWDD160 pKa = 3.25 ALMRR164 pKa = 11.84 EE165 pKa = 4.09 SRR167 pKa = 11.84 AQITHH172 pKa = 6.17 ATRR175 pKa = 11.84 EE176 pKa = 4.05 LTLRR180 pKa = 11.84 GVQTFQDD187 pKa = 3.04 TMARR191 pKa = 11.84 VIEE194 pKa = 3.99 RR195 pKa = 11.84 ARR197 pKa = 11.84 WVVTNGPVHH206 pKa = 7.02 AYY208 pKa = 10.0 SAIEE212 pKa = 4.08 EE213 pKa = 4.67 YY214 pKa = 10.93 YY215 pKa = 10.53 RR216 pKa = 11.84 ALPPINPAQARR227 pKa = 11.84 QLARR231 pKa = 11.84 RR232 pKa = 11.84 LEE234 pKa = 4.22 IKK236 pKa = 9.68 TPEE239 pKa = 4.1 RR240 pKa = 11.84 LTIEE244 pKa = 4.17 SPEE247 pKa = 3.9 EE248 pKa = 3.87 SGEE251 pKa = 4.08 VVQRR255 pKa = 11.84 YY256 pKa = 7.11 EE257 pKa = 3.98 PPGGAFQRR265 pKa = 11.84 ATPDD269 pKa = 2.53 WMLPLILGLYY279 pKa = 10.53 GDD281 pKa = 4.65 ITPTWSSYY289 pKa = 10.81 LEE291 pKa = 4.48 HH292 pKa = 8.12 IEE294 pKa = 4.84 AEE296 pKa = 3.86 EE297 pKa = 4.62 DD298 pKa = 3.82 GPKK301 pKa = 10.08 KK302 pKa = 10.18 KK303 pKa = 9.95 RR304 pKa = 11.84 RR305 pKa = 11.84 KK306 pKa = 7.86 QQ307 pKa = 3.16
Molecular weight: 33.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.981
IPC2_protein 4.991
IPC_protein 4.902
Toseland 4.813
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.698
Rodwell 4.787
Grimsley 4.736
Solomon 4.851
Lehninger 4.813
Nozaki 4.965
DTASelect 5.067
Thurlkill 4.8
EMBOSS 4.724
Sillero 5.054
Patrickios 4.151
IPC_peptide 4.863
IPC2_peptide 5.041
IPC2.peptide.svr19 5.028
Protein with the highest isoelectric point:
>tr|A0A220IG84|A0A220IG84_9POLY Small T antigen OS=Giant panda polyomavirus OX=2016463 PE=4 SV=1
MM1 pKa = 7.97 DD2 pKa = 4.93 AVLTKK7 pKa = 10.6 AEE9 pKa = 4.3 KK10 pKa = 10.57 DD11 pKa = 3.44 EE12 pKa = 4.29 LMSLLGLAPTCYY24 pKa = 10.84 GNLPLMQQKK33 pKa = 9.71 YY34 pKa = 9.84 KK35 pKa = 10.33 KK36 pKa = 10.17 ASLKK40 pKa = 9.45 FHH42 pKa = 7.18 PDD44 pKa = 2.82 KK45 pKa = 11.61 GGDD48 pKa = 3.55 EE49 pKa = 4.35 EE50 pKa = 4.93 KK51 pKa = 10.28 MKK53 pKa = 10.72 RR54 pKa = 11.84 LNCLFGKK61 pKa = 10.22 VYY63 pKa = 11.07 NSFSDD68 pKa = 5.19 LRR70 pKa = 11.84 DD71 pKa = 3.43 QPRR74 pKa = 11.84 GSCSSQVNLWEE85 pKa = 4.19 KK86 pKa = 9.82 TVITTADD93 pKa = 3.32 FFGKK97 pKa = 10.15 KK98 pKa = 8.62 FEE100 pKa = 4.61 KK101 pKa = 10.71 NCVKK105 pKa = 10.31 HH106 pKa = 4.75 YY107 pKa = 10.16 HH108 pKa = 5.05 YY109 pKa = 10.62 CILEE113 pKa = 4.16 GLSKK117 pKa = 11.19 YY118 pKa = 7.4 CTCICCLLDD127 pKa = 3.74 QQHH130 pKa = 6.24 HH131 pKa = 4.27 QLKK134 pKa = 9.9 RR135 pKa = 11.84 KK136 pKa = 9.28 KK137 pKa = 10.39 SKK139 pKa = 10.09 PCLVWGEE146 pKa = 4.26 CLCFNCYY153 pKa = 10.0 RR154 pKa = 11.84 SWFGLDD160 pKa = 3.93 LNTEE164 pKa = 4.21 TLHH167 pKa = 4.91 WWKK170 pKa = 10.48 FCIYY174 pKa = 9.17 RR175 pKa = 11.84 LPMEE179 pKa = 4.52 WLNILGDD186 pKa = 3.75 IKK188 pKa = 11.12 LSDD191 pKa = 3.23 WW192 pKa = 3.63
Molecular weight: 22.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.535
IPC2_protein 7.702
IPC_protein 7.497
Toseland 7.0
ProMoST 8.097
Dawson 8.214
Bjellqvist 8.697
Wikipedia 8.112
Rodwell 8.244
Grimsley 7.0
Solomon 8.273
Lehninger 8.287
Nozaki 9.019
DTASelect 8.346
Thurlkill 8.404
EMBOSS 8.434
Sillero 8.785
Patrickios 3.872
IPC_peptide 8.258
IPC2_peptide 7.761
IPC2.peptide.svr19 7.794
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1899
192
723
379.8
42.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.161 ± 1.18
2.37 ± 0.865
5.477 ± 0.774
5.951 ± 0.591
3.739 ± 0.422
6.108 ± 0.597
1.685 ± 0.273
4.739 ± 0.516
6.161 ± 1.549
9.637 ± 0.892
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.844 ± 0.274
3.739 ± 0.525
7.846 ± 1.093
4.16 ± 0.185
4.476 ± 0.839
7.794 ± 0.463
5.74 ± 0.543
6.108 ± 0.753
1.58 ± 0.448
3.686 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here