Beihai sobemo-like virus 10
Average proteome isoelectric point is 7.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KE98|A0A1L3KE98_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 10 OX=1922681 PE=4 SV=1
MM1 pKa = 7.45 ISSIAEE7 pKa = 3.71 FLGNQLLLPFDD18 pKa = 3.9 KK19 pKa = 11.17 VVGLVAEE26 pKa = 4.42 KK27 pKa = 10.93 GKK29 pKa = 10.09 LQVPTRR35 pKa = 11.84 EE36 pKa = 4.05 SFLGYY41 pKa = 10.54 VVMAALTLAIAEE53 pKa = 4.53 FFRR56 pKa = 11.84 AHH58 pKa = 6.65 GNLCGRR64 pKa = 11.84 AYY66 pKa = 10.76 GHH68 pKa = 6.67 FLGACASLWKK78 pKa = 10.49 YY79 pKa = 10.64 CVDD82 pKa = 3.34 RR83 pKa = 11.84 MQRR86 pKa = 11.84 PATGDD91 pKa = 3.34 VASQRR96 pKa = 11.84 FGYY99 pKa = 8.81 FQHH102 pKa = 7.05 GDD104 pKa = 2.91 EE105 pKa = 5.0 HH106 pKa = 6.48 YY107 pKa = 8.35 WRR109 pKa = 11.84 CRR111 pKa = 11.84 RR112 pKa = 11.84 TSKK115 pKa = 10.66 LYY117 pKa = 10.01 RR118 pKa = 11.84 VEE120 pKa = 4.05 PGRR123 pKa = 11.84 GQFYY127 pKa = 10.36 IDD129 pKa = 3.67 DD130 pKa = 4.6 AGVEE134 pKa = 4.32 QYY136 pKa = 11.41 EE137 pKa = 4.44 VMDD140 pKa = 4.4 YY141 pKa = 11.06 IHH143 pKa = 6.73 VLVQGSEE150 pKa = 4.39 LIQPEE155 pKa = 4.13 ANLLHH160 pKa = 6.85 GEE162 pKa = 4.21 VPLPFEE168 pKa = 4.65 GLHH171 pKa = 6.06 KK172 pKa = 10.7 SVVAIFQKK180 pKa = 10.43 NHH182 pKa = 6.69 DD183 pKa = 4.05 GEE185 pKa = 4.56 FAQVGHH191 pKa = 5.84 GHH193 pKa = 6.64 RR194 pKa = 11.84 CGDD197 pKa = 3.55 YY198 pKa = 10.85 LFTNAHH204 pKa = 4.93 VHH206 pKa = 6.58 DD207 pKa = 4.37 ATNGDD212 pKa = 3.98 VYY214 pKa = 10.81 FSKK217 pKa = 10.57 DD218 pKa = 3.39 LKK220 pKa = 10.95 KK221 pKa = 10.11 FWPVGEE227 pKa = 3.73 FTYY230 pKa = 10.16 FVKK233 pKa = 10.67 NYY235 pKa = 10.52 SKK237 pKa = 10.62 ATGNDD242 pKa = 2.71 LGAYY246 pKa = 8.2 FVSRR250 pKa = 11.84 GTWAASGVSTFQATNYY266 pKa = 8.23 WPQSEE271 pKa = 4.04 GRR273 pKa = 11.84 IEE275 pKa = 3.57 ITAYY279 pKa = 10.43 DD280 pKa = 4.08 DD281 pKa = 3.62 VRR283 pKa = 11.84 GKK285 pKa = 10.34 HH286 pKa = 5.91 MIAKK290 pKa = 8.69 GHH292 pKa = 6.3 VYY294 pKa = 10.51 NQTLVDD300 pKa = 4.05 AKK302 pKa = 10.82 LGMVPHH308 pKa = 6.36 SAITKK313 pKa = 8.36 VGWSGAPVFMMKK325 pKa = 10.0 GQKK328 pKa = 8.48 RR329 pKa = 11.84 TIVGIHH335 pKa = 6.66 CGGGSDD341 pKa = 4.31 GSANYY346 pKa = 10.04 ACSIYY351 pKa = 10.68 EE352 pKa = 3.7 LLEE355 pKa = 3.57 FRR357 pKa = 11.84 RR358 pKa = 11.84 RR359 pKa = 11.84 LGLEE363 pKa = 3.46 PKK365 pKa = 10.18 IPLVCEE371 pKa = 4.17 AKK373 pKa = 10.61 FISPPSKK380 pKa = 8.93 TKK382 pKa = 10.56 QFDD385 pKa = 3.7 RR386 pKa = 11.84 YY387 pKa = 10.57 DD388 pKa = 3.59 EE389 pKa = 4.39 EE390 pKa = 4.9 SPEE393 pKa = 3.81 EE394 pKa = 3.93 RR395 pKa = 11.84 EE396 pKa = 3.9 RR397 pKa = 11.84 RR398 pKa = 11.84 EE399 pKa = 3.85 DD400 pKa = 3.63 SEE402 pKa = 4.19 MLTRR406 pKa = 11.84 EE407 pKa = 4.23 AEE409 pKa = 4.12 EE410 pKa = 4.32 HH411 pKa = 4.68 VTEE414 pKa = 4.61 LLHH417 pKa = 7.81 GEE419 pKa = 4.23 ANSPPKK425 pKa = 10.22 KK426 pKa = 10.34 DD427 pKa = 3.42 ISPEE431 pKa = 3.81 AKK433 pKa = 10.4 LPGPTWYY440 pKa = 9.61 EE441 pKa = 3.8 HH442 pKa = 5.79 CHH444 pKa = 5.68 IDD446 pKa = 4.93 APHH449 pKa = 6.42 SPAGTLVLHH458 pKa = 6.95 LADD461 pKa = 5.29 DD462 pKa = 4.22 VANMADD468 pKa = 5.61 LEE470 pKa = 4.65 DD471 pKa = 5.58 LDD473 pKa = 5.45 IPDD476 pKa = 5.37 GPPSSPDD483 pKa = 3.4 PDD485 pKa = 4.39 LDD487 pKa = 4.24 GGAADD492 pKa = 3.48 VHH494 pKa = 6.58 VNAVLAEE501 pKa = 4.15 YY502 pKa = 10.26 ILRR505 pKa = 11.84 ARR507 pKa = 11.84 AINEE511 pKa = 4.18 EE512 pKa = 4.28 VGPPPGLFPSLSEE525 pKa = 4.17 AEE527 pKa = 4.22 PPEE530 pKa = 4.47 VPQEE534 pKa = 3.8 EE535 pKa = 4.9 HH536 pKa = 6.59 EE537 pKa = 4.35 PEE539 pKa = 3.99 EE540 pKa = 4.52 HH541 pKa = 6.78 GGLSEE546 pKa = 5.02 PIYY549 pKa = 10.02 MGNPQGEE556 pKa = 4.59 PVTLGPQISPEE567 pKa = 3.81 EE568 pKa = 4.11 RR569 pKa = 11.84 EE570 pKa = 4.03 RR571 pKa = 11.84 GQKK574 pKa = 8.41 TFEE577 pKa = 4.22 SRR579 pKa = 11.84 ASRR582 pKa = 11.84 PLWAKK587 pKa = 10.41 AFLGVGLLGATALSPSGRR605 pKa = 11.84 AVTDD609 pKa = 3.56 EE610 pKa = 4.25 KK611 pKa = 11.31 ARR613 pKa = 11.84 SLLRR617 pKa = 11.84 CSPQDD622 pKa = 3.32 LAEE625 pKa = 4.16 ARR627 pKa = 11.84 KK628 pKa = 8.24 MCPISLLATPAFDD641 pKa = 4.35 RR642 pKa = 11.84 YY643 pKa = 10.13 RR644 pKa = 11.84 GYY646 pKa = 10.83 LGSGDD651 pKa = 3.71 VDD653 pKa = 3.31 WVNFEE658 pKa = 3.77 SHH660 pKa = 7.04 KK661 pKa = 10.59 IVNQSNVTVAATVGTSRR678 pKa = 11.84 LYY680 pKa = 11.03 SKK682 pKa = 7.76 EE683 pKa = 4.15 TPLTKK688 pKa = 10.61 LPGRR692 pKa = 11.84 YY693 pKa = 8.53 RR694 pKa = 11.84 EE695 pKa = 4.52 VISEE699 pKa = 4.06 MGLGPEE705 pKa = 4.47 QGFEE709 pKa = 4.45 DD710 pKa = 4.64 YY711 pKa = 11.7 VMPPSGAEE719 pKa = 3.69 AVKK722 pKa = 10.57 EE723 pKa = 4.11 SLEE726 pKa = 4.2 SQLNATCHH734 pKa = 5.49 EE735 pKa = 4.29 PWPKK739 pKa = 10.37 EE740 pKa = 3.66 VLEE743 pKa = 4.4 RR744 pKa = 11.84 DD745 pKa = 3.12 GGNYY749 pKa = 9.92 LRR751 pKa = 11.84 DD752 pKa = 3.88 EE753 pKa = 4.41 LHH755 pKa = 6.69 SEE757 pKa = 3.8 AGKK760 pKa = 10.93 YY761 pKa = 9.38 PLAFSSGISQVHH773 pKa = 6.24 DD774 pKa = 3.55 FVVKK778 pKa = 10.31 LAKK781 pKa = 10.5 EE782 pKa = 3.85 FDD784 pKa = 3.91 GTKK787 pKa = 10.07 SSGWSAHH794 pKa = 5.17 YY795 pKa = 10.52 RR796 pKa = 11.84 PGPKK800 pKa = 9.74 SAWQCAEE807 pKa = 3.7 GLEE810 pKa = 4.38 TASYY814 pKa = 10.73 LVRR817 pKa = 11.84 CRR819 pKa = 11.84 LLLRR823 pKa = 11.84 LAWGAHH829 pKa = 5.35 EE830 pKa = 4.26 MSKK833 pKa = 7.89 MTPEE837 pKa = 3.75 QMVQNGLSDD846 pKa = 3.88 PKK848 pKa = 10.37 VASIKK853 pKa = 10.41 SEE855 pKa = 3.52 PHH857 pKa = 6.05 APRR860 pKa = 11.84 KK861 pKa = 9.25 AANKK865 pKa = 7.88 KK866 pKa = 8.24 WRR868 pKa = 11.84 VIWGASIIDD877 pKa = 4.2 LLAQGVTCRR886 pKa = 11.84 IQDD889 pKa = 3.39 KK890 pKa = 11.03 LDD892 pKa = 2.95 IHH894 pKa = 7.02 SYY896 pKa = 10.4 QDD898 pKa = 3.02 GTGQHH903 pKa = 5.57 SQAVGLGHH911 pKa = 6.65 HH912 pKa = 7.2 PEE914 pKa = 5.29 GIQMIGKK921 pKa = 9.25 HH922 pKa = 5.26 IEE924 pKa = 3.74 RR925 pKa = 11.84 LQATGHH931 pKa = 5.43 QLFDD935 pKa = 4.16 ADD937 pKa = 3.61 ASGWDD942 pKa = 3.43 MSVKK946 pKa = 9.96 RR947 pKa = 11.84 DD948 pKa = 4.03 SILMDD953 pKa = 3.29 AEE955 pKa = 3.94 RR956 pKa = 11.84 RR957 pKa = 11.84 IRR959 pKa = 11.84 CYY961 pKa = 9.96 VGPYY965 pKa = 9.97 HH966 pKa = 7.17 EE967 pKa = 5.32 IFAALQMAEE976 pKa = 3.92 AMTNSAHH983 pKa = 5.91 MLAFGPYY990 pKa = 9.9 LFSIYY995 pKa = 10.23 LAGITASGILPTTSQNSFMRR1015 pKa = 11.84 AFIARR1020 pKa = 11.84 LVGIIATLCGGDD1032 pKa = 4.03 DD1033 pKa = 3.92 LMGAGKK1039 pKa = 10.07 FDD1041 pKa = 3.77 PAYY1044 pKa = 9.59 EE1045 pKa = 4.03 RR1046 pKa = 11.84 RR1047 pKa = 11.84 FGPIEE1052 pKa = 3.85 KK1053 pKa = 10.02 KK1054 pKa = 8.34 ITYY1057 pKa = 9.39 RR1058 pKa = 11.84 EE1059 pKa = 3.7 QGEE1062 pKa = 4.39 PIEE1065 pKa = 4.19 FTSFLFRR1072 pKa = 11.84 KK1073 pKa = 9.7 EE1074 pKa = 3.8 NGVWKK1079 pKa = 10.57 ADD1081 pKa = 3.55 FVNLGKK1087 pKa = 10.28 SCAKK1091 pKa = 10.18 LAFSSKK1097 pKa = 10.22 PVTPDD1102 pKa = 2.81 QLGGVLYY1109 pKa = 10.37 HH1110 pKa = 7.05 LRR1112 pKa = 11.84 DD1113 pKa = 3.74 NEE1115 pKa = 4.59 SNASLFRR1122 pKa = 11.84 EE1123 pKa = 4.68 ICRR1126 pKa = 11.84 KK1127 pKa = 8.8 MDD1129 pKa = 3.21 WPIEE1133 pKa = 4.0 GAIAGPDD1140 pKa = 3.56 GDD1142 pKa = 3.95 QGG1144 pKa = 3.58
Molecular weight: 126.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.561
IPC2_protein 5.499
IPC_protein 5.537
Toseland 5.919
ProMoST 5.855
Dawson 5.753
Bjellqvist 5.779
Wikipedia 5.741
Rodwell 5.728
Grimsley 6.071
Solomon 5.753
Lehninger 5.741
Nozaki 5.995
DTASelect 6.186
Thurlkill 6.16
EMBOSS 6.122
Sillero 6.097
Patrickios 4.037
IPC_peptide 5.766
IPC2_peptide 6.109
IPC2.peptide.svr19 6.025
Protein with the highest isoelectric point:
>tr|A0A1L3KE98|A0A1L3KE98_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 10 OX=1922681 PE=4 SV=1
MM1 pKa = 7.4 SSKK4 pKa = 10.26 GRR6 pKa = 11.84 QARR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 NRR13 pKa = 11.84 RR14 pKa = 11.84 AAAQPRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 HH23 pKa = 5.26 LKK25 pKa = 9.98 GPGGGAMSRR34 pKa = 11.84 GSRR37 pKa = 11.84 PTNRR41 pKa = 11.84 SHH43 pKa = 6.31 ATNVLATGVAAAPKK57 pKa = 9.88 KK58 pKa = 10.9 AFGCTIGHH66 pKa = 6.46 TLHH69 pKa = 6.82 CWDD72 pKa = 4.72 AKK74 pKa = 10.61 HH75 pKa = 6.05 PHH77 pKa = 6.69 HH78 pKa = 7.02 LPLPRR83 pKa = 11.84 AVGPYY88 pKa = 8.84 TVIRR92 pKa = 11.84 ATKK95 pKa = 9.93 RR96 pKa = 11.84 ITSGRR101 pKa = 11.84 AANIIGSFQFKK112 pKa = 10.27 DD113 pKa = 3.95 GASTTPDD120 pKa = 3.07 FGNWSEE126 pKa = 4.46 TIMVSDD132 pKa = 4.53 VNPTAPINQSNNTFASSVEE151 pKa = 3.99 LGGLGAAATLVPSALSVQIMCPTALQTASGVIYY184 pKa = 10.29 AGVMNTQAALAGRR197 pKa = 11.84 TEE199 pKa = 3.95 TWFSYY204 pKa = 8.96 MEE206 pKa = 4.52 KK207 pKa = 9.94 FVQFQSPRR215 pKa = 11.84 LLAASKK221 pKa = 10.15 LALRR225 pKa = 11.84 GVQINSYY232 pKa = 8.5 PLNMSEE238 pKa = 4.37 VSKK241 pKa = 9.27 FTQLKK246 pKa = 8.17 GTGDD250 pKa = 3.59 EE251 pKa = 4.14 NFAWEE256 pKa = 4.5 HH257 pKa = 5.77 FNPAGWAPIVIYY269 pKa = 10.55 NPSGTTLEE277 pKa = 3.9 MLITVEE283 pKa = 3.67 YY284 pKa = 10.05 RR285 pKa = 11.84 VRR287 pKa = 11.84 FDD289 pKa = 4.28 LDD291 pKa = 3.4 HH292 pKa = 7.03 PASASHH298 pKa = 5.65 SHH300 pKa = 6.47 HH301 pKa = 6.59 PVASDD306 pKa = 3.64 SAWDD310 pKa = 3.61 RR311 pKa = 11.84 LVRR314 pKa = 11.84 QASSLGNGVMDD325 pKa = 4.13 IADD328 pKa = 3.63 VVANAGMSVGRR339 pKa = 11.84 AIAVANRR346 pKa = 11.84 VNNVLRR352 pKa = 11.84 PLPLAAAAAA361 pKa = 4.12
Molecular weight: 38.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.619
IPC_protein 10.409
Toseland 10.613
ProMoST 10.306
Dawson 10.716
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 10.921
Grimsley 10.774
Solomon 10.833
Lehninger 10.789
Nozaki 10.599
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.628
IPC_peptide 10.833
IPC2_peptide 9.428
IPC2.peptide.svr19 8.643
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1505
361
1144
752.5
82.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.1 ± 2.014
1.462 ± 0.315
4.784 ± 0.868
6.445 ± 1.976
3.854 ± 0.265
8.97 ± 0.468
3.522 ± 0.099
4.385 ± 0.115
4.518 ± 0.736
8.04 ± 0.557
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.392 ± 0.189
3.256 ± 1.142
6.179 ± 0.043
3.522 ± 0.099
5.847 ± 0.677
7.243 ± 0.672
4.585 ± 1.032
6.246 ± 0.478
1.595 ± 0.034
3.056 ± 0.697
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here