Bat tymo-like virus
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2RVP2|A0A1B2RVP2_9VIRU RNA-directed RNA polymerase OS=Bat tymo-like virus OX=1888311 PE=4 SV=1
MM1 pKa = 7.95 ASLLPEE7 pKa = 4.62 LALAAAPIAIDD18 pKa = 3.49 AVSSLFSSPSDD29 pKa = 3.18 SSPAHH34 pKa = 5.33 VPSRR38 pKa = 11.84 IPIPRR43 pKa = 11.84 PARR46 pKa = 11.84 SSNIVSGDD54 pKa = 3.5 SMSSRR59 pKa = 11.84 DD60 pKa = 3.59 LDD62 pKa = 3.72 VSNVLSNPALPTRR75 pKa = 11.84 PRR77 pKa = 11.84 FFDD80 pKa = 3.55 VPSEE84 pKa = 4.02 LGPSINLPFQFKK96 pKa = 9.94 LHH98 pKa = 6.74 DD99 pKa = 4.38 WIGDD103 pKa = 3.65 NKK105 pKa = 10.53 LVNLDD110 pKa = 3.6 ISASEE115 pKa = 3.91 QLIKK119 pKa = 10.91 LLAPYY124 pKa = 9.94 RR125 pKa = 11.84 YY126 pKa = 9.61 AQLLSLEE133 pKa = 4.35 VLVLPRR139 pKa = 11.84 QSSSKK144 pKa = 9.88 WSGTCEE150 pKa = 3.87 VRR152 pKa = 11.84 FMPSDD157 pKa = 3.7 ATPLALDD164 pKa = 3.67 MLEE167 pKa = 4.95 HH168 pKa = 7.01 PGAMSISVGGPLGFVSNSALSCDD191 pKa = 3.57 LSKK194 pKa = 10.88 FSPVVKK200 pKa = 10.54 SPFLPTDD207 pKa = 3.75 RR208 pKa = 11.84 VRR210 pKa = 11.84 VAVNHH215 pKa = 6.22 WLNTDD220 pKa = 2.77 ATTANTQKK228 pKa = 10.74 HH229 pKa = 5.56 VIFTTLLRR237 pKa = 11.84 GTVKK241 pKa = 10.13 VAYY244 pKa = 9.25 PSFSS248 pKa = 3.12
Molecular weight: 26.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.597
IPC2_protein 6.44
IPC_protein 6.81
Toseland 6.912
ProMoST 6.942
Dawson 7.249
Bjellqvist 6.956
Wikipedia 7.234
Rodwell 7.22
Grimsley 7.015
Solomon 7.351
Lehninger 7.366
Nozaki 7.337
DTASelect 7.512
Thurlkill 7.541
EMBOSS 7.629
Sillero 7.6
Patrickios 4.711
IPC_peptide 7.366
IPC2_peptide 7.132
IPC2.peptide.svr19 7.13
Protein with the highest isoelectric point:
>tr|A0A1B2RVP2|A0A1B2RVP2_9VIRU RNA-directed RNA polymerase OS=Bat tymo-like virus OX=1888311 PE=4 SV=1
MM1 pKa = 7.29 YY2 pKa = 10.8 SEE4 pKa = 4.6 ILKK7 pKa = 8.91 STGSLHH13 pKa = 7.49 LILISKK19 pKa = 7.53 TKK21 pKa = 9.98 PLVSQTTACFQTPPSVEE38 pKa = 4.48 LPAPDD43 pKa = 4.77 CLSTPLRR50 pKa = 11.84 DD51 pKa = 3.03 RR52 pKa = 11.84 LVPKK56 pKa = 10.26 KK57 pKa = 10.54 VYY59 pKa = 10.37 EE60 pKa = 3.94 NAFQYY65 pKa = 10.04 VRR67 pKa = 11.84 SVRR70 pKa = 11.84 TLRR73 pKa = 11.84 TTDD76 pKa = 3.15 PAGYY80 pKa = 10.44 VRR82 pKa = 11.84 TQMGKK87 pKa = 9.7 EE88 pKa = 3.95 EE89 pKa = 4.37 YY90 pKa = 10.18 SWVTASAWTNLTQFILRR107 pKa = 11.84 TSALEE112 pKa = 3.81 PNSRR116 pKa = 11.84 HH117 pKa = 5.84 SLLFRR122 pKa = 11.84 PWQMLSRR129 pKa = 11.84 FLWTHH134 pKa = 5.63 SRR136 pKa = 11.84 PIHH139 pKa = 5.95 YY140 pKa = 7.47 MASLIPICISPWLSKK155 pKa = 10.5 IAVFLSNNSFNSLLFGRR172 pKa = 11.84 STIFGRR178 pKa = 11.84 PCSFDD183 pKa = 2.83 IRR185 pKa = 11.84 LYY187 pKa = 10.25 PLVSLFRR194 pKa = 11.84 PFTFFTIYY202 pKa = 10.33 RR203 pKa = 11.84 PSFVVNNPINEE214 pKa = 4.24 SLPLFLRR221 pKa = 11.84 SRR223 pKa = 11.84 ASLNFVPLVRR233 pKa = 11.84 IAFRR237 pKa = 11.84 SSTLRR242 pKa = 11.84 FFPRR246 pKa = 11.84 PHH248 pKa = 6.9 PFAVSRR254 pKa = 11.84 GTFLLAFSISILSLLHH270 pKa = 6.14 VYY272 pKa = 10.08 FRR274 pKa = 11.84 TKK276 pKa = 10.19 FHH278 pKa = 6.45 PQAVNAMFHH287 pKa = 6.9 AYY289 pKa = 9.98 CHH291 pKa = 6.6 DD292 pKa = 3.49 RR293 pKa = 11.84 LFILKK298 pKa = 7.96 TARR301 pKa = 11.84 SAVNCFPTAQPFGVTITDD319 pKa = 3.21 RR320 pKa = 11.84 SMLPSPIRR328 pKa = 11.84 LDD330 pKa = 3.95 DD331 pKa = 3.9 KK332 pKa = 11.4 AIAPPMPNHH341 pKa = 6.7 SSDD344 pKa = 2.96 IVNPIPEE351 pKa = 4.54 LASPAIEE358 pKa = 4.62 APLEE362 pKa = 4.16 PMVKK366 pKa = 10.2 AAPALDD372 pKa = 4.39 LPCSQTLCPIPDD384 pKa = 5.35 LIDD387 pKa = 3.9 LSSDD391 pKa = 3.63 VEE393 pKa = 4.14 NDD395 pKa = 3.68 PNPLTLNCLDD405 pKa = 5.33 LDD407 pKa = 4.47 PSPSGPRR414 pKa = 11.84 IINHH418 pKa = 6.07 SGCILRR424 pKa = 11.84 SSHH427 pKa = 7.01 PIVHH431 pKa = 6.32 CVSSDD436 pKa = 3.32 LSLSAGLAAQIRR448 pKa = 11.84 PFCPPDD454 pKa = 3.7 FFKK457 pKa = 8.65 MTPRR461 pKa = 11.84 VGSAVFMPSVTFGHH475 pKa = 7.31 IIHH478 pKa = 7.59 LITKK482 pKa = 8.41 PRR484 pKa = 11.84 RR485 pKa = 11.84 FDD487 pKa = 3.41 KK488 pKa = 9.55 PTLEE492 pKa = 4.1 TLRR495 pKa = 11.84 LSLINSLPQLDD506 pKa = 3.71 SHH508 pKa = 7.04 SIRR511 pKa = 11.84 EE512 pKa = 3.83 FSIPHH517 pKa = 6.67 LGCGLDD523 pKa = 3.35 HH524 pKa = 7.21 LSWNDD529 pKa = 3.6 VLPMIISLFDD539 pKa = 3.02 STYY542 pKa = 10.1 IIHH545 pKa = 7.21 IYY547 pKa = 10.11 DD548 pKa = 3.74 PSSIEE553 pKa = 3.95 SSPEE557 pKa = 3.67 PKK559 pKa = 9.5 STDD562 pKa = 2.98 SSPEE566 pKa = 3.77 LQQPPLISDD575 pKa = 4.36 DD576 pKa = 3.98 SASGPVLPWNTLNVMPDD593 pKa = 3.37 DD594 pKa = 4.41 PLISFSSRR602 pKa = 11.84 QRR604 pKa = 11.84 SNPILPPFPKK614 pKa = 10.64 SNTCLFQSISEE625 pKa = 4.15 LTEE628 pKa = 4.01 IPQSQLWTSLSSSMPNSLLVNATTTQFGFEE658 pKa = 4.52 LFHH661 pKa = 7.27 AAALCYY667 pKa = 10.17 LLRR670 pKa = 11.84 FRR672 pKa = 11.84 LHH674 pKa = 4.76 VHH676 pKa = 5.45 STQSASFIVGPVDD689 pKa = 4.43 APSLHH694 pKa = 6.28 NLYY697 pKa = 9.79 HH698 pKa = 7.27 APGHH702 pKa = 6.06 WSSVPQLSPAASTPRR717 pKa = 11.84 TRR719 pKa = 11.84 PYY721 pKa = 11.21 DD722 pKa = 3.52 AFEE725 pKa = 3.87 QALLDD730 pKa = 4.32 FKK732 pKa = 11.08 HH733 pKa = 6.02 QEE735 pKa = 3.55 AYY737 pKa = 10.9 LPFKK741 pKa = 10.35 KK742 pKa = 10.21 FHH744 pKa = 5.74 NHH746 pKa = 6.98 PINVARR752 pKa = 11.84 AKK754 pKa = 9.77 TLSSNLKK761 pKa = 9.29 NQFDD765 pKa = 4.61 GILRR769 pKa = 11.84 QVLVTSGDD777 pKa = 3.01 QKK779 pKa = 11.13 LFHH782 pKa = 6.88 KK783 pKa = 10.2 FDD785 pKa = 4.93 SIVDD789 pKa = 4.08 FSKK792 pKa = 10.45 SRR794 pKa = 11.84 SVRR797 pKa = 11.84 VCHH800 pKa = 6.73 LSGFAGCGKK809 pKa = 9.8 SAPLAAFFKK818 pKa = 9.57 NSPYY822 pKa = 10.01 IRR824 pKa = 11.84 EE825 pKa = 4.11 LRR827 pKa = 11.84 VAVPNNDD834 pKa = 5.04 LRR836 pKa = 11.84 NHH838 pKa = 5.52 WKK840 pKa = 10.39 KK841 pKa = 10.52 LLHH844 pKa = 6.5 LPNSAAWRR852 pKa = 11.84 VGTWEE857 pKa = 3.79 TSLTRR862 pKa = 11.84 RR863 pKa = 11.84 SRR865 pKa = 11.84 ILIIDD870 pKa = 5.32 EE871 pKa = 4.32 IYY873 pKa = 11.15 KK874 pKa = 9.31 MPPGYY879 pKa = 10.57 LDD881 pKa = 5.76 LICLLDD887 pKa = 3.99 PNISHH892 pKa = 6.59 VVFLGDD898 pKa = 3.76 PLQGTYY904 pKa = 10.46 HH905 pKa = 6.36 SLSSEE910 pKa = 4.23 STLKK914 pKa = 10.02 TLTPEE919 pKa = 4.44 TIVLRR924 pKa = 11.84 PYY926 pKa = 10.83 LDD928 pKa = 4.59 YY929 pKa = 11.43 YY930 pKa = 9.88 CAWTYY935 pKa = 10.8 RR936 pKa = 11.84 SPSSIARR943 pKa = 11.84 LFDD946 pKa = 3.53 VEE948 pKa = 4.58 PLSKK952 pKa = 10.84 EE953 pKa = 3.98 PGNVFYY959 pKa = 11.07 SRR961 pKa = 11.84 SHH963 pKa = 6.2 SSRR966 pKa = 11.84 LTHH969 pKa = 6.29 LTAALNSSSTLVQSGIPSQTVASSQGLSFDD999 pKa = 3.59 HH1000 pKa = 6.82 LAIHH1004 pKa = 6.79 VDD1006 pKa = 3.69 RR1007 pKa = 11.84 SWFSLSRR1014 pKa = 11.84 NLVLVALTRR1023 pKa = 11.84 HH1024 pKa = 6.26 RR1025 pKa = 11.84 KK1026 pKa = 7.81 TITITGDD1033 pKa = 2.73 ITSMRR1038 pKa = 11.84 NKK1040 pKa = 10.17 YY1041 pKa = 10.21 AFNPVFSSILDD1052 pKa = 3.76 GKK1054 pKa = 9.7 VLPLSSWSEE1063 pKa = 3.67 LDD1065 pKa = 3.33 HH1066 pKa = 8.05 ADD1068 pKa = 3.6 VLRR1071 pKa = 11.84 APLTTRR1077 pKa = 11.84 SRR1079 pKa = 11.84 TFIGGSSSFDD1089 pKa = 3.86 SISKK1093 pKa = 10.01 RR1094 pKa = 11.84 YY1095 pKa = 10.06 NRR1097 pKa = 11.84 MTNFIGDD1104 pKa = 3.67 PAHH1107 pKa = 6.76 PKK1109 pKa = 10.01 KK1110 pKa = 10.55 IPLLTPGQPRR1120 pKa = 11.84 PYY1122 pKa = 10.75 LHH1124 pKa = 6.14 TTDD1127 pKa = 4.23 HH1128 pKa = 6.27 LTDD1131 pKa = 3.96 ASHH1134 pKa = 7.18 LVFPSPGVIPHH1145 pKa = 6.6 VDD1147 pKa = 3.27 TSHH1150 pKa = 7.36 LPEE1153 pKa = 4.04 TRR1155 pKa = 11.84 RR1156 pKa = 11.84 PLHH1159 pKa = 5.87 QDD1161 pKa = 2.52 IPSALPEE1168 pKa = 4.17 PPKK1171 pKa = 10.8 LSTSDD1176 pKa = 3.38 PSSAYY1181 pKa = 9.5 IEE1183 pKa = 4.1 PVYY1186 pKa = 10.45 PGIDD1190 pKa = 3.48 SLQLMALFQEE1200 pKa = 4.54 QPLDD1204 pKa = 3.74 RR1205 pKa = 11.84 FDD1207 pKa = 6.1 SEE1209 pKa = 4.24 ILWRR1213 pKa = 11.84 EE1214 pKa = 3.76 EE1215 pKa = 4.06 FSNQFPNLDD1224 pKa = 3.66 VFSEE1228 pKa = 4.89 GSSAPNHH1235 pKa = 6.52 IAPIHH1240 pKa = 5.74 QPSKK1244 pKa = 11.25 DD1245 pKa = 3.47 PTLLPSSIKK1254 pKa = 9.83 KK1255 pKa = 9.9 RR1256 pKa = 11.84 LRR1258 pKa = 11.84 FRR1260 pKa = 11.84 PSTSPYY1266 pKa = 10.39 QLTDD1270 pKa = 3.07 HH1271 pKa = 7.63 DD1272 pKa = 4.64 ILLGHH1277 pKa = 6.2 QLFSSWCSVYY1287 pKa = 10.86 NFSTNDD1293 pKa = 3.21 TFSFDD1298 pKa = 3.56 PLLFAEE1304 pKa = 5.35 CINDD1308 pKa = 3.67 NEE1310 pKa = 4.18 YY1311 pKa = 10.83 HH1312 pKa = 6.15 QLTSKK1317 pKa = 9.78 TKK1319 pKa = 8.37 ATIAANHH1326 pKa = 5.92 EE1327 pKa = 4.19 RR1328 pKa = 11.84 SDD1330 pKa = 3.64 PSWRR1334 pKa = 11.84 HH1335 pKa = 3.21 TVVRR1339 pKa = 11.84 IFAKK1343 pKa = 10.21 SQHH1346 pKa = 6.46 KK1347 pKa = 10.77 INDD1350 pKa = 3.47 SSLFTSWKK1358 pKa = 9.88 ACQTLALAHH1367 pKa = 6.74 DD1368 pKa = 4.32 AVILLLGPVKK1378 pKa = 10.45 KK1379 pKa = 10.28 YY1380 pKa = 10.37 QRR1382 pKa = 11.84 LVFSKK1387 pKa = 10.12 KK1388 pKa = 9.73 QPPHH1392 pKa = 6.84 LFIYY1396 pKa = 10.36 ASHH1399 pKa = 6.83 TPTDD1403 pKa = 3.53 LSDD1406 pKa = 3.04 WARR1409 pKa = 11.84 IYY1411 pKa = 10.7 FSEE1414 pKa = 4.2 STPRR1418 pKa = 11.84 VCNDD1422 pKa = 2.83 YY1423 pKa = 8.96 TAFDD1427 pKa = 3.59 QSQGGEE1433 pKa = 3.78 AVIFEE1438 pKa = 4.75 LLKK1441 pKa = 9.97 MDD1443 pKa = 5.24 LAGIPNFFRR1452 pKa = 11.84 DD1453 pKa = 3.52 LHH1455 pKa = 6.29 FSLKK1459 pKa = 9.51 TRR1461 pKa = 11.84 IEE1463 pKa = 4.33 CQFGPLTSMRR1473 pKa = 11.84 LTGEE1477 pKa = 3.98 PGTYY1481 pKa = 10.26 DD1482 pKa = 5.16 DD1483 pKa = 3.95 NTDD1486 pKa = 3.26 YY1487 pKa = 11.55 NLAVLASRR1495 pKa = 11.84 FILHH1499 pKa = 6.68 GVPLCISGDD1508 pKa = 3.63 DD1509 pKa = 3.71 SALSKK1514 pKa = 11.18 VPADD1518 pKa = 3.59 NPLWPSISSRR1528 pKa = 11.84 LLLQFKK1534 pKa = 11.04 LEE1536 pKa = 3.95 ITNYY1540 pKa = 11.15 ALFCGYY1546 pKa = 10.75 YY1547 pKa = 9.41 IGPAGAIRR1555 pKa = 11.84 SPRR1558 pKa = 11.84 ALFAKK1563 pKa = 10.26 LYY1565 pKa = 9.77 IAHH1568 pKa = 7.19 NDD1570 pKa = 3.33 LSISDD1575 pKa = 3.81 KK1576 pKa = 8.84 MASYY1580 pKa = 8.88 VTEE1583 pKa = 4.45 FSVGHH1588 pKa = 5.9 SLGDD1592 pKa = 4.04 DD1593 pKa = 3.46 LWSLLPASSCIYY1605 pKa = 9.98 QAACFDD1611 pKa = 4.09 YY1612 pKa = 10.39 FCRR1615 pKa = 11.84 FAPPHH1620 pKa = 6.42 LKK1622 pKa = 10.43 DD1623 pKa = 3.18 AFKK1626 pKa = 10.55 IGEE1629 pKa = 4.37 PPSSILALVTSSLKK1643 pKa = 10.12 YY1644 pKa = 10.41 LSRR1647 pKa = 11.84 PLYY1650 pKa = 9.74 YY1651 pKa = 10.75 LLPSFLRR1658 pKa = 11.84 LRR1660 pKa = 11.84 MRR1662 pKa = 11.84 QLNPNLLPSLSPDD1675 pKa = 3.3 STPSLSYY1682 pKa = 10.81 ILL1684 pKa = 4.61
Molecular weight: 188.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.545
IPC2_protein 7.849
IPC_protein 7.614
Toseland 7.366
ProMoST 8.244
Dawson 8.346
Bjellqvist 8.741
Wikipedia 8.287
Rodwell 8.39
Grimsley 7.468
Solomon 8.375
Lehninger 8.39
Nozaki 8.902
DTASelect 8.463
Thurlkill 8.507
EMBOSS 8.551
Sillero 8.829
Patrickios 4.279
IPC_peptide 8.375
IPC2_peptide 7.761
IPC2.peptide.svr19 7.814
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1932
248
1684
966.0
107.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.315 ± 1.059
1.605 ± 0.392
5.28 ± 0.18
2.795 ± 0.185
5.694 ± 0.619
3.313 ± 0.156
3.52 ± 0.739
5.797 ± 0.67
3.468 ± 0.119
12.371 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.346 ± 0.33
3.727 ± 0.15
9.11 ± 0.279
2.795 ± 0.383
5.487 ± 0.319
12.681 ± 1.101
5.694 ± 0.619
4.969 ± 1.522
1.294 ± 0.041
2.743 ± 0.754
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here