Amino acid dipepetide frequency for Clostridium sp. KNHs216

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.178AlaAla: 10.178 ± 0.164
1.33AlaCys: 1.33 ± 0.034
4.832AlaAsp: 4.832 ± 0.073
5.696AlaGlu: 5.696 ± 0.08
3.364AlaPhe: 3.364 ± 0.055
7.306AlaGly: 7.306 ± 0.153
1.24AlaHis: 1.24 ± 0.035
5.181AlaIle: 5.181 ± 0.084
4.938AlaLys: 4.938 ± 0.065
8.364AlaLeu: 8.364 ± 0.092
2.501AlaMet: 2.501 ± 0.046
2.847AlaAsn: 2.847 ± 0.052
2.653AlaPro: 2.653 ± 0.056
3.189AlaGln: 3.189 ± 0.063
3.575AlaArg: 3.575 ± 0.062
4.754AlaSer: 4.754 ± 0.072
3.532AlaThr: 3.532 ± 0.093
7.995AlaVal: 7.995 ± 0.088
0.601AlaTrp: 0.601 ± 0.023
2.559AlaTyr: 2.559 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.395CysAla: 1.395 ± 0.037
0.333CysCys: 0.333 ± 0.02
0.891CysAsp: 0.891 ± 0.033
0.939CysGlu: 0.939 ± 0.03
0.615CysPhe: 0.615 ± 0.024
1.721CysGly: 1.721 ± 0.044
0.28CysHis: 0.28 ± 0.017
1.007CysIle: 1.007 ± 0.03
0.733CysLys: 0.733 ± 0.027
1.199CysLeu: 1.199 ± 0.034
0.436CysMet: 0.436 ± 0.023
0.518CysAsn: 0.518 ± 0.02
0.7CysPro: 0.7 ± 0.028
0.35CysGln: 0.35 ± 0.018
0.927CysArg: 0.927 ± 0.028
1.028CysSer: 1.028 ± 0.036
0.785CysThr: 0.785 ± 0.028
1.147CysVal: 1.147 ± 0.032
0.12CysTrp: 0.12 ± 0.009
0.52CysTyr: 0.52 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
4.51AspAla: 4.51 ± 0.07
0.888AspCys: 0.888 ± 0.031
2.681AspAsp: 2.681 ± 0.062
3.82AspGlu: 3.82 ± 0.06
2.605AspPhe: 2.605 ± 0.05
4.539AspGly: 4.539 ± 0.067
0.912AspHis: 0.912 ± 0.028
4.164AspIle: 4.164 ± 0.057
2.937AspLys: 2.937 ± 0.053
4.583AspLeu: 4.583 ± 0.067
1.53AspMet: 1.53 ± 0.042
1.808AspAsn: 1.808 ± 0.044
2.088AspPro: 2.088 ± 0.045
1.352AspGln: 1.352 ± 0.032
2.829AspArg: 2.829 ± 0.045
3.26AspSer: 3.26 ± 0.053
3.135AspThr: 3.135 ± 0.118
3.61AspVal: 3.61 ± 0.064
0.549AspTrp: 0.549 ± 0.021
2.195AspTyr: 2.195 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
5.079GluAla: 5.079 ± 0.069
0.797GluCys: 0.797 ± 0.028
3.195GluAsp: 3.195 ± 0.059
5.046GluGlu: 5.046 ± 0.075
2.209GluPhe: 2.209 ± 0.05
3.71GluGly: 3.71 ± 0.068
1.15GluHis: 1.15 ± 0.035
5.163GluIle: 5.163 ± 0.076
5.567GluLys: 5.567 ± 0.075
6.127GluLeu: 6.127 ± 0.078
2.045GluMet: 2.045 ± 0.038
3.693GluAsn: 3.693 ± 0.046
2.071GluPro: 2.071 ± 0.053
2.7GluGln: 2.7 ± 0.054
3.179GluArg: 3.179 ± 0.065
3.397GluSer: 3.397 ± 0.054
3.42GluThr: 3.42 ± 0.054
3.66GluVal: 3.66 ± 0.062
0.501GluTrp: 0.501 ± 0.023
2.44GluTyr: 2.44 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
3.235PheAla: 3.235 ± 0.06
0.747PheCys: 0.747 ± 0.029
2.375PheAsp: 2.375 ± 0.046
2.324PheGlu: 2.324 ± 0.043
1.861PhePhe: 1.861 ± 0.046
3.362PheGly: 3.362 ± 0.063
0.804PheHis: 0.804 ± 0.026
2.631PheIle: 2.631 ± 0.052
1.74PheLys: 1.74 ± 0.041
3.95PheLeu: 3.95 ± 0.075
0.945PheMet: 0.945 ± 0.033
1.519PheAsn: 1.519 ± 0.038
1.512PhePro: 1.512 ± 0.043
1.45PheGln: 1.45 ± 0.04
1.798PheArg: 1.798 ± 0.042
3.297PheSer: 3.297 ± 0.056
2.48PheThr: 2.48 ± 0.052
2.767PheVal: 2.767 ± 0.057
0.358PheTrp: 0.358 ± 0.018
1.514PheTyr: 1.514 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
6.32GlyAla: 6.32 ± 0.209
1.381GlyCys: 1.381 ± 0.038
3.845GlyAsp: 3.845 ± 0.103
4.751GlyGlu: 4.751 ± 0.068
3.297GlyPhe: 3.297 ± 0.064
5.874GlyGly: 5.874 ± 0.09
1.219GlyHis: 1.219 ± 0.035
6.017GlyIle: 6.017 ± 0.086
5.292GlyLys: 5.292 ± 0.073
6.558GlyLeu: 6.558 ± 0.084
2.413GlyMet: 2.413 ± 0.048
3.032GlyAsn: 3.032 ± 0.054
1.992GlyPro: 1.992 ± 0.142
2.214GlyGln: 2.214 ± 0.049
3.538GlyArg: 3.538 ± 0.055
4.537GlySer: 4.537 ± 0.077
4.722GlyThr: 4.722 ± 0.071
5.615GlyVal: 5.615 ± 0.075
0.701GlyTrp: 0.701 ± 0.027
2.956GlyTyr: 2.956 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.185HisAla: 1.185 ± 0.029
0.352HisCys: 0.352 ± 0.019
0.843HisAsp: 0.843 ± 0.03
0.937HisGlu: 0.937 ± 0.028
0.808HisPhe: 0.808 ± 0.033
1.391HisGly: 1.391 ± 0.037
0.379HisHis: 0.379 ± 0.017
1.26HisIle: 1.26 ± 0.031
0.803HisLys: 0.803 ± 0.032
1.431HisLeu: 1.431 ± 0.037
0.419HisMet: 0.419 ± 0.021
0.647HisAsn: 0.647 ± 0.02
0.971HisPro: 0.971 ± 0.033
0.517HisGln: 0.517 ± 0.023
0.858HisArg: 0.858 ± 0.029
1.047HisSer: 1.047 ± 0.032
0.967HisThr: 0.967 ± 0.031
1.07HisVal: 1.07 ± 0.031
0.163HisTrp: 0.163 ± 0.013
0.623HisTyr: 0.623 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.339IleAla: 6.339 ± 0.086
1.204IleCys: 1.204 ± 0.031
4.149IleAsp: 4.149 ± 0.062
4.052IleGlu: 4.052 ± 0.071
2.688IlePhe: 2.688 ± 0.051
5.069IleGly: 5.069 ± 0.079
1.272IleHis: 1.272 ± 0.032
4.436IleIle: 4.436 ± 0.084
3.589IleLys: 3.589 ± 0.056
6.519IleLeu: 6.519 ± 0.093
1.675IleMet: 1.675 ± 0.039
2.761IleAsn: 2.761 ± 0.049
3.181IlePro: 3.181 ± 0.063
2.197IleGln: 2.197 ± 0.046
3.662IleArg: 3.662 ± 0.055
5.045IleSer: 5.045 ± 0.077
4.143IleThr: 4.143 ± 0.066
4.963IleVal: 4.963 ± 0.074
0.459IleTrp: 0.459 ± 0.023
2.111IleTyr: 2.111 ± 0.048
0.0IleXaa: 0.0 ± 0.0
Lys
5.119LysAla: 5.119 ± 0.071
0.662LysCys: 0.662 ± 0.027
3.145LysAsp: 3.145 ± 0.053
4.57LysGlu: 4.57 ± 0.066
1.607LysPhe: 1.607 ± 0.038
3.852LysGly: 3.852 ± 0.061
0.885LysHis: 0.885 ± 0.026
4.769LysIle: 4.769 ± 0.075
4.796LysLys: 4.796 ± 0.073
5.173LysLeu: 5.173 ± 0.073
2.037LysMet: 2.037 ± 0.044
3.209LysAsn: 3.209 ± 0.056
2.171LysPro: 2.171 ± 0.044
2.165LysGln: 2.165 ± 0.05
2.823LysArg: 2.823 ± 0.052
3.574LysSer: 3.574 ± 0.048
3.805LysThr: 3.805 ± 0.057
3.582LysVal: 3.582 ± 0.064
0.535LysTrp: 0.535 ± 0.023
2.201LysTyr: 2.201 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
7.72LeuAla: 7.72 ± 0.084
1.738LeuCys: 1.738 ± 0.039
4.915LeuAsp: 4.915 ± 0.066
5.442LeuGlu: 5.442 ± 0.082
4.059LeuPhe: 4.059 ± 0.077
6.271LeuGly: 6.271 ± 0.081
1.515LeuHis: 1.515 ± 0.04
6.152LeuIle: 6.152 ± 0.087
5.925LeuLys: 5.925 ± 0.075
9.141LeuLeu: 9.141 ± 0.106
2.551LeuMet: 2.551 ± 0.049
4.048LeuAsn: 4.048 ± 0.059
3.998LeuPro: 3.998 ± 0.061
2.886LeuGln: 2.886 ± 0.051
4.321LeuArg: 4.321 ± 0.071
7.142LeuSer: 7.142 ± 0.094
5.39LeuThr: 5.39 ± 0.069
5.667LeuVal: 5.667 ± 0.079
0.703LeuTrp: 0.703 ± 0.027
2.971LeuTyr: 2.971 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.378MetAla: 2.378 ± 0.045
0.307MetCys: 0.307 ± 0.018
1.648MetAsp: 1.648 ± 0.038
2.029MetGlu: 2.029 ± 0.046
0.92MetPhe: 0.92 ± 0.031
2.108MetGly: 2.108 ± 0.048
0.397MetHis: 0.397 ± 0.019
1.908MetIle: 1.908 ± 0.039
2.246MetLys: 2.246 ± 0.047
2.76MetLeu: 2.76 ± 0.055
0.931MetMet: 0.931 ± 0.028
1.386MetAsn: 1.386 ± 0.039
1.203MetPro: 1.203 ± 0.036
0.983MetGln: 0.983 ± 0.03
1.25MetArg: 1.25 ± 0.03
1.767MetSer: 1.767 ± 0.041
1.657MetThr: 1.657 ± 0.038
1.84MetVal: 1.84 ± 0.041
0.189MetTrp: 0.189 ± 0.014
0.659MetTyr: 0.659 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.377AsnAla: 3.377 ± 0.059
0.642AsnCys: 0.642 ± 0.025
1.968AsnAsp: 1.968 ± 0.041
2.409AsnGlu: 2.409 ± 0.045
1.528AsnPhe: 1.528 ± 0.04
3.479AsnGly: 3.479 ± 0.058
0.748AsnHis: 0.748 ± 0.029
3.178AsnIle: 3.178 ± 0.063
2.077AsnLys: 2.077 ± 0.049
3.679AsnLeu: 3.679 ± 0.064
1.175AsnMet: 1.175 ± 0.036
1.596AsnAsn: 1.596 ± 0.04
2.188AsnPro: 2.188 ± 0.048
1.466AsnGln: 1.466 ± 0.039
2.111AsnArg: 2.111 ± 0.047
2.508AsnSer: 2.508 ± 0.044
2.246AsnThr: 2.246 ± 0.048
2.798AsnVal: 2.798 ± 0.051
0.344AsnTrp: 0.344 ± 0.016
1.492AsnTyr: 1.492 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
3.444ProAla: 3.444 ± 0.057
0.559ProCys: 0.559 ± 0.022
2.605ProAsp: 2.605 ± 0.058
3.222ProGlu: 3.222 ± 0.063
1.768ProPhe: 1.768 ± 0.042
2.749ProGly: 2.749 ± 0.054
0.661ProHis: 0.661 ± 0.025
2.148ProIle: 2.148 ± 0.05
1.943ProLys: 1.943 ± 0.041
3.111ProLeu: 3.111 ± 0.062
0.979ProMet: 0.979 ± 0.029
1.399ProAsn: 1.399 ± 0.037
1.158ProPro: 1.158 ± 0.036
1.447ProGln: 1.447 ± 0.041
1.332ProArg: 1.332 ± 0.037
2.202ProSer: 2.202 ± 0.047
2.074ProThr: 2.074 ± 0.144
3.597ProVal: 3.597 ± 0.056
0.329ProTrp: 0.329 ± 0.016
1.417ProTyr: 1.417 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.904GlnAla: 2.904 ± 0.052
0.366GlnCys: 0.366 ± 0.017
1.511GlnAsp: 1.511 ± 0.038
2.166GlnGlu: 2.166 ± 0.048
1.135GlnPhe: 1.135 ± 0.033
2.055GlnGly: 2.055 ± 0.047
0.457GlnHis: 0.457 ± 0.018
2.546GlnIle: 2.546 ± 0.053
2.788GlnLys: 2.788 ± 0.046
3.015GlnLeu: 3.015 ± 0.052
1.135GlnMet: 1.135 ± 0.033
1.814GlnAsn: 1.814 ± 0.046
1.21GlnPro: 1.21 ± 0.037
1.318GlnGln: 1.318 ± 0.038
1.546GlnArg: 1.546 ± 0.036
2.072GlnSer: 2.072 ± 0.043
1.849GlnThr: 1.849 ± 0.045
2.008GlnVal: 2.008 ± 0.05
0.314GlnTrp: 0.314 ± 0.015
1.265GlnTyr: 1.265 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
3.576ArgAla: 3.576 ± 0.061
0.686ArgCys: 0.686 ± 0.027
2.47ArgAsp: 2.47 ± 0.043
3.779ArgGlu: 3.779 ± 0.066
2.067ArgPhe: 2.067 ± 0.04
2.912ArgGly: 2.912 ± 0.057
0.885ArgHis: 0.885 ± 0.031
3.414ArgIle: 3.414 ± 0.053
3.113ArgLys: 3.113 ± 0.053
4.663ArgLeu: 4.663 ± 0.074
1.538ArgMet: 1.538 ± 0.034
1.953ArgAsn: 1.953 ± 0.042
1.657ArgPro: 1.657 ± 0.04
1.741ArgGln: 1.741 ± 0.04
2.721ArgArg: 2.721 ± 0.061
2.57ArgSer: 2.57 ± 0.051
2.506ArgThr: 2.506 ± 0.046
3.077ArgVal: 3.077 ± 0.056
0.41ArgTrp: 0.41 ± 0.021
1.835ArgTyr: 1.835 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
5.67SerAla: 5.67 ± 0.082
0.897SerCys: 0.897 ± 0.028
3.348SerAsp: 3.348 ± 0.068
3.746SerGlu: 3.746 ± 0.058
2.849SerPhe: 2.849 ± 0.053
6.071SerGly: 6.071 ± 0.077
0.972SerHis: 0.972 ± 0.03
4.239SerIle: 4.239 ± 0.063
3.202SerLys: 3.202 ± 0.06
5.811SerLeu: 5.811 ± 0.074
1.863SerMet: 1.863 ± 0.042
2.199SerAsn: 2.199 ± 0.046
2.301SerPro: 2.301 ± 0.053
2.035SerGln: 2.035 ± 0.042
3.09SerArg: 3.09 ± 0.056
4.553SerSer: 4.553 ± 0.089
3.235SerThr: 3.235 ± 0.049
5.022SerVal: 5.022 ± 0.074
0.473SerTrp: 0.473 ± 0.023
2.203SerTyr: 2.203 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
5.586ThrAla: 5.586 ± 0.084
0.688ThrCys: 0.688 ± 0.025
3.155ThrAsp: 3.155 ± 0.067
3.378ThrGlu: 3.378 ± 0.061
2.103ThrPhe: 2.103 ± 0.046
5.61ThrGly: 5.61 ± 0.301
0.898ThrHis: 0.898 ± 0.03
3.577ThrIle: 3.577 ± 0.06
2.645ThrLys: 2.645 ± 0.049
5.144ThrLeu: 5.144 ± 0.072
1.379ThrMet: 1.379 ± 0.036
1.895ThrAsn: 1.895 ± 0.045
2.456ThrPro: 2.456 ± 0.047
1.722ThrGln: 1.722 ± 0.047
2.151ThrArg: 2.151 ± 0.047
3.013ThrSer: 3.013 ± 0.066
2.716ThrThr: 2.716 ± 0.052
5.187ThrVal: 5.187 ± 0.095
0.392ThrTrp: 0.392 ± 0.019
1.699ThrTyr: 1.699 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
5.338ValAla: 5.338 ± 0.081
1.336ValCys: 1.336 ± 0.036
3.784ValAsp: 3.784 ± 0.065
4.2ValGlu: 4.2 ± 0.058
3.177ValPhe: 3.177 ± 0.06
4.893ValGly: 4.893 ± 0.071
1.104ValHis: 1.104 ± 0.032
4.973ValIle: 4.973 ± 0.071
4.167ValLys: 4.167 ± 0.062
7.176ValLeu: 7.176 ± 0.089
1.965ValMet: 1.965 ± 0.044
2.832ValAsn: 2.832 ± 0.054
3.012ValPro: 3.012 ± 0.059
2.16ValGln: 2.16 ± 0.041
3.413ValArg: 3.413 ± 0.055
5.363ValSer: 5.363 ± 0.074
4.345ValThr: 4.345 ± 0.084
5.064ValVal: 5.064 ± 0.069
0.597ValTrp: 0.597 ± 0.024
2.416ValTyr: 2.416 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.588TrpAla: 0.588 ± 0.023
0.116TrpCys: 0.116 ± 0.011
0.484TrpAsp: 0.484 ± 0.022
0.527TrpGlu: 0.527 ± 0.021
0.403TrpPhe: 0.403 ± 0.02
0.627TrpGly: 0.627 ± 0.026
0.171TrpHis: 0.171 ± 0.012
0.527TrpIle: 0.527 ± 0.025
0.54TrpLys: 0.54 ± 0.023
0.817TrpLeu: 0.817 ± 0.027
0.268TrpMet: 0.268 ± 0.016
0.437TrpAsn: 0.437 ± 0.02
0.239TrpPro: 0.239 ± 0.014
0.303TrpGln: 0.303 ± 0.017
0.383TrpArg: 0.383 ± 0.021
0.4TrpSer: 0.4 ± 0.02
0.424TrpThr: 0.424 ± 0.021
0.519TrpVal: 0.519 ± 0.023
0.095TrpTrp: 0.095 ± 0.01
0.298TrpTyr: 0.298 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.697TyrAla: 2.697 ± 0.049
0.618TyrCys: 0.618 ± 0.024
2.154TyrAsp: 2.154 ± 0.045
2.116TyrGlu: 2.116 ± 0.049
1.606TyrPhe: 1.606 ± 0.032
2.674TyrGly: 2.674 ± 0.041
0.72TyrHis: 0.72 ± 0.027
2.221TyrIle: 2.221 ± 0.045
1.642TyrLys: 1.642 ± 0.039
3.335TyrLeu: 3.335 ± 0.055
0.782TyrMet: 0.782 ± 0.026
1.413TyrAsn: 1.413 ± 0.04
1.384TyrPro: 1.384 ± 0.037
1.277TyrGln: 1.277 ± 0.031
2.067TyrArg: 2.067 ± 0.046
2.251TyrSer: 2.251 ± 0.044
1.962TyrThr: 1.962 ± 0.037
2.125TyrVal: 2.125 ± 0.045
0.333TyrTrp: 0.333 ± 0.017
1.406TyrTyr: 1.406 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3742 proteins (1147581 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski