Methylophaga aminisulfidivorans MP

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Methylophaga; Methylophaga aminisulfidivorans

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3174 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5T352|F5T352_9GAMM Glycine cleavage system T protein OS=Methylophaga aminisulfidivorans MP OX=1026882 GN=MAMP_01130 PE=4 SV=1
MM1 pKa = 6.92SQLYY5 pKa = 9.52FPSLSPDD12 pKa = 3.18NQTGDD17 pKa = 3.66GPASLAEE24 pKa = 4.1LQGALCGLLCMDD36 pKa = 5.48ALADD40 pKa = 4.01RR41 pKa = 11.84KK42 pKa = 9.89NWYY45 pKa = 9.26VRR47 pKa = 11.84LFEE50 pKa = 5.01DD51 pKa = 4.78FSPAEE56 pKa = 4.12EE57 pKa = 4.05EE58 pKa = 4.43KK59 pKa = 10.64QDD61 pKa = 3.7LTHH64 pKa = 7.06LFDD67 pKa = 4.19EE68 pKa = 5.9TIQSLNSLDD77 pKa = 4.06FDD79 pKa = 4.39FQIEE83 pKa = 4.41LPDD86 pKa = 4.3DD87 pKa = 3.83NAPIVSRR94 pKa = 11.84LSAMADD100 pKa = 2.82WCQGLVYY107 pKa = 10.8GLGTSGMTNEE117 pKa = 4.84TEE119 pKa = 5.28LSDD122 pKa = 4.45DD123 pKa = 3.6CQEE126 pKa = 4.2YY127 pKa = 8.19VTDD130 pKa = 4.44VIKK133 pKa = 10.68ISQVNFEE140 pKa = 4.3NVEE143 pKa = 3.97DD144 pKa = 4.3TEE146 pKa = 4.23EE147 pKa = 3.7EE148 pKa = 3.94RR149 pKa = 11.84ANFEE153 pKa = 4.09EE154 pKa = 4.22LVEE157 pKa = 4.15YY158 pKa = 10.55IRR160 pKa = 11.84MGLFLLYY167 pKa = 11.01GEE169 pKa = 5.18LQPADD174 pKa = 3.76PTDD177 pKa = 3.48EE178 pKa = 4.36ATEE181 pKa = 4.09HH182 pKa = 6.31

Molecular weight: 20.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5SYC3|F5SYC3_9GAMM Ethanolamine ammonia-lyase heavy chain OS=Methylophaga aminisulfidivorans MP OX=1026882 GN=MAMP_00513 PE=4 SV=1
MM1 pKa = 7.42SFASAVFAAVSTAGSKK17 pKa = 9.27TINISGVGEE26 pKa = 4.19MKK28 pKa = 10.02RR29 pKa = 11.84TFQPSNLKK37 pKa = 10.2RR38 pKa = 11.84KK39 pKa = 7.57RR40 pKa = 11.84THH42 pKa = 6.05GFRR45 pKa = 11.84ARR47 pKa = 11.84MKK49 pKa = 8.28TVGGRR54 pKa = 11.84RR55 pKa = 11.84VINARR60 pKa = 11.84RR61 pKa = 11.84AKK63 pKa = 9.97GRR65 pKa = 11.84ARR67 pKa = 11.84LSVV70 pKa = 3.12

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3174

0

3174

995886

37

2505

313.8

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.563 ± 0.048

0.875 ± 0.016

5.885 ± 0.043

6.272 ± 0.063

4.002 ± 0.033

6.591 ± 0.051

2.347 ± 0.025

6.668 ± 0.037

5.126 ± 0.038

10.499 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.02

4.171 ± 0.034

3.956 ± 0.032

4.673 ± 0.037

4.766 ± 0.035

6.505 ± 0.033

5.438 ± 0.031

6.728 ± 0.036

1.298 ± 0.018

2.988 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski