Vesicular stomatitis Indiana virus (strain Glasgow) (VSIV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Vesiculovirus; Indiana vesiculovirus; Vesicular stomatitis Indiana virus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P04883|GLYCO_VSIVG Glycoprotein OS=Vesicular stomatitis Indiana virus (strain Glasgow) OX=11278 GN=G PE=1 SV=1
MM1 pKa = 8.26DD2 pKa = 5.12NLTKK6 pKa = 10.36VRR8 pKa = 11.84EE9 pKa = 4.07YY10 pKa = 10.74LKK12 pKa = 10.75SYY14 pKa = 10.66SRR16 pKa = 11.84LDD18 pKa = 3.47QAVGEE23 pKa = 4.08IDD25 pKa = 3.96EE26 pKa = 5.27IEE28 pKa = 3.97AQRR31 pKa = 11.84AEE33 pKa = 3.95KK34 pKa = 10.72SNYY37 pKa = 9.35EE38 pKa = 4.03LFQEE42 pKa = 4.94DD43 pKa = 3.69GVEE46 pKa = 3.95EE47 pKa = 4.21HH48 pKa = 6.3TRR50 pKa = 11.84PSYY53 pKa = 10.41FQAADD58 pKa = 3.66DD59 pKa = 4.51SDD61 pKa = 4.18TEE63 pKa = 4.31SEE65 pKa = 5.06PEE67 pKa = 3.81IEE69 pKa = 4.85DD70 pKa = 3.56NQGLYY75 pKa = 10.63VPDD78 pKa = 4.61PEE80 pKa = 5.77AEE82 pKa = 4.03QVEE85 pKa = 4.84GFIQGPLDD93 pKa = 4.89DD94 pKa = 4.9YY95 pKa = 11.94ADD97 pKa = 4.61DD98 pKa = 4.64DD99 pKa = 4.52VDD101 pKa = 4.98VVFTSDD107 pKa = 2.64WKK109 pKa = 10.68QPEE112 pKa = 4.5LEE114 pKa = 4.1SDD116 pKa = 3.51EE117 pKa = 4.45HH118 pKa = 6.73GKK120 pKa = 7.31TLRR123 pKa = 11.84LTLPEE128 pKa = 4.38GLSGEE133 pKa = 4.62QKK135 pKa = 10.45SQWLSTIKK143 pKa = 10.75AVVQSAKK150 pKa = 9.73HH151 pKa = 5.0WNLAEE156 pKa = 4.34CTFEE160 pKa = 4.44ASGEE164 pKa = 4.03GVIIKK169 pKa = 9.83KK170 pKa = 10.18RR171 pKa = 11.84QITPDD176 pKa = 3.37VYY178 pKa = 11.16KK179 pKa = 9.69VTPVMNTHH187 pKa = 7.32PSQSEE192 pKa = 3.94AVSDD196 pKa = 3.56VWSLSKK202 pKa = 9.99TSMTFQPKK210 pKa = 9.52KK211 pKa = 10.78ASLQPLTVSLDD222 pKa = 3.49EE223 pKa = 4.8LFSSRR228 pKa = 11.84GEE230 pKa = 4.14FISVGGNGRR239 pKa = 11.84MSHH242 pKa = 6.49KK243 pKa = 10.26EE244 pKa = 3.78AILLGLRR251 pKa = 11.84YY252 pKa = 9.68KK253 pKa = 10.6KK254 pKa = 10.43LYY256 pKa = 8.53NQARR260 pKa = 11.84VKK262 pKa = 11.01YY263 pKa = 9.84SLL265 pKa = 3.74

Molecular weight: 29.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C2X6-2|C-2_VSIVG Isoform of P0C2X6 Isoform C of Protein C' OS=Vesicular stomatitis Indiana virus (strain Glasgow) OX=11278 GN=P PE=4 SV=1
MM1 pKa = 7.27SCSKK5 pKa = 9.46RR6 pKa = 11.84TEE8 pKa = 3.88WKK10 pKa = 10.46SILGPLIFRR19 pKa = 11.84QQMILTQNLNRR30 pKa = 11.84KK31 pKa = 8.94LKK33 pKa = 9.19TIKK36 pKa = 10.43ACMYY40 pKa = 9.82QIRR43 pKa = 11.84KK44 pKa = 9.18LSKK47 pKa = 10.41LKK49 pKa = 10.65ALYY52 pKa = 10.18RR53 pKa = 11.84GLL55 pKa = 3.84

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

3

8

1949

55

511

243.6

27.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.465 ± 0.651

1.385 ± 0.38

6.157 ± 0.59

5.695 ± 0.845

4.772 ± 0.491

6.414 ± 0.45

2.771 ± 0.512

5.387 ± 0.554

6.978 ± 0.558

8.415 ± 0.552

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.335 ± 0.531

3.232 ± 0.173

5.233 ± 0.339

3.284 ± 0.593

4.72 ± 0.474

8.62 ± 0.362

5.439 ± 0.326

5.285 ± 0.47

2.052 ± 0.234

4.361 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski