Desulforudis audaxviator (strain MP104C)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Candidatus Desulforudis; Candidatus Desulforudis audaxviator

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2136 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1I3Q2|B1I3Q2_DESAP Sec-independent protein translocase protein TatA OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=tatA PE=3 SV=1
MM1 pKa = 6.87QVEE4 pKa = 4.64VDD6 pKa = 3.88PEE8 pKa = 4.24LCISCGVCIEE18 pKa = 4.14MCPEE22 pKa = 3.77VFDD25 pKa = 4.49WGHH28 pKa = 5.72EE29 pKa = 4.08DD30 pKa = 3.29KK31 pKa = 11.25AVAVKK36 pKa = 11.01DD37 pKa = 3.92EE38 pKa = 4.3VPDD41 pKa = 4.25DD42 pKa = 4.27LEE44 pKa = 4.5EE45 pKa = 4.42CAHH48 pKa = 6.51EE49 pKa = 4.49AMEE52 pKa = 4.6GCPTEE57 pKa = 5.71AILEE61 pKa = 4.38HH62 pKa = 6.59

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1I6W4|B1I6W4_DESAP Resolvase N-terminal domain OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=Daud_1710 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.38KK9 pKa = 8.89RR10 pKa = 11.84KK11 pKa = 9.35RR12 pKa = 11.84KK13 pKa = 8.69RR14 pKa = 11.84LHH16 pKa = 6.02GFLIRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84NVIRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.92VLTAA44 pKa = 4.27

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2136

0

2136

657274

37

1866

307.7

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.293 ± 0.068

1.155 ± 0.022

4.836 ± 0.039

7.079 ± 0.057

3.654 ± 0.035

8.673 ± 0.053

1.98 ± 0.021

5.005 ± 0.043

3.716 ± 0.046

10.959 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.074 ± 0.023

2.577 ± 0.028

5.077 ± 0.042

3.013 ± 0.028

8.084 ± 0.054

4.299 ± 0.032

4.909 ± 0.032

8.822 ± 0.053

1.113 ± 0.023

2.683 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski