Moelleriella libera RCEF 2490
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8458 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A162I661|A0A162I661_9HYPO Uncharacterized protein OS=Moelleriella libera RCEF 2490 OX=1081109 GN=AAL_07781 PE=4 SV=1
MM1 pKa = 7.54 SFAQTSTEE9 pKa = 3.58 YY10 pKa = 10.1 WLEE13 pKa = 4.44 DD14 pKa = 3.12 GHH16 pKa = 8.23 ILHH19 pKa = 7.54 AYY21 pKa = 7.95 CQNADD26 pKa = 3.45 GDD28 pKa = 4.77 SVQSQIDD35 pKa = 3.77 LNGFIGNSDD44 pKa = 3.31 GWFEE48 pKa = 3.94 WGGVDD53 pKa = 4.45 FTEE56 pKa = 4.3 TAEE59 pKa = 4.2 NIQLEE64 pKa = 4.64 GSRR67 pKa = 11.84 LTAEE71 pKa = 5.04 LYY73 pKa = 9.54 TVEE76 pKa = 4.18 GGTRR80 pKa = 11.84 EE81 pKa = 3.96 RR82 pKa = 11.84 QGIEE86 pKa = 3.59 LNDD89 pKa = 4.64 RR90 pKa = 11.84 IGNDD94 pKa = 3.45 NGQLSYY100 pKa = 10.92 IGGG103 pKa = 3.7
Molecular weight: 11.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.935
IPC_protein 3.846
Toseland 3.668
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.694
Grimsley 3.579
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.745
Sillero 3.973
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|A0A168F3T7|A0A168F3T7_9HYPO Uncharacterized protein OS=Moelleriella libera RCEF 2490 OX=1081109 GN=AAL_02015 PE=4 SV=1
MM1 pKa = 7.22 VNHH4 pKa = 6.78 APARR8 pKa = 11.84 RR9 pKa = 11.84 PRR11 pKa = 11.84 ISAPLGPVKK20 pKa = 10.53 SSRR23 pKa = 11.84 GPDD26 pKa = 3.19 LCRR29 pKa = 11.84 SQDD32 pKa = 3.27 LRR34 pKa = 11.84 VIRR37 pKa = 11.84 SIDD40 pKa = 3.5 DD41 pKa = 3.44 CQIGYY46 pKa = 9.53 DD47 pKa = 3.59 AAAAAAAANMDD58 pKa = 4.31 PGSGAGATLTTSATTATLSSIPIASKK84 pKa = 9.21 TRR86 pKa = 11.84 FRR88 pKa = 11.84 RR89 pKa = 11.84 LSASFLSSQPPPPSTPAVAADD110 pKa = 3.85 SFIHH114 pKa = 6.85 AGPKK118 pKa = 8.49 TCFGTMPSKK127 pKa = 9.72 TARR130 pKa = 11.84 SALTSSCSSKK140 pKa = 11.05 SFLRR144 pKa = 11.84 PAVARR149 pKa = 11.84 YY150 pKa = 9.38 AFAPASSASASASASASSSSFSAYY174 pKa = 9.67 RR175 pKa = 11.84 DD176 pKa = 3.71 SPP178 pKa = 3.73
Molecular weight: 18.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.589
IPC_protein 10.394
Toseland 10.57
ProMoST 10.277
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.847
Grimsley 10.73
Solomon 10.789
Lehninger 10.76
Nozaki 10.599
DTASelect 10.394
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.599
IPC_peptide 10.789
IPC2_peptide 9.706
IPC2.peptide.svr19 8.531
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8458
0
8458
4278114
49
8775
505.8
55.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.387 ± 0.024
1.224 ± 0.01
5.927 ± 0.019
5.91 ± 0.026
3.595 ± 0.015
7.07 ± 0.027
2.466 ± 0.013
4.481 ± 0.019
4.712 ± 0.026
8.823 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.009
3.429 ± 0.015
5.965 ± 0.027
4.172 ± 0.023
6.528 ± 0.022
8.347 ± 0.031
5.713 ± 0.021
6.202 ± 0.022
1.399 ± 0.01
2.471 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here