Marinomonas spartinae
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4312 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1A8THD5|A0A1A8THD5_9GAMM Uncharacterized protein OS=Marinomonas spartinae OX=1792290 GN=MSP8886_02328 PE=4 SV=1
MM1 pKa = 6.83 NTPSIALIYY10 pKa = 9.35 GTDD13 pKa = 3.26 TSNTEE18 pKa = 3.69 EE19 pKa = 4.01 VGIKK23 pKa = 9.79 IVQQWGEE30 pKa = 4.01 LGEE33 pKa = 4.92 DD34 pKa = 3.33 VAIYY38 pKa = 10.0 NIKK41 pKa = 10.77 DD42 pKa = 3.01 VDD44 pKa = 3.88 LSIFEE49 pKa = 5.28 DD50 pKa = 4.49 YY51 pKa = 9.89 STLILGIPTWDD62 pKa = 3.3 FGGIQSDD69 pKa = 4.04 WEE71 pKa = 4.3 VLGDD75 pKa = 3.86 TLSTLSLDD83 pKa = 4.33 DD84 pKa = 3.83 KK85 pKa = 11.2 TIALYY90 pKa = 10.99 GLGDD94 pKa = 3.48 QFGYY98 pKa = 11.34 ADD100 pKa = 4.03 YY101 pKa = 10.86 FLDD104 pKa = 3.97 AMGWLYY110 pKa = 11.16 EE111 pKa = 4.05 KK112 pKa = 10.94 LKK114 pKa = 8.62 TTGATFIGQWPTEE127 pKa = 4.07 GYY129 pKa = 10.34 EE130 pKa = 4.2 FEE132 pKa = 5.26 ASQACTEE139 pKa = 4.32 DD140 pKa = 3.1 KK141 pKa = 10.42 MHH143 pKa = 6.72 FVGLAIDD150 pKa = 4.13 EE151 pKa = 4.54 DD152 pKa = 4.12 QQFEE156 pKa = 4.42 LTDD159 pKa = 3.33 QRR161 pKa = 11.84 VEE163 pKa = 3.6 QWVIQLYY170 pKa = 10.33 AEE172 pKa = 4.09 RR173 pKa = 11.84 AVLMEE178 pKa = 4.23
Molecular weight: 20.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.821
Dawson 3.668
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.986
Thurlkill 3.516
EMBOSS 3.605
Sillero 3.795
Patrickios 0.985
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A1A8T1W4|A0A1A8T1W4_9GAMM Putative glutamine ABC transporter permease protein GlnM OS=Marinomonas spartinae OX=1792290 GN=glnM_1 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.41 GGRR28 pKa = 11.84 QVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.94 ALSAA44 pKa = 4.07
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4312
0
4312
1376740
29
5643
319.3
35.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.583 ± 0.041
1.04 ± 0.013
5.415 ± 0.034
5.846 ± 0.042
4.167 ± 0.033
6.823 ± 0.038
2.425 ± 0.021
6.441 ± 0.035
5.405 ± 0.033
10.525 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.021
4.1 ± 0.037
4.068 ± 0.03
4.439 ± 0.032
4.619 ± 0.034
6.906 ± 0.037
5.369 ± 0.051
6.884 ± 0.03
1.271 ± 0.019
3.006 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here