Artogeia rapae granulovirus
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 120 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D2J4R9|D2J4R9_9BBAC DNA ligase OS=Artogeia rapae granulovirus OX=362830 PE=4 SV=1
MM1 pKa = 7.09 GQYY4 pKa = 10.84 NPIVYY9 pKa = 10.38 SKK11 pKa = 10.8 FFTLIYY17 pKa = 8.82 NTNEE21 pKa = 3.52 NAQSFDD27 pKa = 3.21 AVKK30 pKa = 10.85 NIILNSDD37 pKa = 3.86 LLKK40 pKa = 10.91 NDD42 pKa = 3.85 TDD44 pKa = 5.07 DD45 pKa = 5.87 FMLKK49 pKa = 10.26 PPVLLTPIHH58 pKa = 5.65 EE59 pKa = 4.66 LCHH62 pKa = 6.46 EE63 pKa = 4.39 EE64 pKa = 4.89 LLMLIWNAKK73 pKa = 6.42 YY74 pKa = 10.32 ARR76 pKa = 11.84 EE77 pKa = 3.85 LFLPQRR83 pKa = 11.84 WAITYY88 pKa = 7.77 YY89 pKa = 11.12 KK90 pKa = 10.49 NLEE93 pKa = 3.94 TNEE96 pKa = 4.42 CYY98 pKa = 10.27 KK99 pKa = 10.69 CSSDD103 pKa = 3.57 AQFFCVDD110 pKa = 4.72 KK111 pKa = 11.11 IITNCVNNCVKK122 pKa = 10.46 FNSFSSEE129 pKa = 4.15 DD130 pKa = 3.65 TDD132 pKa = 4.16 DD133 pKa = 3.98 LQTIVFDD140 pKa = 3.68 TDD142 pKa = 3.62 NYY144 pKa = 11.08 CVICRR149 pKa = 11.84 RR150 pKa = 11.84 ALFNIINVEE159 pKa = 4.01 NNGMFVCLDD168 pKa = 3.69 EE169 pKa = 5.07 SCFEE173 pKa = 4.17
Molecular weight: 20.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.252
IPC2_protein 4.431
IPC_protein 4.355
Toseland 4.177
ProMoST 4.431
Dawson 4.317
Bjellqvist 4.507
Wikipedia 4.215
Rodwell 4.19
Grimsley 4.088
Solomon 4.317
Lehninger 4.266
Nozaki 4.431
DTASelect 4.622
Thurlkill 4.202
EMBOSS 4.228
Sillero 4.469
Patrickios 0.604
IPC_peptide 4.317
IPC2_peptide 4.457
IPC2.peptide.svr19 4.396
Protein with the highest isoelectric point:
>tr|D2J4N9|D2J4N9_9BBAC LEF-5 OS=Artogeia rapae granulovirus OX=362830 PE=4 SV=1
MM1 pKa = 7.41 VYY3 pKa = 10.06 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 PGRR9 pKa = 11.84 PRR11 pKa = 11.84 SRR13 pKa = 11.84 SRR15 pKa = 11.84 SRR17 pKa = 11.84 SRR19 pKa = 11.84 SRR21 pKa = 11.84 SPSYY25 pKa = 10.2 RR26 pKa = 11.84 RR27 pKa = 11.84 TRR29 pKa = 11.84 TRR31 pKa = 11.84 SRR33 pKa = 11.84 SRR35 pKa = 11.84 SRR37 pKa = 11.84 SRR39 pKa = 11.84 SRR41 pKa = 11.84 SPYY44 pKa = 8.74 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 SRR49 pKa = 11.84 IQHH52 pKa = 6.84 HH53 pKa = 6.31 INQYY57 pKa = 8.01 VV58 pKa = 2.75
Molecular weight: 7.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.373
IPC2_protein 11.169
IPC_protein 12.413
Toseland 12.559
ProMoST 13.056
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.062
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 11.784
IPC_peptide 13.056
IPC2_peptide 12.047
IPC2.peptide.svr19 9.117
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
120
0
120
33172
50
1133
276.4
32.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.753 ± 0.134
2.469 ± 0.14
5.719 ± 0.16
6.189 ± 0.269
4.974 ± 0.144
3.569 ± 0.181
1.932 ± 0.094
7.684 ± 0.147
8.35 ± 0.324
9.249 ± 0.193
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.382 ± 0.11
9.237 ± 0.223
3.096 ± 0.165
3.5 ± 0.137
3.726 ± 0.14
5.758 ± 0.173
5.866 ± 0.169
6.56 ± 0.18
0.913 ± 0.071
5.074 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here