Shahe narna-like virus 3
Average proteome isoelectric point is 8.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KII2|A0A1L3KII2_9VIRU RNA-dependent RNA polymerase OS=Shahe narna-like virus 3 OX=1923431 PE=4 SV=1
MM1 pKa = 7.12 FLKK4 pKa = 10.52 RR5 pKa = 11.84 SLAPVPRR12 pKa = 11.84 SLIKK16 pKa = 10.4 KK17 pKa = 9.94 SRR19 pKa = 11.84 DD20 pKa = 3.36 DD21 pKa = 3.73 HH22 pKa = 6.9 KK23 pKa = 11.22 STLTTPSEE31 pKa = 4.14 RR32 pKa = 11.84 VHH34 pKa = 7.26 DD35 pKa = 3.98 VTLKK39 pKa = 10.91 FIEE42 pKa = 4.91 DD43 pKa = 3.85 CTTVYY48 pKa = 9.84 TRR50 pKa = 11.84 SLTKK54 pKa = 10.23 CRR56 pKa = 11.84 QEE58 pKa = 4.28 RR59 pKa = 11.84 LEE61 pKa = 4.67 KK62 pKa = 10.27 PAISPINQLKK72 pKa = 9.89 RR73 pKa = 11.84 LKK75 pKa = 9.34 RR76 pKa = 11.84 TYY78 pKa = 10.96 GPIEE82 pKa = 4.06 VKK84 pKa = 10.21 EE85 pKa = 4.22 DD86 pKa = 3.64 CLISCEE92 pKa = 4.51 DD93 pKa = 3.81 EE94 pKa = 4.15 SSHH97 pKa = 6.68 RR98 pKa = 11.84 QLDD101 pKa = 3.94 DD102 pKa = 3.67 SSLSCSEE109 pKa = 4.25 TPFLNLMATKK119 pKa = 9.74 EE120 pKa = 3.96 LHH122 pKa = 4.86 NTRR125 pKa = 11.84 DD126 pKa = 3.48 RR127 pKa = 11.84 NQVHH131 pKa = 6.54 GLHH134 pKa = 6.09 SLNRR138 pKa = 11.84 SFYY141 pKa = 8.8 PLSYY145 pKa = 10.12 KK146 pKa = 10.21 IKK148 pKa = 10.65 EE149 pKa = 3.79 VDD151 pKa = 3.32 QNLNTFDD158 pKa = 4.34 NCWIDD163 pKa = 3.56 QKK165 pKa = 11.35 VKK167 pKa = 10.33 EE168 pKa = 4.09 SSYY171 pKa = 10.58 YY172 pKa = 10.88 DD173 pKa = 3.51 KK174 pKa = 11.29 LKK176 pKa = 10.99 SDD178 pKa = 3.66 TKK180 pKa = 10.14 LTEE183 pKa = 3.88 EE184 pKa = 5.15 GEE186 pKa = 4.66 FISQQDD192 pKa = 2.94 ISMSKK197 pKa = 10.72 YY198 pKa = 10.97 SLDD201 pKa = 4.6 KK202 pKa = 10.75 INKK205 pKa = 8.76 IYY207 pKa = 10.12 PLSKK211 pKa = 9.92 HH212 pKa = 5.91 SSYY215 pKa = 11.58 NNSVKK220 pKa = 10.43 IGGAQSEE227 pKa = 4.45 ISLNLQNSNLKK238 pKa = 10.46 LGSNNGGLRR247 pKa = 11.84 KK248 pKa = 9.02 FAMLNNAMKK257 pKa = 10.34 NQYY260 pKa = 9.8 EE261 pKa = 4.09 IAKK264 pKa = 9.53 AKK266 pKa = 10.09 CLNEE270 pKa = 3.95 SKK272 pKa = 10.76 DD273 pKa = 3.51 IIKK276 pKa = 10.54 EE277 pKa = 4.14 SVRR280 pKa = 11.84 VCSIVEE286 pKa = 3.84 PLKK289 pKa = 10.93 VRR291 pKa = 11.84 TVTAEE296 pKa = 3.6 EE297 pKa = 4.39 ARR299 pKa = 11.84 NQFIKK304 pKa = 10.59 PIQKK308 pKa = 10.25 LLWEE312 pKa = 5.12 DD313 pKa = 3.88 LLSHH317 pKa = 7.04 EE318 pKa = 5.72 DD319 pKa = 4.02 FFLTRR324 pKa = 11.84 NEE326 pKa = 4.26 DD327 pKa = 5.06 FEE329 pKa = 5.18 SHH331 pKa = 5.4 WNRR334 pKa = 11.84 KK335 pKa = 7.92 FKK337 pKa = 10.88 FCDD340 pKa = 3.38 LPYY343 pKa = 10.35 HH344 pKa = 7.03 ISGDD348 pKa = 3.81 YY349 pKa = 10.19 KK350 pKa = 11.14 AATDD354 pKa = 3.66 NLNRR358 pKa = 11.84 NVINVVITQLSTLLPTNLIDD378 pKa = 6.1 LFMKK382 pKa = 10.66 NAGLHH387 pKa = 5.02 YY388 pKa = 10.59 LDD390 pKa = 4.29 YY391 pKa = 11.62 NEE393 pKa = 4.43 VAFSLRR399 pKa = 11.84 EE400 pKa = 4.07 SPDD403 pKa = 3.47 KK404 pKa = 11.18 IGDD407 pKa = 3.52 VYY409 pKa = 10.88 LQKK412 pKa = 10.74 NGQLMGSLTSFPLLCFINWISYY434 pKa = 8.03 QFSKK438 pKa = 11.23 YY439 pKa = 10.27 LVNNYY444 pKa = 9.29 PDD446 pKa = 3.34 EE447 pKa = 4.59 HH448 pKa = 8.31 LSEE451 pKa = 4.48 SCAINGDD458 pKa = 3.98 DD459 pKa = 4.46 IYY461 pKa = 11.4 FKK463 pKa = 11.23 ASLKK467 pKa = 10.71 GYY469 pKa = 10.23 AKK471 pKa = 8.86 WQEE474 pKa = 4.17 VVHH477 pKa = 6.4 SFGLSPSPGKK487 pKa = 10.38 NYY489 pKa = 10.39 SSSNCFLINSRR500 pKa = 11.84 NFLVNTKK507 pKa = 10.21 SEE509 pKa = 4.18 LKK511 pKa = 10.01 EE512 pKa = 3.64 IPFINFQMLPQYY524 pKa = 11.06 SEE526 pKa = 4.15 DD527 pKa = 3.67 SNNKK531 pKa = 9.17 FEE533 pKa = 4.84 HH534 pKa = 5.93 NKK536 pKa = 10.2 KK537 pKa = 10.09 EE538 pKa = 4.81 LLNQMKK544 pKa = 10.17 GDD546 pKa = 3.79 SVYY549 pKa = 11.2 SEE551 pKa = 4.42 VNSPIGIIFRR561 pKa = 11.84 TFCKK565 pKa = 9.67 QANINLVKK573 pKa = 10.2 PSKK576 pKa = 10.62 FDD578 pKa = 3.1 IQLIYY583 pKa = 10.86 YY584 pKa = 8.43 FYY586 pKa = 11.15 SVWKK590 pKa = 10.37 KK591 pKa = 10.66 EE592 pKa = 3.98 IIKK595 pKa = 10.28 CPRR598 pKa = 11.84 EE599 pKa = 3.94 FYY601 pKa = 10.85 LPSLIGGMGALTLDD615 pKa = 3.77 EE616 pKa = 4.6 FKK618 pKa = 10.27 KK619 pKa = 9.61 TNLLTKK625 pKa = 9.25 TKK627 pKa = 10.23 KK628 pKa = 10.48 LSLLRR633 pKa = 11.84 GFKK636 pKa = 10.39 CKK638 pKa = 10.43 SCFYY642 pKa = 8.95 MQTYY646 pKa = 8.39 EE647 pKa = 4.92 QIGSDD652 pKa = 3.31 KK653 pKa = 11.25 DD654 pKa = 3.66 QIEE657 pKa = 4.34 SFAAKK662 pKa = 10.14 LEE664 pKa = 4.15 NIILDD669 pKa = 4.15 EE670 pKa = 4.75 LSGTDD675 pKa = 4.01 PEE677 pKa = 4.33 NKK679 pKa = 9.52 QLSSKK684 pKa = 10.82 SLLSPVSTFVSKK696 pKa = 11.12 GGVEE700 pKa = 4.4 KK701 pKa = 10.45 YY702 pKa = 8.96 TLQKK706 pKa = 10.53 FFKK709 pKa = 10.18 KK710 pKa = 10.16 LGKK713 pKa = 10.12 SSFRR717 pKa = 11.84 NRR719 pKa = 11.84 KK720 pKa = 8.4 FKK722 pKa = 10.34 PATVLSIEE730 pKa = 4.22 KK731 pKa = 10.6 SLL733 pKa = 3.9
Molecular weight: 84.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.131
IPC2_protein 8.185
IPC_protein 7.995
Toseland 8.375
ProMoST 8.609
Dawson 8.946
Bjellqvist 8.975
Wikipedia 9.004
Rodwell 9.107
Grimsley 8.595
Solomon 8.99
Lehninger 8.975
Nozaki 9.151
DTASelect 8.814
Thurlkill 8.931
EMBOSS 9.121
Sillero 9.151
Patrickios 4.52
IPC_peptide 8.99
IPC2_peptide 7.644
IPC2.peptide.svr19 7.708
Protein with the highest isoelectric point:
>tr|A0A1L3KII2|A0A1L3KII2_9VIRU RNA-dependent RNA polymerase OS=Shahe narna-like virus 3 OX=1923431 PE=4 SV=1
MM1 pKa = 7.12 FLKK4 pKa = 10.52 RR5 pKa = 11.84 SLAPVPRR12 pKa = 11.84 SLIKK16 pKa = 10.4 KK17 pKa = 9.94 SRR19 pKa = 11.84 DD20 pKa = 3.36 DD21 pKa = 3.73 HH22 pKa = 6.9 KK23 pKa = 11.22 STLTTPSEE31 pKa = 4.14 RR32 pKa = 11.84 VHH34 pKa = 7.26 DD35 pKa = 3.98 VTLKK39 pKa = 10.91 FIEE42 pKa = 4.91 DD43 pKa = 3.85 CTTVYY48 pKa = 9.84 TRR50 pKa = 11.84 SLTKK54 pKa = 10.23 CRR56 pKa = 11.84 QEE58 pKa = 4.28 RR59 pKa = 11.84 LEE61 pKa = 4.67 KK62 pKa = 10.27 PAISPINQLKK72 pKa = 9.89 RR73 pKa = 11.84 LKK75 pKa = 9.34 RR76 pKa = 11.84 TYY78 pKa = 10.96 GPIEE82 pKa = 4.06 VKK84 pKa = 10.21 EE85 pKa = 4.22 DD86 pKa = 3.64 CLISCEE92 pKa = 4.51 DD93 pKa = 3.81 EE94 pKa = 4.15 SSHH97 pKa = 6.68 RR98 pKa = 11.84 QLDD101 pKa = 3.94 DD102 pKa = 3.67 SSLSCSEE109 pKa = 4.25 TPFLNLMATKK119 pKa = 9.74 EE120 pKa = 3.96 LHH122 pKa = 4.86 NTRR125 pKa = 11.84 DD126 pKa = 3.48 RR127 pKa = 11.84 NQVHH131 pKa = 6.54 GLHH134 pKa = 6.09 SLNRR138 pKa = 11.84 SFYY141 pKa = 8.8 PLSYY145 pKa = 10.12 KK146 pKa = 10.21 IKK148 pKa = 10.65 EE149 pKa = 3.79 VDD151 pKa = 3.32 QNLNTFDD158 pKa = 4.34 NCWIDD163 pKa = 3.56 QKK165 pKa = 11.35 VKK167 pKa = 10.33 EE168 pKa = 4.09 SSYY171 pKa = 10.58 YY172 pKa = 10.88 DD173 pKa = 3.51 KK174 pKa = 11.29 LKK176 pKa = 10.99 SDD178 pKa = 3.66 TKK180 pKa = 10.14 LTEE183 pKa = 3.88 EE184 pKa = 5.15 GEE186 pKa = 4.66 FISQQDD192 pKa = 2.94 ISMSKK197 pKa = 10.72 YY198 pKa = 10.97 SLDD201 pKa = 4.6 KK202 pKa = 10.75 INKK205 pKa = 8.76 IYY207 pKa = 10.12 PLSKK211 pKa = 9.92 HH212 pKa = 5.91 SSYY215 pKa = 11.58 NNSVKK220 pKa = 10.43 IGGAQSEE227 pKa = 4.45 ISLNLQNSNLKK238 pKa = 10.46 LGSNNGGLRR247 pKa = 11.84 KK248 pKa = 9.02 FAMLNNAMKK257 pKa = 10.34 NQYY260 pKa = 9.8 EE261 pKa = 4.09 IAKK264 pKa = 9.53 AKK266 pKa = 10.09 CLNEE270 pKa = 3.95 SKK272 pKa = 10.76 DD273 pKa = 3.51 IIKK276 pKa = 10.54 EE277 pKa = 4.14 SVRR280 pKa = 11.84 VCSIVEE286 pKa = 3.84 PLKK289 pKa = 10.93 VRR291 pKa = 11.84 TVTAEE296 pKa = 3.6 EE297 pKa = 4.39 ARR299 pKa = 11.84 NQFIKK304 pKa = 10.59 PIQKK308 pKa = 10.25 LLWEE312 pKa = 5.12 DD313 pKa = 3.88 LLSHH317 pKa = 7.04 EE318 pKa = 5.72 DD319 pKa = 4.02 FFLTRR324 pKa = 11.84 NEE326 pKa = 4.26 DD327 pKa = 5.06 FEE329 pKa = 5.18 SHH331 pKa = 5.4 WNRR334 pKa = 11.84 KK335 pKa = 7.92 FKK337 pKa = 10.88 FCDD340 pKa = 3.38 LPYY343 pKa = 10.35 HH344 pKa = 7.03 ISGDD348 pKa = 3.81 YY349 pKa = 10.19 KK350 pKa = 11.14 AATDD354 pKa = 3.66 NLNRR358 pKa = 11.84 NVINVVITQLSTLLPTNLIDD378 pKa = 6.1 LFMKK382 pKa = 10.66 NAGLHH387 pKa = 5.02 YY388 pKa = 10.59 LDD390 pKa = 4.29 YY391 pKa = 11.62 NEE393 pKa = 4.43 VAFSLRR399 pKa = 11.84 EE400 pKa = 4.07 SPDD403 pKa = 3.47 KK404 pKa = 11.18 IGDD407 pKa = 3.52 VYY409 pKa = 10.88 LQKK412 pKa = 10.74 NGQLMGSLTSFPLLCFINWISYY434 pKa = 8.03 QFSKK438 pKa = 11.23 YY439 pKa = 10.27 LVNNYY444 pKa = 9.29 PDD446 pKa = 3.34 EE447 pKa = 4.59 HH448 pKa = 8.31 LSEE451 pKa = 4.48 SCAINGDD458 pKa = 3.98 DD459 pKa = 4.46 IYY461 pKa = 11.4 FKK463 pKa = 11.23 ASLKK467 pKa = 10.71 GYY469 pKa = 10.23 AKK471 pKa = 8.86 WQEE474 pKa = 4.17 VVHH477 pKa = 6.4 SFGLSPSPGKK487 pKa = 10.38 NYY489 pKa = 10.39 SSSNCFLINSRR500 pKa = 11.84 NFLVNTKK507 pKa = 10.21 SEE509 pKa = 4.18 LKK511 pKa = 10.01 EE512 pKa = 3.64 IPFINFQMLPQYY524 pKa = 11.06 SEE526 pKa = 4.15 DD527 pKa = 3.67 SNNKK531 pKa = 9.17 FEE533 pKa = 4.84 HH534 pKa = 5.93 NKK536 pKa = 10.2 KK537 pKa = 10.09 EE538 pKa = 4.81 LLNQMKK544 pKa = 10.17 GDD546 pKa = 3.79 SVYY549 pKa = 11.2 SEE551 pKa = 4.42 VNSPIGIIFRR561 pKa = 11.84 TFCKK565 pKa = 9.67 QANINLVKK573 pKa = 10.2 PSKK576 pKa = 10.62 FDD578 pKa = 3.1 IQLIYY583 pKa = 10.86 YY584 pKa = 8.43 FYY586 pKa = 11.15 SVWKK590 pKa = 10.37 KK591 pKa = 10.66 EE592 pKa = 3.98 IIKK595 pKa = 10.28 CPRR598 pKa = 11.84 EE599 pKa = 3.94 FYY601 pKa = 10.85 LPSLIGGMGALTLDD615 pKa = 3.77 EE616 pKa = 4.6 FKK618 pKa = 10.27 KK619 pKa = 9.61 TNLLTKK625 pKa = 9.25 TKK627 pKa = 10.23 KK628 pKa = 10.48 LSLLRR633 pKa = 11.84 GFKK636 pKa = 10.39 CKK638 pKa = 10.43 SCFYY642 pKa = 8.95 MQTYY646 pKa = 8.39 EE647 pKa = 4.92 QIGSDD652 pKa = 3.31 KK653 pKa = 11.25 DD654 pKa = 3.66 QIEE657 pKa = 4.34 SFAAKK662 pKa = 10.14 LEE664 pKa = 4.15 NIILDD669 pKa = 4.15 EE670 pKa = 4.75 LSGTDD675 pKa = 4.01 PEE677 pKa = 4.33 NKK679 pKa = 9.52 QLSSKK684 pKa = 10.82 SLLSPVSTFVSKK696 pKa = 11.12 GGVEE700 pKa = 4.4 KK701 pKa = 10.45 YY702 pKa = 8.96 TLQKK706 pKa = 10.53 FFKK709 pKa = 10.18 KK710 pKa = 10.16 LGKK713 pKa = 10.12 SSFRR717 pKa = 11.84 NRR719 pKa = 11.84 KK720 pKa = 8.4 FKK722 pKa = 10.34 PATVLSIEE730 pKa = 4.22 KK731 pKa = 10.6 SLL733 pKa = 3.9
Molecular weight: 84.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.131
IPC2_protein 8.185
IPC_protein 7.995
Toseland 8.375
ProMoST 8.609
Dawson 8.946
Bjellqvist 8.975
Wikipedia 9.004
Rodwell 9.107
Grimsley 8.595
Solomon 8.99
Lehninger 8.975
Nozaki 9.151
DTASelect 8.814
Thurlkill 8.931
EMBOSS 9.121
Sillero 9.151
Patrickios 4.52
IPC_peptide 8.99
IPC2_peptide 7.644
IPC2.peptide.svr19 7.708
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
733
733
733
733.0
84.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.001 ± 0.0
2.183 ± 0.0
5.184 ± 0.0
6.412 ± 0.0
5.321 ± 0.0
3.82 ± 0.0
1.91 ± 0.0
6.276 ± 0.0
10.368 ± 0.0
11.187 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.501 ± 0.0
7.094 ± 0.0
3.683 ± 0.0
3.82 ± 0.0
3.683 ± 0.0
10.778 ± 0.0
4.638 ± 0.0
4.229 ± 0.0
0.819 ± 0.0
4.093 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here