Desertiactinospora gelatinilytica
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7511 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W2IC39|A0A2W2IC39_9ACTN UPF0060 membrane protein C1I98_02975 OS=Desertiactinospora gelatinilytica OX=2666298 GN=C1I98_02975 PE=3 SV=1
MM1 pKa = 7.51 FIADD5 pKa = 3.46 EE6 pKa = 4.25 VARR9 pKa = 11.84 EE10 pKa = 3.9 FAEE13 pKa = 4.32 QFNGYY18 pKa = 9.21 CADD21 pKa = 4.41 EE22 pKa = 4.04 IAARR26 pKa = 11.84 LACSEE31 pKa = 3.83 VDD33 pKa = 3.75 ALAALLTALGDD44 pKa = 3.88 EE45 pKa = 4.59 EE46 pKa = 5.87 LAATWIEE53 pKa = 3.98 YY54 pKa = 9.48 HH55 pKa = 7.28 AEE57 pKa = 4.08 GDD59 pKa = 4.97 DD60 pKa = 3.77 EE61 pKa = 6.67 DD62 pKa = 4.33 EE63 pKa = 4.12 DD64 pKa = 4.28 HH65 pKa = 6.78 YY66 pKa = 11.43 RR67 pKa = 11.84 PPSS70 pKa = 3.29
Molecular weight: 7.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.528
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.554
Grimsley 3.452
Solomon 3.668
Lehninger 3.617
Nozaki 3.821
DTASelect 3.961
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.834
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A2W2HCF6|A0A2W2HCF6_9ACTN DNA-binding response regulator OS=Desertiactinospora gelatinilytica OX=2666298 GN=C1I98_17935 PE=4 SV=1
MM1 pKa = 7.63 PGRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 ARR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 HH11 pKa = 4.94 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 PAWSGTGRR22 pKa = 11.84 LAGRR26 pKa = 11.84 PARR29 pKa = 11.84 PYY31 pKa = 10.57 GRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 CPRR38 pKa = 11.84 SARR41 pKa = 11.84 AASRR45 pKa = 11.84 GRR47 pKa = 11.84 RR48 pKa = 11.84 GAARR52 pKa = 11.84 RR53 pKa = 11.84 ARR55 pKa = 11.84 SRR57 pKa = 11.84 RR58 pKa = 11.84 WWARR62 pKa = 11.84 AAPASVRR69 pKa = 11.84 PNRR72 pKa = 11.84 PNRR75 pKa = 11.84 SAGRR79 pKa = 11.84 SGRR82 pKa = 11.84 SGRR85 pKa = 11.84 SGRR88 pKa = 11.84 SGRR91 pKa = 11.84 SGRR94 pKa = 11.84 SGRR97 pKa = 3.73
Molecular weight: 10.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.359
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.544
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.252
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.249
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7511
0
7511
2234133
27
4293
297.4
31.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.684 ± 0.039
0.823 ± 0.007
5.759 ± 0.021
5.616 ± 0.025
2.758 ± 0.015
9.529 ± 0.025
2.276 ± 0.015
3.723 ± 0.019
1.935 ± 0.02
10.446 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.9 ± 0.012
1.669 ± 0.016
6.184 ± 0.025
2.528 ± 0.016
8.637 ± 0.033
4.729 ± 0.017
5.689 ± 0.023
8.501 ± 0.026
1.526 ± 0.012
2.086 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here