Peptoniphilus ivorii
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1499 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A448V1R2|A0A448V1R2_9FIRM UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase OS=Peptoniphilus ivorii OX=54006 GN=murG PE=3 SV=1
MM1 pKa = 7.22 NRR3 pKa = 11.84 QLFFDD8 pKa = 3.75 EE9 pKa = 4.43 MGNGISFIIKK19 pKa = 9.83 ARR21 pKa = 11.84 FRR23 pKa = 11.84 VDD25 pKa = 3.14 DD26 pKa = 4.0 CDD28 pKa = 4.71 YY29 pKa = 11.18 IALLDD34 pKa = 4.56 AEE36 pKa = 5.1 HH37 pKa = 7.1 IDD39 pKa = 3.59 YY40 pKa = 8.25 GTFILRR46 pKa = 11.84 EE47 pKa = 4.14 EE48 pKa = 4.36 YY49 pKa = 10.61 DD50 pKa = 3.44 EE51 pKa = 5.57 SGDD54 pKa = 3.58 VYY56 pKa = 11.27 YY57 pKa = 11.15 AGIDD61 pKa = 3.29 EE62 pKa = 4.46 EE63 pKa = 5.01 EE64 pKa = 4.46 YY65 pKa = 10.22 VRR67 pKa = 11.84 AMEE70 pKa = 4.58 IYY72 pKa = 10.16 EE73 pKa = 4.14 EE74 pKa = 4.14 LLIEE78 pKa = 4.29 QAQQ81 pKa = 3.28
Molecular weight: 9.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.935
IPC_protein 3.859
Toseland 3.681
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.694
Grimsley 3.592
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.101
Thurlkill 3.719
EMBOSS 3.745
Sillero 3.973
Patrickios 0.896
IPC_peptide 3.821
IPC2_peptide 3.961
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|A0A448V3G6|A0A448V3G6_9FIRM CRISPR-associated exonuclease Cas4 OS=Peptoniphilus ivorii OX=54006 GN=NCTC13079_01544 PE=3 SV=1
MM1 pKa = 6.37 TTRR4 pKa = 11.84 LRR6 pKa = 11.84 RR7 pKa = 11.84 GCNPNPISKK16 pKa = 9.82 IIAVILLGTTVVRR29 pKa = 11.84 SLPDD33 pKa = 3.13 VCAWGVLSVISLFLFLNGQKK53 pKa = 8.76 RR54 pKa = 11.84 TAFRR58 pKa = 11.84 QLLLYY63 pKa = 10.38 AVLFGLPGLFALYY76 pKa = 9.71 RR77 pKa = 11.84 APFVVKK83 pKa = 9.92 MLLSLLMVIRR93 pKa = 11.84 LFFLPFAAGKK103 pKa = 9.56 FLIRR107 pKa = 11.84 TSDD110 pKa = 3.17 VGSILSSMDD119 pKa = 3.28 ALRR122 pKa = 11.84 IPASVSIPIAVIFRR136 pKa = 11.84 FFPAFRR142 pKa = 11.84 EE143 pKa = 3.83 EE144 pKa = 3.84 RR145 pKa = 11.84 RR146 pKa = 11.84 NIRR149 pKa = 11.84 TAMRR153 pKa = 11.84 IRR155 pKa = 11.84 GVLQGAPWRR164 pKa = 11.84 YY165 pKa = 8.16 PEE167 pKa = 4.28 YY168 pKa = 10.71 VAVPLLIASSEE179 pKa = 4.13 IADD182 pKa = 5.05 DD183 pKa = 3.26 IAMAAEE189 pKa = 4.93 AKK191 pKa = 10.51 CIANPVAKK199 pKa = 9.35 TRR201 pKa = 11.84 YY202 pKa = 7.08 TKK204 pKa = 10.73 VAIGAADD211 pKa = 4.49 LLYY214 pKa = 10.77 LLPIVLFTVGGWLWW228 pKa = 3.27
Molecular weight: 25.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.823
IPC_protein 10.76
Toseland 10.716
ProMoST 10.511
Dawson 10.833
Bjellqvist 10.599
Wikipedia 11.082
Rodwell 10.95
Grimsley 10.891
Solomon 10.965
Lehninger 10.921
Nozaki 10.701
DTASelect 10.584
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.76
Patrickios 10.657
IPC_peptide 10.965
IPC2_peptide 9.765
IPC2.peptide.svr19 8.392
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1499
0
1499
472416
29
2966
315.2
35.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.103 ± 0.073
0.858 ± 0.021
6.202 ± 0.054
8.013 ± 0.064
4.136 ± 0.045
7.544 ± 0.052
1.829 ± 0.03
6.983 ± 0.05
6.047 ± 0.067
9.346 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.741 ± 0.032
3.422 ± 0.042
3.733 ± 0.039
2.547 ± 0.029
5.859 ± 0.069
5.267 ± 0.048
4.933 ± 0.054
7.1 ± 0.048
0.693 ± 0.02
3.645 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here