Ralstonia phage p12J

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6UAZ7|Q6UAZ7_9VIRU Putative minor coat protein OS=Ralstonia phage p12J OX=247080 PE=4 SV=1
MM1 pKa = 7.01AQCVQIVSGQFQLDD15 pKa = 3.29SAAASSCTGYY25 pKa = 11.29LLLTADD31 pKa = 4.33EE32 pKa = 4.55VTLLHH37 pKa = 6.75AVPPLSVSDD46 pKa = 3.8GALIGSGMLGLWALAFVFRR65 pKa = 11.84SAARR69 pKa = 11.84AIYY72 pKa = 9.78QRR74 pKa = 11.84EE75 pKa = 4.05EE76 pKa = 3.78EE77 pKa = 4.22

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6UAZ4|Q6UAZ4_9VIRU Putative minor coat protein OS=Ralstonia phage p12J OX=247080 PE=4 SV=1
MM1 pKa = 7.66PFAALLASAIVGFLAQACVSLVGRR25 pKa = 11.84VLVALGIGFVMFTGVDD41 pKa = 3.21AMMTGMKK48 pKa = 9.07TLFMQYY54 pKa = 10.52VNGIGSISSVSVVGILGVLKK74 pKa = 10.81VGTSMNMILTTLGIRR89 pKa = 11.84ASLSGLSGGSIRR101 pKa = 11.84KK102 pKa = 7.94MVQKK106 pKa = 10.87

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1846

67

488

205.1

22.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.913 ± 0.938

2.275 ± 0.826

5.309 ± 0.776

4.55 ± 1.37

3.413 ± 0.684

9.317 ± 1.126

2.113 ± 0.529

3.467 ± 0.433

4.767 ± 0.997

7.042 ± 0.896

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.817 ± 0.527

2.817 ± 0.833

5.959 ± 1.26

4.442 ± 0.618

6.392 ± 1.644

6.988 ± 1.286

5.634 ± 0.795

8.776 ± 0.725

1.517 ± 0.251

2.492 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski