Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1)
Average proteome isoelectric point is 7.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P09508|CAPSD_TYYVF Major capsid protein OS=Turnip yellows virus (isolate FL-1) OX=12043 GN=ORF3 PE=1 SV=1
MM1 pKa = 7.15 YY2 pKa = 10.08 SKK4 pKa = 11.39 LMFFFALCSISFLFTSEE21 pKa = 4.24 AASTMLLEE29 pKa = 4.85 SSYY32 pKa = 11.57 LPLNQSYY39 pKa = 11.08 APGFLYY45 pKa = 10.33 KK46 pKa = 9.5 RR47 pKa = 11.84 DD48 pKa = 3.92 MLPPPLQAVLTYY60 pKa = 8.46 TCPEE64 pKa = 3.96 PRR66 pKa = 11.84 PLAEE70 pKa = 3.85 EE71 pKa = 4.55 SYY73 pKa = 11.48 NDD75 pKa = 3.77 LLRR78 pKa = 11.84 AISQKK83 pKa = 10.61 SSSDD87 pKa = 3.37 FQNAYY92 pKa = 10.72 SLALSFSSDD101 pKa = 3.69 FYY103 pKa = 11.08 QHH105 pKa = 6.33 GLKK108 pKa = 8.92 TLKK111 pKa = 10.47 DD112 pKa = 3.63 VSFLAVEE119 pKa = 4.09 KK120 pKa = 10.06 FLWGLTRR127 pKa = 11.84 LWSSLILASFSALWWLVSNFTTPVFCLALLYY158 pKa = 10.07 TVTKK162 pKa = 10.67 YY163 pKa = 8.99 MVKK166 pKa = 9.22 TVSFLFGGLPIWIISIAFSLLKK188 pKa = 10.52 KK189 pKa = 10.36 SFSALRR195 pKa = 11.84 STPKK199 pKa = 10.25 CLYY202 pKa = 9.61 EE203 pKa = 4.04 KK204 pKa = 10.62 AIDD207 pKa = 3.9 GFKK210 pKa = 10.84 SFTIPQSPPKK220 pKa = 10.29 SCVIPITHH228 pKa = 7.37 ASGNHH233 pKa = 6.04 AGYY236 pKa = 10.69 ASCIKK241 pKa = 10.48 LYY243 pKa = 10.7 NGEE246 pKa = 4.21 NALMTATHH254 pKa = 6.27 VLRR257 pKa = 11.84 DD258 pKa = 3.96 CPNAVAVSAKK268 pKa = 9.91 GLKK271 pKa = 8.58 TRR273 pKa = 11.84 IPLAEE278 pKa = 4.44 FKK280 pKa = 10.39 TIAKK284 pKa = 9.54 SDD286 pKa = 3.71 KK287 pKa = 11.22 GDD289 pKa = 3.4 VTLLRR294 pKa = 11.84 GPPNWEE300 pKa = 3.66 GLLGCKK306 pKa = 9.26 AANVITAANLAKK318 pKa = 10.05 CKK320 pKa = 10.56 ASIYY324 pKa = 10.91 SFDD327 pKa = 3.78 RR328 pKa = 11.84 DD329 pKa = 3.43 GWVSSYY335 pKa = 11.8 AEE337 pKa = 3.95 IVGSEE342 pKa = 4.18 GTDD345 pKa = 3.27 VMVLSHH351 pKa = 6.44 TEE353 pKa = 4.16 GGHH356 pKa = 6.01 SGSPYY361 pKa = 10.38 FNGKK365 pKa = 7.92 TILGVHH371 pKa = 6.43 SGASATGNYY380 pKa = 10.3 NLMAPIPSLPGLTSPTYY397 pKa = 10.69 VFEE400 pKa = 4.23 TTAPQGRR407 pKa = 11.84 VFAQEE412 pKa = 4.55 DD413 pKa = 3.38 IAEE416 pKa = 4.23 IEE418 pKa = 4.07 GLYY421 pKa = 9.26 AQVMKK426 pKa = 10.57 RR427 pKa = 11.84 VQQAEE432 pKa = 4.09 DD433 pKa = 4.06 FKK435 pKa = 11.3 PKK437 pKa = 8.96 TGKK440 pKa = 10.14 YY441 pKa = 8.0 WGDD444 pKa = 3.46 MEE446 pKa = 6.29 DD447 pKa = 4.72 DD448 pKa = 3.35 EE449 pKa = 7.03 DD450 pKa = 4.68 IFFEE454 pKa = 4.58 SKK456 pKa = 10.59 EE457 pKa = 3.98 DD458 pKa = 3.68 LSGKK462 pKa = 9.49 RR463 pKa = 11.84 SARR466 pKa = 11.84 HH467 pKa = 4.96 RR468 pKa = 11.84 PRR470 pKa = 11.84 NKK472 pKa = 9.48 RR473 pKa = 11.84 RR474 pKa = 11.84 RR475 pKa = 11.84 QLHH478 pKa = 6.14 PKK480 pKa = 9.65 DD481 pKa = 3.33 KK482 pKa = 9.52 QRR484 pKa = 11.84 RR485 pKa = 11.84 WEE487 pKa = 4.08 RR488 pKa = 11.84 DD489 pKa = 3.14 DD490 pKa = 4.91 GEE492 pKa = 4.52 NNLISSGKK500 pKa = 9.96 DD501 pKa = 2.66 KK502 pKa = 11.15 SRR504 pKa = 11.84 EE505 pKa = 3.8 HH506 pKa = 7.81 RR507 pKa = 11.84 EE508 pKa = 3.53 EE509 pKa = 3.92 SDD511 pKa = 3.34 RR512 pKa = 11.84 GDD514 pKa = 3.64 LRR516 pKa = 11.84 EE517 pKa = 3.92 SDD519 pKa = 4.39 EE520 pKa = 4.21 NSEE523 pKa = 4.19 IPPQKK528 pKa = 10.31 SPKK531 pKa = 7.56 EE532 pKa = 3.83 TAGEE536 pKa = 3.94 FEE538 pKa = 5.29 RR539 pKa = 11.84 YY540 pKa = 9.03 FSSLYY545 pKa = 9.11 NWEE548 pKa = 4.45 VPTSPRR554 pKa = 11.84 EE555 pKa = 4.01 VPGFRR560 pKa = 11.84 HH561 pKa = 6.45 CGKK564 pKa = 10.14 LPQYY568 pKa = 8.04 YY569 pKa = 9.74 HH570 pKa = 7.28 PKK572 pKa = 9.78 QKK574 pKa = 10.73 EE575 pKa = 3.74 EE576 pKa = 4.2 SSWGKK581 pKa = 7.58 TLVGNHH587 pKa = 6.7 PALGEE592 pKa = 4.15 KK593 pKa = 9.76 TSGFGWPKK601 pKa = 10.31 FGPEE605 pKa = 4.18 AEE607 pKa = 4.33 LKK609 pKa = 10.49 SLRR612 pKa = 11.84 LQASRR617 pKa = 11.84 WLEE620 pKa = 3.67 RR621 pKa = 11.84 AQSAEE626 pKa = 3.9 IPSDD630 pKa = 3.55 AEE632 pKa = 3.89 RR633 pKa = 11.84 EE634 pKa = 3.98 RR635 pKa = 11.84 VIQKK639 pKa = 8.55 TADD642 pKa = 3.38 VYY644 pKa = 11.1 HH645 pKa = 7.18 PCQTNGPAATRR656 pKa = 11.84 GGTLTWNNFMIDD668 pKa = 3.4 FKK670 pKa = 11.22 QAVFSLEE677 pKa = 3.41 FDD679 pKa = 3.58 AGIEE683 pKa = 4.07 LPYY686 pKa = 9.96 IAYY689 pKa = 8.47 GKK691 pKa = 6.48 PTHH694 pKa = 6.61 RR695 pKa = 11.84 GWVEE699 pKa = 3.84 DD700 pKa = 3.73 QKK702 pKa = 11.58 LLPILAQLTFFRR714 pKa = 11.84 LQKK717 pKa = 8.96 MLEE720 pKa = 4.17 VNFEE724 pKa = 4.3 DD725 pKa = 3.8 MGPEE729 pKa = 3.65 EE730 pKa = 4.37 LVRR733 pKa = 11.84 NGLCDD738 pKa = 4.9 PIRR741 pKa = 11.84 LFVKK745 pKa = 10.72 GEE747 pKa = 3.72 PHH749 pKa = 6.07 KK750 pKa = 10.35 QAKK753 pKa = 9.74 LDD755 pKa = 3.49 EE756 pKa = 4.35 GRR758 pKa = 11.84 YY759 pKa = 8.74 RR760 pKa = 11.84 LIMSVSLVDD769 pKa = 3.45 QLVARR774 pKa = 11.84 VLFQNQNKK782 pKa = 9.66 RR783 pKa = 11.84 EE784 pKa = 3.93 IALWRR789 pKa = 11.84 AIPSKK794 pKa = 10.55 PGFGLSTDD802 pKa = 3.63 EE803 pKa = 4.0 QVLDD807 pKa = 4.39 FVEE810 pKa = 4.52 SLARR814 pKa = 11.84 QVGTTTTEE822 pKa = 4.3 VVANWKK828 pKa = 10.48 NYY830 pKa = 7.39 LTPTDD835 pKa = 3.85 CSGFDD840 pKa = 3.29 WSVADD845 pKa = 4.66 WMLHH849 pKa = 6.3 DD850 pKa = 5.93 DD851 pKa = 3.88 MIVRR855 pKa = 11.84 NRR857 pKa = 11.84 LTIDD861 pKa = 3.98 LNPATEE867 pKa = 4.17 RR868 pKa = 11.84 LRR870 pKa = 11.84 SCWLRR875 pKa = 11.84 CISNSVLCLSDD886 pKa = 3.4 GTLLAQIHH894 pKa = 6.53 PGVQKK899 pKa = 10.58 SGSYY903 pKa = 8.25 NTSSSNSRR911 pKa = 11.84 IRR913 pKa = 11.84 VMAAFHH919 pKa = 6.38 TGAIWAMAMGDD930 pKa = 4.26 DD931 pKa = 4.27 ALEE934 pKa = 4.42 SNPADD939 pKa = 3.08 LAAYY943 pKa = 8.36 KK944 pKa = 10.67 KK945 pKa = 10.67 LGFKK949 pKa = 10.75 VEE951 pKa = 4.07 VSGQLEE957 pKa = 4.4 FCSHH961 pKa = 6.9 IFRR964 pKa = 11.84 APDD967 pKa = 2.96 LALPVNEE974 pKa = 4.54 NKK976 pKa = 9.67 MIYY979 pKa = 9.98 KK980 pKa = 10.45 LIYY983 pKa = 9.77 GYY985 pKa = 11.17 NPGSGNAEE993 pKa = 3.82 VVSNYY998 pKa = 9.89 LAACFSVLNEE1008 pKa = 4.29 LRR1010 pKa = 11.84 HH1011 pKa = 6.26 DD1012 pKa = 4.09 PASVEE1017 pKa = 4.7 LLYY1020 pKa = 11.01 SWLVDD1025 pKa = 3.26 PVLPQKK1031 pKa = 10.76 IPGEE1035 pKa = 4.12
Molecular weight: 115.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.418
IPC2_protein 6.532
IPC_protein 6.561
Toseland 6.62
ProMoST 6.927
Dawson 7.015
Bjellqvist 7.059
Wikipedia 7.0
Rodwell 7.015
Grimsley 6.722
Solomon 7.029
Lehninger 7.029
Nozaki 7.351
DTASelect 7.366
Thurlkill 7.395
EMBOSS 7.395
Sillero 7.454
Patrickios 4.253
IPC_peptide 7.044
IPC2_peptide 6.971
IPC2.peptide.svr19 6.858
Protein with the highest isoelectric point:
>sp|P09511|MVP_TYYVF Movement protein OS=Turnip yellows virus (isolate FL-1) OX=12043 GN=ORF4 PE=3 SV=1
MM1 pKa = 6.49 NTVVGRR7 pKa = 11.84 RR8 pKa = 11.84 IINGRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 PRR17 pKa = 11.84 RR18 pKa = 11.84 QTRR21 pKa = 11.84 RR22 pKa = 11.84 AQRR25 pKa = 11.84 PQPVVVVQTSRR36 pKa = 11.84 ATQRR40 pKa = 11.84 RR41 pKa = 11.84 PRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 GNNRR51 pKa = 11.84 TGRR54 pKa = 11.84 TVPTRR59 pKa = 11.84 GAGSSEE65 pKa = 3.84 TFVFSKK71 pKa = 11.13 DD72 pKa = 3.3 NLAGSSSGAITFGPSLSDD90 pKa = 4.1 CPAFSNGMLKK100 pKa = 10.2 AYY102 pKa = 10.09 HH103 pKa = 6.71 EE104 pKa = 4.71 YY105 pKa = 9.92 KK106 pKa = 10.07 ISMVILEE113 pKa = 4.63 FVSEE117 pKa = 4.2 ASSQNSGSIAYY128 pKa = 9.39 EE129 pKa = 3.85 LDD131 pKa = 3.29 PHH133 pKa = 6.98 CKK135 pKa = 10.11 LNSLSSTINKK145 pKa = 9.78 FGITKK150 pKa = 8.99 PGKK153 pKa = 8.95 RR154 pKa = 11.84 AFTASYY160 pKa = 10.69 INGTEE165 pKa = 3.71 WHH167 pKa = 7.15 DD168 pKa = 3.6 VAEE171 pKa = 4.36 DD172 pKa = 3.49 QFRR175 pKa = 11.84 ILYY178 pKa = 9.14 KK179 pKa = 11.0 GNGSSSIAGSFRR191 pKa = 11.84 ITIKK195 pKa = 10.85 CQFHH199 pKa = 6.04 NPKK202 pKa = 10.51
Molecular weight: 22.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.229
IPC2_protein 10.028
IPC_protein 11.213
Toseland 11.33
ProMoST 11.696
Dawson 11.359
Bjellqvist 11.272
Wikipedia 11.754
Rodwell 11.301
Grimsley 11.403
Solomon 11.754
Lehninger 11.667
Nozaki 11.33
DTASelect 11.272
Thurlkill 11.33
EMBOSS 11.798
Sillero 11.33
Patrickios 11.023
IPC_peptide 11.754
IPC2_peptide 10.701
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2983
45
1035
426.1
47.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.006 ± 0.584
1.475 ± 0.183
4.392 ± 0.448
5.867 ± 0.323
4.693 ± 0.792
6.839 ± 0.502
1.944 ± 0.362
4.861 ± 0.497
5.9 ± 0.497
8.683 ± 1.154
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.978 ± 0.131
4.123 ± 0.361
6.068 ± 0.406
3.553 ± 0.351
6.068 ± 0.905
10.359 ± 0.654
6.068 ± 0.507
5.33 ± 0.228
1.676 ± 0.207
3.118 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here