Amino acid dipepetide frequency for Syntrophomonas zehnderi OL-4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.121AlaAla: 8.121 ± 0.138	1.061AlaCys: 1.061 ± 0.038	4.775AlaAsp: 4.775 ± 0.077	5.422AlaGlu: 5.422 ± 0.091	2.963AlaPhe: 2.963 ± 0.068	6.697AlaGly: 6.697 ± 0.127	1.337AlaHis: 1.337 ± 0.047	5.696AlaIle: 5.696 ± 0.082	3.872AlaLys: 3.872 ± 0.084	8.026AlaLeu: 8.026 ± 0.124	2.293AlaMet: 2.293 ± 0.057	2.934AlaAsn: 2.934 ± 0.065	2.391AlaPro: 2.391 ± 0.064	3.282AlaGln: 3.282 ± 0.067	4.43AlaArg: 4.43 ± 0.075	4.102AlaSer: 4.102 ± 0.081	3.553AlaThr: 3.553 ± 0.086	6.29AlaVal: 6.29 ± 0.109	0.787AlaTrp: 0.787 ± 0.03	2.644AlaTyr: 2.644 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.852CysAla: 0.852 ± 0.033	0.206CysCys: 0.206 ± 0.017	0.62CysAsp: 0.62 ± 0.033	0.661CysGlu: 0.661 ± 0.029	0.451CysPhe: 0.451 ± 0.021	1.203CysGly: 1.203 ± 0.044	0.287CysHis: 0.287 ± 0.019	0.828CysIle: 0.828 ± 0.033	0.53CysLys: 0.53 ± 0.023	1.216CysLeu: 1.216 ± 0.034	0.312CysMet: 0.312 ± 0.019	0.461CysAsn: 0.461 ± 0.022	0.696CysPro: 0.696 ± 0.031	0.525CysGln: 0.525 ± 0.029	0.713CysArg: 0.713 ± 0.031	0.75CysSer: 0.75 ± 0.033	0.534CysThr: 0.534 ± 0.029	0.646CysVal: 0.646 ± 0.035	0.121CysTrp: 0.121 ± 0.014	0.423CysTyr: 0.423 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	3.41AspAla: 3.41 ± 0.065	0.687AspCys: 0.687 ± 0.034	2.615AspAsp: 2.615 ± 0.062	3.895AspGlu: 3.895 ± 0.083	2.533AspPhe: 2.533 ± 0.067	3.559AspGly: 3.559 ± 0.078	0.869AspHis: 0.869 ± 0.033	4.982AspIle: 4.982 ± 0.089	3.37AspLys: 3.37 ± 0.069	5.846AspLeu: 5.846 ± 0.101	1.551AspMet: 1.551 ± 0.044	2.389AspAsn: 2.389 ± 0.057	2.241AspPro: 2.241 ± 0.054	1.831AspGln: 1.831 ± 0.047	2.529AspArg: 2.529 ± 0.061	2.767AspSer: 2.767 ± 0.059	2.532AspThr: 2.532 ± 0.053	3.371AspVal: 3.371 ± 0.07	0.6AspTrp: 0.6 ± 0.033	2.429AspTyr: 2.429 ± 0.05	0.001AspXaa: 0.001 ± 0.001
Glu	5.314GluAla: 5.314 ± 0.091	0.545GluCys: 0.545 ± 0.028	3.335GluAsp: 3.335 ± 0.074	5.23GluGlu: 5.23 ± 0.092	2.209GluPhe: 2.209 ± 0.054	4.048GluGly: 4.048 ± 0.078	1.144GluHis: 1.144 ± 0.039	5.987GluIle: 5.987 ± 0.099	5.286GluLys: 5.286 ± 0.083	7.087GluLeu: 7.087 ± 0.114	2.234GluMet: 2.234 ± 0.051	3.317GluAsn: 3.317 ± 0.066	2.045GluPro: 2.045 ± 0.054	2.871GluGln: 2.871 ± 0.069	3.266GluArg: 3.266 ± 0.076	2.992GluSer: 2.992 ± 0.06	3.069GluThr: 3.069 ± 0.064	4.698GluVal: 4.698 ± 0.088	0.585GluTrp: 0.585 ± 0.028	2.368GluTyr: 2.368 ± 0.052	0.001GluXaa: 0.001 ± 0.001
Phe	3.003PheAla: 3.003 ± 0.073	0.565PheCys: 0.565 ± 0.03	2.078PheAsp: 2.078 ± 0.041	2.146PheGlu: 2.146 ± 0.052	1.659PhePhe: 1.659 ± 0.058	2.621PheGly: 2.621 ± 0.061	0.646PheHis: 0.646 ± 0.026	3.024PheIle: 3.024 ± 0.072	2.249PheLys: 2.249 ± 0.053	3.639PheLeu: 3.639 ± 0.078	1.05PheMet: 1.05 ± 0.042	1.982PheAsn: 1.982 ± 0.051	1.438PhePro: 1.438 ± 0.044	1.157PheGln: 1.157 ± 0.04	1.583PheArg: 1.583 ± 0.052	2.479PheSer: 2.479 ± 0.056	2.219PheThr: 2.219 ± 0.064	2.256PheVal: 2.256 ± 0.057	0.433PheTrp: 0.433 ± 0.025	1.419PheTyr: 1.419 ± 0.045	0.001PheXaa: 0.001 ± 0.001
Gly	5.279GlyAla: 5.279 ± 0.11	0.998GlyCys: 0.998 ± 0.038	3.548GlyAsp: 3.548 ± 0.062	4.422GlyGlu: 4.422 ± 0.07	3.099GlyPhe: 3.099 ± 0.065	5.308GlyGly: 5.308 ± 0.132	1.318GlyHis: 1.318 ± 0.043	6.151GlyIle: 6.151 ± 0.099	4.695GlyLys: 4.695 ± 0.072	7.125GlyLeu: 7.125 ± 0.106	2.191GlyMet: 2.191 ± 0.057	2.951GlyAsn: 2.951 ± 0.063	1.788GlyPro: 1.788 ± 0.052	3.052GlyGln: 3.052 ± 0.057	3.634GlyArg: 3.634 ± 0.07	4.409GlySer: 4.409 ± 0.096	3.91GlyThr: 3.91 ± 0.112	4.966GlyVal: 4.966 ± 0.09	0.74GlyTrp: 0.74 ± 0.031	2.913GlyTyr: 2.913 ± 0.059	0.002GlyXaa: 0.002 ± 0.002
His	1.053HisAla: 1.053 ± 0.039	0.276HisCys: 0.276 ± 0.021	0.877HisAsp: 0.877 ± 0.035	1.064HisGlu: 1.064 ± 0.033	0.738HisPhe: 0.738 ± 0.032	1.24HisGly: 1.24 ± 0.047	0.455HisHis: 0.455 ± 0.027	1.502HisIle: 1.502 ± 0.042	0.925HisLys: 0.925 ± 0.035	1.817HisLeu: 1.817 ± 0.048	0.468HisMet: 0.468 ± 0.027	0.804HisAsn: 0.804 ± 0.034	1.097HisPro: 1.097 ± 0.04	0.728HisGln: 0.728 ± 0.031	0.9HisArg: 0.9 ± 0.039	1.031HisSer: 1.031 ± 0.037	0.878HisThr: 0.878 ± 0.032	0.87HisVal: 0.87 ± 0.031	0.227HisTrp: 0.227 ± 0.017	0.711HisTyr: 0.711 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.317IleAla: 6.317 ± 0.092	1.065IleCys: 1.065 ± 0.035	4.46IleAsp: 4.46 ± 0.079	4.869IleGlu: 4.869 ± 0.083	2.691IlePhe: 2.691 ± 0.064	5.144IleGly: 5.144 ± 0.09	1.275IleHis: 1.275 ± 0.036	6.267IleIle: 6.267 ± 0.102	5.274IleLys: 5.274 ± 0.091	7.308IleLeu: 7.308 ± 0.105	2.19IleMet: 2.19 ± 0.057	3.971IleAsn: 3.971 ± 0.08	3.615IlePro: 3.615 ± 0.067	2.355IleGln: 2.355 ± 0.049	3.428IleArg: 3.428 ± 0.055	4.95IleSer: 4.95 ± 0.084	4.183IleThr: 4.183 ± 0.084	4.764IleVal: 4.764 ± 0.097	0.688IleTrp: 0.688 ± 0.032	2.613IleTyr: 2.613 ± 0.056	0.001IleXaa: 0.001 ± 0.001
Lys	4.89LysAla: 4.89 ± 0.079	0.533LysCys: 0.533 ± 0.026	3.35LysAsp: 3.35 ± 0.063	4.607LysGlu: 4.607 ± 0.089	1.579LysPhe: 1.579 ± 0.045	3.91LysGly: 3.91 ± 0.068	0.955LysHis: 0.955 ± 0.031	4.778LysIle: 4.778 ± 0.08	4.275LysLys: 4.275 ± 0.094	5.122LysLeu: 5.122 ± 0.077	1.917LysMet: 1.917 ± 0.048	3.232LysAsn: 3.232 ± 0.074	2.431LysPro: 2.431 ± 0.056	2.306LysGln: 2.306 ± 0.056	2.858LysArg: 2.858 ± 0.063	3.153LysSer: 3.153 ± 0.062	3.438LysThr: 3.438 ± 0.067	3.935LysVal: 3.935 ± 0.084	0.597LysTrp: 0.597 ± 0.03	2.15LysTyr: 2.15 ± 0.052	0.001LysXaa: 0.001 ± 0.001
Leu	8.861LeuAla: 8.861 ± 0.117	1.06LeuCys: 1.06 ± 0.039	5.602LeuAsp: 5.602 ± 0.085	6.699LeuGlu: 6.699 ± 0.108	3.547LeuPhe: 3.547 ± 0.088	7.241LeuGly: 7.241 ± 0.113	1.642LeuHis: 1.642 ± 0.042	6.812LeuIle: 6.812 ± 0.11	6.546LeuLys: 6.546 ± 0.1	10.151LeuLeu: 10.151 ± 0.163	2.543LeuMet: 2.543 ± 0.058	4.628LeuAsn: 4.628 ± 0.087	4.293LeuPro: 4.293 ± 0.071	3.696LeuGln: 3.696 ± 0.076	4.534LeuArg: 4.534 ± 0.08	6.241LeuSer: 6.241 ± 0.092	5.067LeuThr: 5.067 ± 0.088	6.428LeuVal: 6.428 ± 0.106	0.788LeuTrp: 0.788 ± 0.034	2.957LeuTyr: 2.957 ± 0.063	0.001LeuXaa: 0.001 ± 0.001
Met	2.634MetAla: 2.634 ± 0.06	0.247MetCys: 0.247 ± 0.019	1.595MetAsp: 1.595 ± 0.045	1.983MetGlu: 1.983 ± 0.05	0.843MetPhe: 0.843 ± 0.032	2.156MetGly: 2.156 ± 0.061	0.475MetHis: 0.475 ± 0.025	1.96MetIle: 1.96 ± 0.059	1.624MetLys: 1.624 ± 0.046	2.66MetLeu: 2.66 ± 0.056	0.725MetMet: 0.725 ± 0.027	1.286MetAsn: 1.286 ± 0.039	1.179MetPro: 1.179 ± 0.04	1.299MetGln: 1.299 ± 0.043	1.278MetArg: 1.278 ± 0.043	1.546MetSer: 1.546 ± 0.046	1.4MetThr: 1.4 ± 0.048	2.17MetVal: 2.17 ± 0.053	0.188MetTrp: 0.188 ± 0.015	0.702MetTyr: 0.702 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.057AsnAla: 3.057 ± 0.058	0.653AsnCys: 0.653 ± 0.035	2.167AsnAsp: 2.167 ± 0.052	2.725AsnGlu: 2.725 ± 0.066	1.535AsnPhe: 1.535 ± 0.037	2.996AsnGly: 2.996 ± 0.083	0.857AsnHis: 0.857 ± 0.032	3.662AsnIle: 3.662 ± 0.062	2.572AsnLys: 2.572 ± 0.062	4.536AsnLeu: 4.536 ± 0.077	1.231AsnMet: 1.231 ± 0.036	2.27AsnAsn: 2.27 ± 0.065	2.552AsnPro: 2.552 ± 0.06	2.07AsnGln: 2.07 ± 0.051	2.398AsnArg: 2.398 ± 0.053	2.557AsnSer: 2.557 ± 0.058	2.36AsnThr: 2.36 ± 0.058	2.56AsnVal: 2.56 ± 0.065	0.494AsnTrp: 0.494 ± 0.028	1.752AsnTyr: 1.752 ± 0.054	0.001AsnXaa: 0.001 ± 0.001
Pro	3.527ProAla: 3.527 ± 0.07	0.43ProCys: 0.43 ± 0.026	2.696ProAsp: 2.696 ± 0.067	3.348ProGlu: 3.348 ± 0.068	1.549ProPhe: 1.549 ± 0.044	3.343ProGly: 3.343 ± 0.08	0.827ProHis: 0.827 ± 0.03	2.368ProIle: 2.368 ± 0.061	1.615ProLys: 1.615 ± 0.049	3.718ProLeu: 3.718 ± 0.074	0.897ProMet: 0.897 ± 0.035	1.448ProAsn: 1.448 ± 0.043	1.543ProPro: 1.543 ± 0.047	1.765ProGln: 1.765 ± 0.048	1.827ProArg: 1.827 ± 0.05	2.085ProSer: 2.085 ± 0.052	1.868ProThr: 1.868 ± 0.048	3.415ProVal: 3.415 ± 0.072	0.445ProTrp: 0.445 ± 0.026	1.398ProTyr: 1.398 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	3.649GlnAla: 3.649 ± 0.073	0.388GlnCys: 0.388 ± 0.02	2.07GlnAsp: 2.07 ± 0.057	3.251GlnGlu: 3.251 ± 0.09	1.166GlnPhe: 1.166 ± 0.033	2.685GlnGly: 2.685 ± 0.061	0.651GlnHis: 0.651 ± 0.029	2.903GlnIle: 2.903 ± 0.066	2.527GlnLys: 2.527 ± 0.065	3.583GlnLeu: 3.583 ± 0.084	1.21GlnMet: 1.21 ± 0.039	1.728GlnAsn: 1.728 ± 0.052	1.452GlnPro: 1.452 ± 0.038	1.508GlnGln: 1.508 ± 0.053	1.827GlnArg: 1.827 ± 0.061	1.94GlnSer: 1.94 ± 0.054	1.98GlnThr: 1.98 ± 0.049	2.953GlnVal: 2.953 ± 0.06	0.294GlnTrp: 0.294 ± 0.019	1.246GlnTyr: 1.246 ± 0.04	0.001GlnXaa: 0.001 ± 0.001
Arg	3.19ArgAla: 3.19 ± 0.07	0.562ArgCys: 0.562 ± 0.026	2.611ArgAsp: 2.611 ± 0.066	3.789ArgGlu: 3.789 ± 0.081	1.969ArgPhe: 1.969 ± 0.05	2.989ArgGly: 2.989 ± 0.067	0.902ArgHis: 0.902 ± 0.029	4.078ArgIle: 4.078 ± 0.076	2.927ArgLys: 2.927 ± 0.061	5.097ArgLeu: 5.097 ± 0.086	1.439ArgMet: 1.439 ± 0.046	2.01ArgAsn: 2.01 ± 0.048	1.822ArgPro: 1.822 ± 0.054	2.444ArgGln: 2.444 ± 0.065	2.461ArgArg: 2.461 ± 0.067	2.433ArgSer: 2.433 ± 0.055	2.135ArgThr: 2.135 ± 0.047	3.301ArgVal: 3.301 ± 0.064	0.495ArgTrp: 0.495 ± 0.029	1.863ArgTyr: 1.863 ± 0.047	0.001ArgXaa: 0.001 ± 0.001
Ser	4.27SerAla: 4.27 ± 0.091	0.716SerCys: 0.716 ± 0.032	2.689SerAsp: 2.689 ± 0.064	3.228SerGlu: 3.228 ± 0.075	2.456SerPhe: 2.456 ± 0.057	4.956SerGly: 4.956 ± 0.112	1.07SerHis: 1.07 ± 0.042	4.17SerIle: 4.17 ± 0.074	2.914SerLys: 2.914 ± 0.057	5.93SerLeu: 5.93 ± 0.085	1.391SerMet: 1.391 ± 0.046	2.224SerAsn: 2.224 ± 0.052	2.569SerPro: 2.569 ± 0.061	2.31SerGln: 2.31 ± 0.056	3.208SerArg: 3.208 ± 0.067	3.635SerSer: 3.635 ± 0.077	2.825SerThr: 2.825 ± 0.071	3.532SerVal: 3.532 ± 0.065	0.544SerTrp: 0.544 ± 0.027	2.003SerTyr: 2.003 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	4.717ThrAla: 4.717 ± 0.11	0.547ThrCys: 0.547 ± 0.025	2.67ThrAsp: 2.67 ± 0.064	2.994ThrGlu: 2.994 ± 0.055	1.841ThrPhe: 1.841 ± 0.053	4.631ThrGly: 4.631 ± 0.084	0.907ThrHis: 0.907 ± 0.04	3.822ThrIle: 3.822 ± 0.085	2.211ThrLys: 2.211 ± 0.06	4.906ThrLeu: 4.906 ± 0.092	1.27ThrMet: 1.27 ± 0.034	1.955ThrAsn: 1.955 ± 0.063	2.393ThrPro: 2.393 ± 0.062	1.573ThrGln: 1.573 ± 0.045	2.406ThrArg: 2.406 ± 0.054	2.766ThrSer: 2.766 ± 0.069	2.7ThrThr: 2.7 ± 0.07	4.094ThrVal: 4.094 ± 0.092	0.486ThrTrp: 0.486 ± 0.024	1.674ThrTyr: 1.674 ± 0.056	0.005ThrXaa: 0.005 ± 0.003
Val	5.375ValAla: 5.375 ± 0.102	0.885ValCys: 0.885 ± 0.035	3.826ValAsp: 3.826 ± 0.071	4.445ValGlu: 4.445 ± 0.081	2.923ValPhe: 2.923 ± 0.061	4.475ValGly: 4.475 ± 0.081	1.094ValHis: 1.094 ± 0.042	5.331ValIle: 5.331 ± 0.084	4.285ValLys: 4.285 ± 0.081	6.739ValLeu: 6.739 ± 0.104	1.882ValMet: 1.882 ± 0.052	3.269ValAsn: 3.269 ± 0.063	2.644ValPro: 2.644 ± 0.055	2.115ValGln: 2.115 ± 0.055	2.962ValArg: 2.962 ± 0.069	4.268ValSer: 4.268 ± 0.088	3.615ValThr: 3.615 ± 0.076	5.077ValVal: 5.077 ± 0.094	0.589ValTrp: 0.589 ± 0.03	2.277ValTyr: 2.277 ± 0.059	0.002ValXaa: 0.002 ± 0.002
Trp	0.686TrpAla: 0.686 ± 0.028	0.1TrpCys: 0.1 ± 0.011	0.554TrpAsp: 0.554 ± 0.024	0.737TrpGlu: 0.737 ± 0.031	0.403TrpPhe: 0.403 ± 0.023	0.71TrpGly: 0.71 ± 0.031	0.219TrpHis: 0.219 ± 0.017	0.587TrpIle: 0.587 ± 0.025	0.464TrpLys: 0.464 ± 0.027	1.029TrpLeu: 1.029 ± 0.041	0.288TrpMet: 0.288 ± 0.02	0.445TrpAsn: 0.445 ± 0.025	0.343TrpPro: 0.343 ± 0.022	0.587TrpGln: 0.587 ± 0.029	0.404TrpArg: 0.404 ± 0.022	0.536TrpSer: 0.536 ± 0.03	0.424TrpThr: 0.424 ± 0.029	0.663TrpVal: 0.663 ± 0.031	0.118TrpTrp: 0.118 ± 0.013	0.314TrpTyr: 0.314 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.474TyrAla: 2.474 ± 0.058	0.51TyrCys: 0.51 ± 0.025	1.871TyrAsp: 1.871 ± 0.051	2.114TyrGlu: 2.114 ± 0.055	1.493TyrPhe: 1.493 ± 0.043	2.586TyrGly: 2.586 ± 0.051	0.732TyrHis: 0.732 ± 0.031	2.515TyrIle: 2.515 ± 0.06	1.853TyrLys: 1.853 ± 0.047	3.796TyrLeu: 3.796 ± 0.076	0.868TyrMet: 0.868 ± 0.033	1.696TyrAsn: 1.696 ± 0.049	1.58TyrPro: 1.58 ± 0.046	1.539TyrGln: 1.539 ± 0.047	1.871TyrArg: 1.871 ± 0.053	1.998TyrSer: 1.998 ± 0.046	1.878TyrThr: 1.878 ± 0.061	2.089TyrVal: 2.089 ± 0.05	0.393TyrTrp: 0.393 ± 0.023	1.548TyrTyr: 1.548 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.002	0.001XaaLys: 0.001 ± 0.001	0.005XaaLeu: 0.005 ± 0.002	0.002XaaMet: 0.002 ± 0.002	0.002XaaAsn: 0.002 ± 0.002	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.142XaaXaa: 0.142 ± 0.072

Statistics based on 2631 proteins (801858 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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