Thermoflavimicrobium dichotomicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3602 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3T481|A0A1I3T481_9BACL Cupredoxin-like domain-containing protein OS=Thermoflavimicrobium dichotomicum OX=46223 GN=SAMN05421852_11517 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 6.13GDD4 pKa = 4.23DD5 pKa = 4.8EE6 pKa = 4.74EE7 pKa = 6.68DD8 pKa = 3.5SGDD11 pKa = 3.72QEE13 pKa = 4.32VSSMISLAGTMMILTGVLEE32 pKa = 4.43NPVHH36 pKa = 6.85LFFFVHH42 pKa = 7.21FYY44 pKa = 11.54GLVV47 pKa = 3.04

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3N8F7|A0A1I3N8F7_9BACL Hydroxymethylglutaryl-CoA lyase OS=Thermoflavimicrobium dichotomicum OX=46223 GN=SAMN05421852_10423 PE=3 SV=1
MM1 pKa = 7.31NRR3 pKa = 11.84RR4 pKa = 11.84ISSVILILLSVVALVFPFIKK24 pKa = 9.07TRR26 pKa = 11.84RR27 pKa = 11.84MKK29 pKa = 10.48PLFRR33 pKa = 11.84RR34 pKa = 11.84SWSWIKK40 pKa = 10.54RR41 pKa = 11.84MKK43 pKa = 10.54LDD45 pKa = 3.3IVFGRR50 pKa = 11.84ALTNLWRR57 pKa = 11.84KK58 pKa = 10.22KK59 pKa = 9.31IMRR62 pKa = 11.84RR63 pKa = 11.84LRR65 pKa = 11.84LVV67 pKa = 3.02

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3602

0

3602

1067728

24

6425

296.4

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.19 ± 0.045

0.899 ± 0.012

4.781 ± 0.045

7.387 ± 0.053

4.164 ± 0.031

6.889 ± 0.039

2.416 ± 0.019

7.329 ± 0.045

6.495 ± 0.049

10.098 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.02

3.41 ± 0.026

4.28 ± 0.028

4.521 ± 0.032

5.104 ± 0.031

5.495 ± 0.032

4.963 ± 0.026

7.161 ± 0.035

1.316 ± 0.017

3.407 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski