Wenzhou tombus-like virus 15
Average proteome isoelectric point is 7.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGT9|A0A1L3KGT9_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 15 OX=1923668 PE=4 SV=1
MM1 pKa = 7.29 GKK3 pKa = 9.34 IVAKK7 pKa = 9.97 VMRR10 pKa = 11.84 TYY12 pKa = 10.05 TYY14 pKa = 11.05 HH15 pKa = 7.74 LIKK18 pKa = 9.98 QTDD21 pKa = 3.22 QGAVTKK27 pKa = 11.01 YY28 pKa = 8.46 MDD30 pKa = 3.83 EE31 pKa = 4.36 NAPHH35 pKa = 6.72 FPFNVKK41 pKa = 9.8 KK42 pKa = 9.73 YY43 pKa = 10.55 QPILTDD49 pKa = 3.59 PEE51 pKa = 4.41 VTNVDD56 pKa = 3.61 YY57 pKa = 11.56 ADD59 pKa = 4.83 LLFLDD64 pKa = 3.63 MRR66 pKa = 11.84 EE67 pKa = 3.75 NFARR71 pKa = 11.84 EE72 pKa = 3.86 KK73 pKa = 10.56 TRR75 pKa = 11.84 VQTTKK80 pKa = 10.43 PEE82 pKa = 4.23 HH83 pKa = 5.33 KK84 pKa = 8.6 TVRR87 pKa = 11.84 SAVSNLYY94 pKa = 8.72 GTDD97 pKa = 3.5 TPNSEE102 pKa = 3.8 QHH104 pKa = 6.0 RR105 pKa = 11.84 QFKK108 pKa = 10.71 SKK110 pKa = 10.82 ARR112 pKa = 11.84 GGKK115 pKa = 9.18 SKK117 pKa = 10.42 GRR119 pKa = 11.84 SRR121 pKa = 11.84 TFCKK125 pKa = 8.31 TTQQQHH131 pKa = 5.41 NNTYY135 pKa = 10.0 NKK137 pKa = 10.01 QNNRR141 pKa = 11.84 LQNVPQTSTRR151 pKa = 11.84 GLPTINAIVNDD162 pKa = 3.93 IIDD165 pKa = 3.46 RR166 pKa = 11.84 HH167 pKa = 5.0 TRR169 pKa = 11.84 PMSRR173 pKa = 11.84 SPVSSGRR180 pKa = 11.84 TTPSSTATATSVDD193 pKa = 4.0 TPKK196 pKa = 10.02 STTSSIKK203 pKa = 9.18 STYY206 pKa = 7.39 TTGTDD211 pKa = 3.48 PVPTSNRR218 pKa = 11.84 FIYY221 pKa = 10.51 LDD223 pKa = 3.42 YY224 pKa = 11.37 DD225 pKa = 3.54 NNEE228 pKa = 4.56 NISEE232 pKa = 4.27 DD233 pKa = 3.03 EE234 pKa = 3.75 RR235 pKa = 11.84 AYY237 pKa = 11.12 YY238 pKa = 7.86 GTRR241 pKa = 11.84 AIDD244 pKa = 3.51 RR245 pKa = 11.84 CDD247 pKa = 3.37 AACGTDD253 pKa = 3.24 GLLIEE258 pKa = 5.71 AEE260 pKa = 4.2 IQTEE264 pKa = 4.05 EE265 pKa = 4.77 DD266 pKa = 3.62 DD267 pKa = 5.84 LMIAGIKK274 pKa = 10.26 AKK276 pKa = 9.99 EE277 pKa = 4.16 KK278 pKa = 8.01 PAKK281 pKa = 9.28 YY282 pKa = 9.83 QSLHH286 pKa = 6.46 GDD288 pKa = 3.06 IEE290 pKa = 4.2 IRR292 pKa = 11.84 DD293 pKa = 4.12 FKK295 pKa = 11.33 QDD297 pKa = 2.84 IPYY300 pKa = 10.5 KK301 pKa = 10.81 LNALDD306 pKa = 3.52 NHH308 pKa = 6.21 EE309 pKa = 5.16 CFSQFKK315 pKa = 9.9 NDD317 pKa = 3.44 KK318 pKa = 9.96 LGSDD322 pKa = 5.2 FIFEE326 pKa = 4.16 PLYY329 pKa = 10.25 TYY331 pKa = 10.66 LVRR334 pKa = 11.84 HH335 pKa = 6.12 SCAKK339 pKa = 10.1 YY340 pKa = 8.92 ATRR343 pKa = 11.84 DD344 pKa = 3.67 DD345 pKa = 5.58 KK346 pKa = 11.07 IAHH349 pKa = 5.0 YY350 pKa = 10.43 NKK352 pKa = 10.21 LRR354 pKa = 11.84 MRR356 pKa = 11.84 FINEE360 pKa = 3.88 HH361 pKa = 6.52 LNHH364 pKa = 7.05 KK365 pKa = 9.82 DD366 pKa = 3.43 YY367 pKa = 11.47 KK368 pKa = 10.37 FLRR371 pKa = 11.84 EE372 pKa = 3.84 PTKK375 pKa = 9.74 QQEE378 pKa = 4.35 ILLHH382 pKa = 5.68 HH383 pKa = 6.0 TVQRR387 pKa = 11.84 CADD390 pKa = 3.75 NNEE393 pKa = 4.18 LDD395 pKa = 3.91 WIYY398 pKa = 11.14 EE399 pKa = 4.16 STKK402 pKa = 10.11 PPRR405 pKa = 11.84 EE406 pKa = 3.75 GSLRR410 pKa = 11.84 SIWRR414 pKa = 11.84 SVRR417 pKa = 11.84 RR418 pKa = 11.84 IPTRR422 pKa = 11.84 ITRR425 pKa = 11.84 VFTSTIAEE433 pKa = 4.18 SGAVEE438 pKa = 4.2 NNVTSTIEE446 pKa = 4.16 NIPKK450 pKa = 8.05 YY451 pKa = 9.76 CYY453 pKa = 9.43 WSGSHH458 pKa = 6.33 PTQEE462 pKa = 5.38 DD463 pKa = 3.69 NTNHH467 pKa = 5.52 QLKK470 pKa = 9.54 EE471 pKa = 3.58 HH472 pKa = 6.88 AIWKK476 pKa = 9.7 EE477 pKa = 3.6 GDD479 pKa = 3.55 VQPSKK484 pKa = 11.1 DD485 pKa = 3.52 KK486 pKa = 11.22 SPCEE490 pKa = 3.8 VKK492 pKa = 10.14 HH493 pKa = 5.12 YY494 pKa = 11.12 KK495 pKa = 10.44 RR496 pKa = 11.84 MFNIPGYY503 pKa = 9.68 DD504 pKa = 3.66 DD505 pKa = 4.67 KK506 pKa = 11.45 PVVMNNCKK514 pKa = 10.35 CNEE517 pKa = 3.7 VEE519 pKa = 4.07 ALHH522 pKa = 6.62 NRR524 pKa = 11.84 YY525 pKa = 9.94 LKK527 pKa = 10.06 DD528 pKa = 2.98 TGFYY532 pKa = 10.58 QPDD535 pKa = 3.97 KK536 pKa = 10.49 IDD538 pKa = 3.12 WDD540 pKa = 4.08 IIDD543 pKa = 4.81 GLVQVLAGKK552 pKa = 9.32 LLKK555 pKa = 10.89 LSDD558 pKa = 3.94 SKK560 pKa = 11.72 NPAGLLEE567 pKa = 4.86 PNTVEE572 pKa = 4.06 EE573 pKa = 4.91 FISEE577 pKa = 4.0 KK578 pKa = 10.79 SGALKK583 pKa = 10.49 NRR585 pKa = 11.84 YY586 pKa = 8.97 CEE588 pKa = 3.82 AATNLIANGVDD599 pKa = 3.61 KK600 pKa = 11.52 NIGTVDD606 pKa = 3.4 AFIKK610 pKa = 9.93 NEE612 pKa = 4.04 KK613 pKa = 9.52 YY614 pKa = 10.71 SEE616 pKa = 4.08 IKK618 pKa = 9.16 TPRR621 pKa = 11.84 AIMGRR626 pKa = 11.84 NPQFNLLYY634 pKa = 10.24 QRR636 pKa = 11.84 YY637 pKa = 6.59 ITKK640 pKa = 10.34 AEE642 pKa = 4.17 KK643 pKa = 10.92 LFMQLPQVAKK653 pKa = 10.02 GKK655 pKa = 10.16 NFIDD659 pKa = 3.95 RR660 pKa = 11.84 GKK662 pKa = 10.23 QFSKK666 pKa = 10.83 ILGDD670 pKa = 3.96 WILEE674 pKa = 3.79 NDD676 pKa = 3.28 YY677 pKa = 11.57 SKK679 pKa = 11.53 FEE681 pKa = 4.08 GSQRR685 pKa = 11.84 TPVLMRR691 pKa = 11.84 IEE693 pKa = 3.94 YY694 pKa = 10.48 RR695 pKa = 11.84 LLCLLYY701 pKa = 10.58 GQSEE705 pKa = 4.5 DD706 pKa = 4.78 LDD708 pKa = 4.47 NIYY711 pKa = 8.19 QTKK714 pKa = 10.11 LYY716 pKa = 10.45 KK717 pKa = 10.12 KK718 pKa = 10.14 GRR720 pKa = 11.84 FPEE723 pKa = 4.4 GTKK726 pKa = 10.22 FCFKK730 pKa = 10.31 GCRR733 pKa = 11.84 GSGDD737 pKa = 3.42 MDD739 pKa = 3.48 TGFGNTVLNYY749 pKa = 9.88 IATQYY754 pKa = 10.62 FRR756 pKa = 11.84 LKK758 pKa = 8.52 NAKK761 pKa = 9.75 YY762 pKa = 9.14 VTDD765 pKa = 3.52 KK766 pKa = 11.54 DD767 pKa = 3.78 KK768 pKa = 11.27 FVVDD772 pKa = 4.61 GDD774 pKa = 4.69 DD775 pKa = 5.49 SYY777 pKa = 12.0 QQLDD781 pKa = 3.5 KK782 pKa = 11.46 GVTANKK788 pKa = 9.85 LVNTYY793 pKa = 10.22 EE794 pKa = 4.03 YY795 pKa = 10.87 FGFEE799 pKa = 4.15 TKK801 pKa = 10.83 LMIRR805 pKa = 11.84 KK806 pKa = 9.23 DD807 pKa = 3.33 PHH809 pKa = 7.7 DD810 pKa = 3.96 VEE812 pKa = 4.89 FCSSKK817 pKa = 10.32 FLEE820 pKa = 4.53 YY821 pKa = 10.68 KK822 pKa = 9.82 PGEE825 pKa = 4.13 YY826 pKa = 10.22 VQALSLKK833 pKa = 10.25 KK834 pKa = 10.39 LLNNIQYY841 pKa = 10.93 LNQTQHH847 pKa = 5.14 EE848 pKa = 4.44 HH849 pKa = 5.92 SADD852 pKa = 3.17 VYY854 pKa = 9.51 YY855 pKa = 11.16 ASLGIMYY862 pKa = 9.96 RR863 pKa = 11.84 RR864 pKa = 11.84 IYY866 pKa = 10.86 GDD868 pKa = 3.09 IPIYY872 pKa = 10.65 SHH874 pKa = 6.51 LAEE877 pKa = 4.35 YY878 pKa = 11.18 LMTASGQYY886 pKa = 9.82 SYY888 pKa = 11.67 EE889 pKa = 4.29 LIRR892 pKa = 11.84 TVNASYY898 pKa = 10.54 IDD900 pKa = 3.58 HH901 pKa = 6.73 VKK903 pKa = 10.8 DD904 pKa = 3.74 LNGDD908 pKa = 4.41 LKK910 pKa = 10.98 CDD912 pKa = 3.59 QSLTKK917 pKa = 10.65 AGITLCFGISQEE929 pKa = 4.09 EE930 pKa = 4.25 QINLIRR936 pKa = 11.84 FFQNNTLSLPEE947 pKa = 4.52 EE948 pKa = 4.14 YY949 pKa = 9.96 CHH951 pKa = 7.77 RR952 pKa = 11.84 YY953 pKa = 8.94 RR954 pKa = 11.84 APKK957 pKa = 10.19 NKK959 pKa = 9.86 YY960 pKa = 8.82 KK961 pKa = 10.9 LEE963 pKa = 4.62 NIPLSLIDD971 pKa = 4.87 SMGCVKK977 pKa = 10.32 PVIQTQGGPIPIIPEE992 pKa = 3.31 FDD994 pKa = 3.75 MIIEE998 pKa = 4.37 ATTTNAPPIVNAEE1011 pKa = 4.37 KK1012 pKa = 10.15 IDD1014 pKa = 3.72 AALKK1018 pKa = 10.17 NRR1020 pKa = 11.84 SITKK1024 pKa = 10.3 RR1025 pKa = 11.84 IRR1027 pKa = 11.84 HH1028 pKa = 5.4 LFKK1031 pKa = 10.66 RR1032 pKa = 11.84 SQPSSGDD1039 pKa = 3.2 GNVEE1043 pKa = 3.83 INEE1046 pKa = 3.86 VDD1048 pKa = 3.04
Molecular weight: 120.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.356
IPC2_protein 7.775
IPC_protein 7.556
Toseland 7.322
ProMoST 8.156
Dawson 8.346
Bjellqvist 8.609
Wikipedia 8.287
Rodwell 8.39
Grimsley 7.395
Solomon 8.375
Lehninger 8.39
Nozaki 8.726
DTASelect 8.39
Thurlkill 8.448
EMBOSS 8.536
Sillero 8.726
Patrickios 4.24
IPC_peptide 8.375
IPC2_peptide 7.41
IPC2.peptide.svr19 7.758
Protein with the highest isoelectric point:
>tr|A0A1L3KGT9|A0A1L3KGT9_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 15 OX=1923668 PE=4 SV=1
MM1 pKa = 7.48 SKK3 pKa = 10.29 QYY5 pKa = 11.17 VPVTYY10 pKa = 10.45 SRR12 pKa = 11.84 PVKK15 pKa = 10.59 SKK17 pKa = 9.85 AATIKK22 pKa = 10.65 RR23 pKa = 11.84 SGTSILVSHH32 pKa = 6.68 EE33 pKa = 4.47 EE34 pKa = 4.17 YY35 pKa = 10.49 IQDD38 pKa = 3.73 VVATTAFSCITIPINPGVQTSFPWLSLLAAGYY70 pKa = 9.41 EE71 pKa = 4.35 SYY73 pKa = 10.63 SIKK76 pKa = 10.83 SLVVEE81 pKa = 4.21 YY82 pKa = 10.38 EE83 pKa = 4.04 PRR85 pKa = 11.84 CATTTQGTVILAFDD99 pKa = 4.43 SDD101 pKa = 4.38 ASDD104 pKa = 3.68 PAPPGKK110 pKa = 9.63 VQAMQYY116 pKa = 10.03 DD117 pKa = 4.3 PNIAIPPWQRR127 pKa = 11.84 EE128 pKa = 3.92 GLRR131 pKa = 11.84 FKK133 pKa = 11.17 PEE135 pKa = 3.25 IQKK138 pKa = 10.09 ISRR141 pKa = 11.84 HH142 pKa = 4.37 FVRR145 pKa = 11.84 FGNLAANQDD154 pKa = 3.02 IKK156 pKa = 11.36 LYY158 pKa = 10.22 DD159 pKa = 3.5 VCNLFLITQGFTSAGIQVGEE179 pKa = 3.54 IHH181 pKa = 6.33 FRR183 pKa = 11.84 YY184 pKa = 10.09 VIEE187 pKa = 4.45 FQTPQIDD194 pKa = 3.76 YY195 pKa = 10.88 DD196 pKa = 4.29 GYY198 pKa = 11.67 ALQTSARR205 pKa = 11.84 ISVTPADD212 pKa = 3.38 QVLFTTGTVIAGGAPITINTGPLTLAKK239 pKa = 10.19 VAGGGSSAATLTTSNQAMVFGGPGQYY265 pKa = 10.38 YY266 pKa = 9.61 FNVLIGSTGGITITAANVGIYY287 pKa = 9.65 TLGTVSLIYY296 pKa = 10.64 FGTPGNASYY305 pKa = 11.73 VNYY308 pKa = 10.91 NFICKK313 pKa = 9.77 VNSPGDD319 pKa = 4.04 AIALVTGATSTTWTSAGSSIRR340 pKa = 11.84 VSYY343 pKa = 9.79 YY344 pKa = 10.87 AYY346 pKa = 10.77 ALNN349 pKa = 3.84
Molecular weight: 37.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.412
IPC2_protein 6.854
IPC_protein 7.073
Toseland 6.781
ProMoST 7.38
Dawson 7.658
Bjellqvist 7.6
Wikipedia 7.6
Rodwell 7.644
Grimsley 6.81
Solomon 7.775
Lehninger 7.79
Nozaki 7.805
DTASelect 7.761
Thurlkill 7.79
EMBOSS 7.907
Sillero 8.039
Patrickios 1.087
IPC_peptide 7.775
IPC2_peptide 7.205
IPC2.peptide.svr19 7.356
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1397
349
1048
698.5
78.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.228 ± 1.789
1.646 ± 0.255
5.727 ± 1.311
5.154 ± 1.311
3.865 ± 0.22
6.156 ± 1.388
2.076 ± 0.619
7.301 ± 0.659
7.015 ± 1.967
7.015 ± 0.508
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.503 ± 0.328
5.87 ± 0.946
4.868 ± 0.439
4.581 ± 0.148
4.796 ± 0.983
6.729 ± 0.951
8.017 ± 1.17
5.369 ± 1.205
0.644 ± 0.11
5.44 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here