Filimonas effusa

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Filimonas

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4638 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q1D2G2|A0A4Q1D2G2_9BACT Conjugal transfer protein TraG OS=Filimonas effusa OX=2508721 GN=ESB13_20255 PE=3 SV=1
MM1 pKa = 8.0SEE3 pKa = 4.66FLYY6 pKa = 11.04CHH8 pKa = 7.14DD9 pKa = 5.08PLDD12 pKa = 4.14EE13 pKa = 4.28EE14 pKa = 4.34VGEE17 pKa = 4.3FLLHH21 pKa = 6.74LGNPGALIKK30 pKa = 10.23IIPLEE35 pKa = 4.36DD36 pKa = 3.3EE37 pKa = 3.96EE38 pKa = 6.94AIDD41 pKa = 4.43SEE43 pKa = 4.46EE44 pKa = 4.4FIHH47 pKa = 7.6KK48 pKa = 10.13IFIYY52 pKa = 10.38EE53 pKa = 4.09NEE55 pKa = 4.62DD56 pKa = 3.35GDD58 pKa = 4.28SEE60 pKa = 4.9SYY62 pKa = 10.94QLIFTPLATAMEE74 pKa = 4.94SPASPSSEE82 pKa = 3.88RR83 pKa = 11.84IHH85 pKa = 6.63EE86 pKa = 4.32VLEE89 pKa = 4.5SAWEE93 pKa = 3.91YY94 pKa = 7.02WTEE97 pKa = 3.9VLNWEE102 pKa = 4.2EE103 pKa = 5.45DD104 pKa = 3.71EE105 pKa = 5.42DD106 pKa = 5.4DD107 pKa = 4.2EE108 pKa = 5.11EE109 pKa = 4.43

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q1DBR8|A0A4Q1DBR8_9BACT Uncharacterized protein OS=Filimonas effusa OX=2508721 GN=ESB13_08885 PE=4 SV=1
MM1 pKa = 7.76AKK3 pKa = 10.02QGGPVILEE11 pKa = 3.83KK12 pKa = 10.48TIGIVSCYY20 pKa = 9.27KK21 pKa = 9.99RR22 pKa = 11.84CGVGCVRR29 pKa = 11.84MKK31 pKa = 11.2SNLTRR36 pKa = 11.84EE37 pKa = 4.11RR38 pKa = 11.84WQTDD42 pKa = 3.18PLFAGSRR49 pKa = 11.84KK50 pKa = 9.35SAAHH54 pKa = 6.6LAAASVLASKK64 pKa = 10.04YY65 pKa = 9.57YY66 pKa = 10.08RR67 pKa = 11.84VIPEE71 pKa = 3.48QHH73 pKa = 5.52RR74 pKa = 11.84VVPHH78 pKa = 5.67FRR80 pKa = 11.84QLVGIAKK87 pKa = 9.72EE88 pKa = 3.88MLSAGFSLQQINKK101 pKa = 8.07VLDD104 pKa = 3.5YY105 pKa = 8.74TVCRR109 pKa = 11.84FIRR112 pKa = 11.84KK113 pKa = 8.3MEE115 pKa = 4.1AANRR119 pKa = 11.84RR120 pKa = 11.84GKK122 pKa = 9.44PVRR125 pKa = 11.84SSFAAKK131 pKa = 10.21SSLPPKK137 pKa = 9.87PAFTRR142 pKa = 11.84PVFAGLTIAQRR153 pKa = 11.84PVLTALHH160 pKa = 6.52LPDD163 pKa = 3.99VVLPAIDD170 pKa = 4.03KK171 pKa = 9.86PPRR174 pKa = 11.84GQPVEE179 pKa = 4.07TMPVKK184 pKa = 10.45NRR186 pKa = 11.84QLILFGTRR194 pKa = 11.84TAAAAGFVMPRR205 pKa = 11.84SEE207 pKa = 4.34INIISCMNN215 pKa = 3.65

Molecular weight: 23.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4638

0

4638

1792117

26

5165

386.4

43.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.35 ± 0.039

0.863 ± 0.013

5.019 ± 0.021

5.379 ± 0.033

4.609 ± 0.023

7.064 ± 0.043

1.892 ± 0.018

6.464 ± 0.025

6.215 ± 0.034

9.409 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.017

5.392 ± 0.04

4.048 ± 0.019

4.022 ± 0.024

4.462 ± 0.024

6.447 ± 0.034

5.97 ± 0.042

6.551 ± 0.028

1.307 ± 0.013

4.255 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski