Vibrio phage Chazly21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; Cetovirus; unclassified Cetovirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XYS2|A0A6G6XYS2_9CAUD Uncharacterized protein OS=Vibrio phage Chazly21 OX=2712960 GN=CHAZLY21_196 PE=4 SV=1
MM1 pKa = 7.75ADD3 pKa = 3.22FWDD6 pKa = 3.31KK7 pKa = 11.11HH8 pKa = 5.58LNKK11 pKa = 9.27VQQYY15 pKa = 9.68EE16 pKa = 4.25IVDD19 pKa = 4.2SVAGTCEE26 pKa = 3.93SGCNVDD32 pKa = 3.16YY33 pKa = 9.61WQKK36 pKa = 10.85EE37 pKa = 3.94FGFSDD42 pKa = 3.81EE43 pKa = 5.19VRR45 pKa = 11.84DD46 pKa = 3.74EE47 pKa = 4.45LDD49 pKa = 3.18WNFGEE54 pKa = 4.57LAAKK58 pKa = 10.46FEE60 pKa = 4.5LFRR63 pKa = 11.84CEE65 pKa = 4.21DD66 pKa = 3.94CQWWGDD72 pKa = 3.48MGEE75 pKa = 4.02VGEE78 pKa = 4.63CNEE81 pKa = 4.67CGGDD85 pKa = 3.81LVV87 pKa = 4.4

Molecular weight: 10.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XZG1|A0A6G6XZG1_9CAUD D3 protein OS=Vibrio phage Chazly21 OX=2712960 GN=CHAZLY21_65 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.23RR4 pKa = 11.84SKK6 pKa = 10.37MIGIKK11 pKa = 9.91MSDD14 pKa = 3.72RR15 pKa = 11.84TRR17 pKa = 11.84KK18 pKa = 8.26MAGEE22 pKa = 3.94YY23 pKa = 10.34GIEE26 pKa = 3.78ILEE29 pKa = 4.0QRR31 pKa = 11.84RR32 pKa = 11.84SGLAKK37 pKa = 10.62VLGLLARR44 pKa = 11.84GTRR47 pKa = 11.84VSNHH51 pKa = 5.19PRR53 pKa = 11.84AVEE56 pKa = 3.66YY57 pKa = 9.12TAAQFGLL64 pKa = 3.85

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

180

0

180

35761

35

1018

198.7

22.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.796 ± 0.308

1.214 ± 0.108

6.283 ± 0.153

6.996 ± 0.2

3.968 ± 0.141

6.527 ± 0.196

2.117 ± 0.112

6.138 ± 0.159

7.153 ± 0.191

8.316 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.134

5.159 ± 0.127

3.462 ± 0.137

3.498 ± 0.128

4.46 ± 0.146

6.183 ± 0.308

6.141 ± 0.201

6.566 ± 0.153

1.311 ± 0.067

4.002 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski