Lactobacillus phage KC5a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q20DB7|Q20DB7_9CAUD Uncharacterized protein orf56 OS=Lactobacillus phage KC5a OX=363555 GN=orf56 PE=4 SV=1
MM1 pKa = 7.74FDD3 pKa = 4.24FDD5 pKa = 4.79FSSIYY10 pKa = 10.98SNLEE14 pKa = 3.97SLCKK18 pKa = 10.25SSLDD22 pKa = 3.29NGYY25 pKa = 8.06FTDD28 pKa = 3.58STIAGFVEE36 pKa = 4.4QGTFDD41 pKa = 3.26AAGYY45 pKa = 10.18KK46 pKa = 10.18RR47 pKa = 11.84ITGDD51 pKa = 3.76DD52 pKa = 3.79YY53 pKa = 11.96VAGNQTTVANSQAA66 pKa = 3.28

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q20DE2|Q20DE2_9CAUD Uncharacterized protein orf31 OS=Lactobacillus phage KC5a OX=363555 GN=orf31 PE=4 SV=1
MM1 pKa = 7.05MGKK4 pKa = 9.8IINFIMTSRR13 pKa = 11.84KK14 pKa = 9.63ASSFFMKK21 pKa = 10.75AFMKK25 pKa = 10.35FLAVVLKK32 pKa = 9.19YY33 pKa = 10.74LPLLDD38 pKa = 4.66LVSLNLYY45 pKa = 9.87SSQRR49 pKa = 11.84INPIISKK56 pKa = 10.49LIFLL60 pKa = 4.9

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

11619

28

1184

190.5

21.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.445 ± 0.776

0.697 ± 0.122

7.006 ± 0.308

6.059 ± 0.471

3.52 ± 0.172

6.412 ± 0.578

1.274 ± 0.156

6.558 ± 0.295

8.555 ± 0.457

8.486 ± 0.401

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.286

6.154 ± 0.406

3.064 ± 0.227

3.864 ± 0.193

3.95 ± 0.342

6.137 ± 0.228

6.197 ± 0.387

6.748 ± 0.382

1.257 ± 0.152

3.976 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski