Bacillus phage Troll

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Bequatrovirus; Bacillus virus Troll

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 289 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5Z7L9|S5Z7L9_9CAUD Uncharacterized protein OS=Bacillus phage Troll OX=1382932 GN=14 PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 4.75EE3 pKa = 3.86VNLKK7 pKa = 9.2HH8 pKa = 6.65RR9 pKa = 11.84YY10 pKa = 6.76WVFDD14 pKa = 3.15IPEE17 pKa = 4.74FYY19 pKa = 8.69PTGGLSDD26 pKa = 3.09ITYY29 pKa = 6.75TTNSVVYY36 pKa = 9.97ALIKK40 pKa = 10.54IEE42 pKa = 5.23DD43 pKa = 4.46DD44 pKa = 3.54PDD46 pKa = 6.38LILFDD51 pKa = 4.83SITRR55 pKa = 11.84LYY57 pKa = 10.17YY58 pKa = 9.96WEE60 pKa = 5.58EE61 pKa = 3.97DD62 pKa = 3.93DD63 pKa = 3.86EE64 pKa = 4.45WRR66 pKa = 4.81

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5Y728|S5Y728_9CAUD HTH binding domain protein OS=Bacillus phage Troll OX=1382932 GN=72 PE=4 SV=1
MM1 pKa = 7.21NVNRR5 pKa = 11.84FYY7 pKa = 10.6IIHH10 pKa = 6.16EE11 pKa = 4.05LARR14 pKa = 11.84VKK16 pKa = 10.46LHH18 pKa = 6.15YY19 pKa = 10.69GGIKK23 pKa = 9.31QRR25 pKa = 11.84HH26 pKa = 5.11LEE28 pKa = 3.83RR29 pKa = 11.84RR30 pKa = 11.84AQQLEE35 pKa = 4.43TTPKK39 pKa = 10.49NLMIAVNRR47 pKa = 11.84YY48 pKa = 4.9ITKK51 pKa = 10.33HH52 pKa = 5.45NGIII56 pKa = 3.6

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

289

0

289

50165

31

2035

173.6

19.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.731 ± 0.226

0.889 ± 0.076

6.365 ± 0.131

8.464 ± 0.327

3.943 ± 0.092

6.333 ± 0.228

1.832 ± 0.1

6.574 ± 0.132

8.368 ± 0.203

8.099 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.09

5.546 ± 0.168

2.89 ± 0.121

3.369 ± 0.134

4.152 ± 0.124

5.462 ± 0.162

6.144 ± 0.229

6.969 ± 0.176

1.182 ± 0.059

4.79 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski