Salinicoccus roseus
Average proteome isoelectric point is 5.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2412 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C2HG02|A0A0C2HG02_9STAP Transporter OS=Salinicoccus roseus OX=45670 GN=SN16_07605 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 10.34 RR3 pKa = 11.84 FIILLSIPMFITACTNPDD21 pKa = 3.55 DD22 pKa = 4.1 EE23 pKa = 4.74 TAEE26 pKa = 4.22 SEE28 pKa = 4.15 PSEE31 pKa = 4.27 EE32 pKa = 4.09 EE33 pKa = 3.88 TTEE36 pKa = 4.06 EE37 pKa = 3.81 EE38 pKa = 4.43 TEE40 pKa = 4.15 EE41 pKa = 4.06 VTEE44 pKa = 4.24 EE45 pKa = 4.06 EE46 pKa = 4.73 TEE48 pKa = 4.18 EE49 pKa = 3.95 EE50 pKa = 4.39 APPEE54 pKa = 3.95 VTPIDD59 pKa = 3.74 VTSDD63 pKa = 3.0 AFISNFFSGNYY74 pKa = 8.26 EE75 pKa = 3.73 YY76 pKa = 10.09 RR77 pKa = 11.84 TISVHH82 pKa = 6.21 SSYY85 pKa = 11.42 DD86 pKa = 3.46 YY87 pKa = 10.9 MVDD90 pKa = 3.9 RR91 pKa = 11.84 LGDD94 pKa = 3.61 PAGSGSTVDD103 pKa = 3.15 GTYY106 pKa = 10.61 YY107 pKa = 10.54 HH108 pKa = 7.03 YY109 pKa = 11.55 DD110 pKa = 3.31 HH111 pKa = 7.29 IGFNFPEE118 pKa = 4.36 AVGSVEE124 pKa = 4.23 DD125 pKa = 3.9 TSEE128 pKa = 4.29 LKK130 pKa = 10.66 VDD132 pKa = 4.63 GIIIFPEE139 pKa = 4.16 EE140 pKa = 4.16 FSKK143 pKa = 11.04 QDD145 pKa = 3.39 AVEE148 pKa = 4.11 HH149 pKa = 6.07 FGWPSSDD156 pKa = 3.22 EE157 pKa = 4.11 VSDD160 pKa = 3.85 FRR162 pKa = 11.84 MFYY165 pKa = 10.91 DD166 pKa = 3.74 SDD168 pKa = 4.03 DD169 pKa = 4.35 SNGQYY174 pKa = 11.93 VMMKK178 pKa = 9.92 YY179 pKa = 9.83 SQEE182 pKa = 3.83 DD183 pKa = 4.29 RR184 pKa = 11.84 ITEE187 pKa = 4.36 IILQYY192 pKa = 10.94 KK193 pKa = 9.75 DD194 pKa = 4.19 LSDD197 pKa = 3.67 TEE199 pKa = 5.33 FYY201 pKa = 11.03 DD202 pKa = 3.74
Molecular weight: 23.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.541
ProMoST 3.846
Dawson 3.706
Bjellqvist 3.884
Wikipedia 3.605
Rodwell 3.567
Grimsley 3.452
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.859
Patrickios 0.871
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|A0A0C2HLR2|A0A0C2HLR2_9STAP Cardiolipin synthase OS=Salinicoccus roseus OX=45670 GN=SN16_07090 PE=3 SV=1
MM1 pKa = 6.38 TTVLRR6 pKa = 11.84 RR7 pKa = 11.84 ITFVIVIIAIWEE19 pKa = 4.5 VISKK23 pKa = 9.72 LQFFPTFMFPPLIIPNDD40 pKa = 3.96 PGGVTVIKK48 pKa = 9.63 TLITGILSGQILEE61 pKa = 4.39 ATGITLGRR69 pKa = 11.84 LLIGFFIAVILGLTFGFLIARR90 pKa = 11.84 YY91 pKa = 8.93 KK92 pKa = 10.09 WVDD95 pKa = 3.15 DD96 pKa = 3.6 TLGFFVTALQSIPSIVWFPLAIVWFGLGNVAILFIVAIGATWTMTVNSSAGFKK149 pKa = 9.84 NVPSIYY155 pKa = 9.43 IDD157 pKa = 3.27 AARR160 pKa = 11.84 TLGASGTHH168 pKa = 6.19 LVRR171 pKa = 11.84 TVIIPASVPHH181 pKa = 7.14 IISGLRR187 pKa = 11.84 VAWAFAWRR195 pKa = 11.84 AIMAGEE201 pKa = 4.11 LLGAAGGLGHH211 pKa = 7.58 LLDD214 pKa = 5.24 LGRR217 pKa = 11.84 SIQAMDD223 pKa = 4.16 LVLSIMIVIGIIGTIIDD240 pKa = 3.68 NQVFLRR246 pKa = 11.84 MEE248 pKa = 4.29 RR249 pKa = 11.84 SVSRR253 pKa = 11.84 RR254 pKa = 11.84 WGLGTT259 pKa = 4.02
Molecular weight: 28.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.56
IPC_protein 10.496
Toseland 10.701
ProMoST 10.452
Dawson 10.774
Bjellqvist 10.511
Wikipedia 11.023
Rodwell 10.877
Grimsley 10.818
Solomon 10.95
Lehninger 10.906
Nozaki 10.657
DTASelect 10.511
Thurlkill 10.687
EMBOSS 11.111
Sillero 10.716
Patrickios 10.657
IPC_peptide 10.95
IPC2_peptide 9.341
IPC2.peptide.svr19 8.77
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2412
0
2412
735775
37
1489
305.0
34.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.344 ± 0.051
0.548 ± 0.013
6.113 ± 0.044
8.169 ± 0.058
4.391 ± 0.035
7.364 ± 0.045
2.322 ± 0.027
7.64 ± 0.045
5.575 ± 0.049
9.245 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.29 ± 0.022
4.003 ± 0.033
3.523 ± 0.024
3.075 ± 0.026
4.652 ± 0.044
5.828 ± 0.033
5.372 ± 0.03
7.126 ± 0.039
0.831 ± 0.019
3.589 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here