Rhodococcus sp. 1168

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4386 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X0UQA7|A0A1X0UQA7_9NOCA Uncharacterized protein OS=Rhodococcus sp. 1168 OX=2018041 GN=BJI47_18360 PE=4 SV=1
MM1 pKa = 7.0KK2 pKa = 9.54TLVHH6 pKa = 6.37AAIAAAVVLALATGCSDD23 pKa = 3.98DD24 pKa = 4.27GSVGAAATSTVDD36 pKa = 3.2AVKK39 pKa = 10.53SQTSAPTRR47 pKa = 11.84VSGSVGASARR57 pKa = 11.84PGEE60 pKa = 4.63SGAPATPDD68 pKa = 3.1APLPPTPVDD77 pKa = 3.44NPTDD81 pKa = 3.67PSAAVPVDD89 pKa = 3.27QRR91 pKa = 11.84NPCLDD96 pKa = 3.37TSSDD100 pKa = 3.38LVQNVIATLGPPPGSDD116 pKa = 3.06FYY118 pKa = 11.4EE119 pKa = 5.41IGEE122 pKa = 4.43HH123 pKa = 4.84TTATTPNCPTLTWAVAEE140 pKa = 4.61TPSGTGSSPEE150 pKa = 4.15SIMFFHH156 pKa = 7.82DD157 pKa = 3.25NMFARR162 pKa = 11.84LATPSPSAFTSISGSTDD179 pKa = 3.06DD180 pKa = 4.59SVTVHH185 pKa = 5.79YY186 pKa = 10.23SWLVGDD192 pKa = 5.19EE193 pKa = 4.66PNCCPQGNADD203 pKa = 4.22VTFSWDD209 pKa = 3.04GSNVVSDD216 pKa = 3.95VPVPTEE222 pKa = 3.84VYY224 pKa = 10.9NFGG227 pKa = 3.7

Molecular weight: 23.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X0UL73|A0A1X0UL73_9NOCA Mutator family transposase OS=Rhodococcus sp. 1168 OX=2018041 GN=BJI47_00130 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.01GRR42 pKa = 11.84KK43 pKa = 8.43EE44 pKa = 3.47LTAA47 pKa = 4.94

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4386

0

4386

1423437

32

5246

324.5

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.172 ± 0.051

0.713 ± 0.01

6.332 ± 0.031

5.648 ± 0.033

3.095 ± 0.022

8.659 ± 0.039

2.085 ± 0.017

4.68 ± 0.025

2.489 ± 0.027

9.91 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.979 ± 0.014

2.256 ± 0.017

5.243 ± 0.03

2.817 ± 0.019

6.867 ± 0.031

6.491 ± 0.03

6.263 ± 0.028

8.843 ± 0.039

1.412 ± 0.015

2.045 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski