Shahe isopoda virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG25|A0A1L3KG25_9VIRU Uncharacterized protein OS=Shahe isopoda virus 5 OX=1923425 PE=4 SV=1
MM1 pKa = 7.41SLALLSLQDD10 pKa = 3.7PTFVAYY16 pKa = 9.53HH17 pKa = 6.63LCLISWDD24 pKa = 3.52GDD26 pKa = 3.57EE27 pKa = 4.07QGEE30 pKa = 4.57YY31 pKa = 10.96NFWEE35 pKa = 4.22WKK37 pKa = 9.49KK38 pKa = 10.81VYY40 pKa = 10.32GLEE43 pKa = 4.0KK44 pKa = 10.39HH45 pKa = 5.27VTAEE49 pKa = 4.26MVCDD53 pKa = 3.81ALEE56 pKa = 4.66GPGPQGEE63 pKa = 4.42ANEE66 pKa = 4.25EE67 pKa = 4.13SSALFGMQQIASFPAIRR84 pKa = 11.84AGLLEE89 pKa = 4.63KK90 pKa = 10.1KK91 pKa = 9.21WKK93 pKa = 9.38EE94 pKa = 3.76AGLAPPRR101 pKa = 11.84YY102 pKa = 8.13WHH104 pKa = 6.76LLTRR108 pKa = 11.84GGQQRR113 pKa = 11.84IITLTRR119 pKa = 11.84AIQEE123 pKa = 4.14RR124 pKa = 11.84FGTEE128 pKa = 3.05WRR130 pKa = 11.84GCSGTWASIGATFTLRR146 pKa = 11.84RR147 pKa = 11.84AEE149 pKa = 4.08QASQQLTVGDD159 pKa = 5.03LDD161 pKa = 4.31PEE163 pKa = 4.69STCLLWRR170 pKa = 11.84RR171 pKa = 11.84DD172 pKa = 3.06WQEE175 pKa = 4.09NYY177 pKa = 8.54WQVEE181 pKa = 4.1LFGPQPLGNRR191 pKa = 11.84AGGCRR196 pKa = 11.84FYY198 pKa = 11.74VEE200 pKa = 5.22GNTALQPGGAGASAAPTRR218 pKa = 11.84KK219 pKa = 9.42ARR221 pKa = 11.84RR222 pKa = 11.84GGKK225 pKa = 7.27EE226 pKa = 2.95ARR228 pKa = 11.84ARR230 pKa = 11.84HH231 pKa = 4.6NAKK234 pKa = 10.23RR235 pKa = 11.84FAEE238 pKa = 4.15KK239 pKa = 10.72LLLLEE244 pKa = 4.44SRR246 pKa = 11.84GEE248 pKa = 3.99LGSWANGWVFDD259 pKa = 4.19IPDD262 pKa = 3.89PVRR265 pKa = 11.84DD266 pKa = 3.37RR267 pKa = 11.84ARR269 pKa = 11.84QYY271 pKa = 11.0LPPDD275 pKa = 3.54FVIPP279 pKa = 4.07

Molecular weight: 31.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG48|A0A1L3KG48_9VIRU RNA-directed RNA polymerase OS=Shahe isopoda virus 5 OX=1923425 PE=4 SV=1
MM1 pKa = 7.74AMNRR5 pKa = 11.84VNTTSGSGRR14 pKa = 11.84KK15 pKa = 8.12STVSRR20 pKa = 11.84STSRR24 pKa = 11.84PRR26 pKa = 11.84WSVMLSKK33 pKa = 11.07GPALKK38 pKa = 10.03EE39 pKa = 3.53RR40 pKa = 11.84RR41 pKa = 11.84TRR43 pKa = 11.84RR44 pKa = 11.84VQHH47 pKa = 6.47CSGCNRR53 pKa = 11.84SHH55 pKa = 7.4PFLPFEE61 pKa = 4.55PDD63 pKa = 2.89CLRR66 pKa = 11.84RR67 pKa = 11.84SGRR70 pKa = 11.84RR71 pKa = 11.84QDD73 pKa = 4.35LPPHH77 pKa = 5.79ATGTSLRR84 pKa = 11.84EE85 pKa = 3.72VASRR89 pKa = 11.84GSSRR93 pKa = 11.84SLGQSRR99 pKa = 11.84RR100 pKa = 11.84DD101 pKa = 3.5SVRR104 pKa = 11.84SGGVAAARR112 pKa = 11.84GPVLGQHH119 pKa = 5.94SRR121 pKa = 11.84SDD123 pKa = 3.99GLNKK127 pKa = 10.07LHH129 pKa = 6.69NSSRR133 pKa = 11.84SATSTQSLPAFSGEE147 pKa = 4.0GTGRR151 pKa = 11.84RR152 pKa = 11.84TTGRR156 pKa = 11.84LSSSGPSPWEE166 pKa = 3.8TEE168 pKa = 3.92RR169 pKa = 11.84VGAGFMWRR177 pKa = 11.84EE178 pKa = 4.07TPPSSRR184 pKa = 11.84VEE186 pKa = 4.0LGQALPQPAKK196 pKa = 10.49QGGEE200 pKa = 3.88EE201 pKa = 3.96KK202 pKa = 10.4RR203 pKa = 11.84RR204 pKa = 11.84ARR206 pKa = 11.84ATMPSGSRR214 pKa = 11.84KK215 pKa = 9.97SSFCSSRR222 pKa = 11.84EE223 pKa = 3.45EE224 pKa = 4.14SLAVGQTDD232 pKa = 3.98GSSTSPIQSVTEE244 pKa = 3.93QGSISPPISSSRR256 pKa = 11.84KK257 pKa = 7.23GGAYY261 pKa = 9.85AGPSRR266 pKa = 11.84GEE268 pKa = 4.13GPAPEE273 pKa = 4.32HH274 pKa = 6.06EE275 pKa = 4.71KK276 pKa = 10.45PVKK279 pKa = 10.27AKK281 pKa = 10.38CDD283 pKa = 3.42EE284 pKa = 4.28TFVPDD289 pKa = 3.23RR290 pKa = 11.84RR291 pKa = 11.84VRR293 pKa = 11.84ILGNKK298 pKa = 8.58NLVGRR303 pKa = 11.84GARR306 pKa = 11.84KK307 pKa = 9.7KK308 pKa = 9.81IRR310 pKa = 11.84RR311 pKa = 11.84EE312 pKa = 4.06VPCVDD317 pKa = 5.22DD318 pKa = 4.3EE319 pKa = 5.0LLGHH323 pKa = 7.14LLRR326 pKa = 11.84KK327 pKa = 9.16FAFVPRR333 pKa = 11.84TAEE336 pKa = 3.78LMVVMSRR343 pKa = 11.84TAQAFYY349 pKa = 10.85KK350 pKa = 10.82GFDD353 pKa = 3.38LSEE356 pKa = 3.89WTEE359 pKa = 4.0LEE361 pKa = 4.24LYY363 pKa = 7.86KK364 pKa = 8.86TTVMTITAAMDD375 pKa = 3.41IPDD378 pKa = 3.89EE379 pKa = 4.35EE380 pKa = 4.56EE381 pKa = 3.8ACRR384 pKa = 11.84QHH386 pKa = 6.85LKK388 pKa = 10.69SKK390 pKa = 10.67SLGEE394 pKa = 3.88ARR396 pKa = 11.84EE397 pKa = 4.11AHH399 pKa = 5.61AKK401 pKa = 8.21MMKK404 pKa = 9.96GHH406 pKa = 7.57LGNVRR411 pKa = 11.84NIFGVGRR418 pKa = 11.84TAVLPGKK425 pKa = 10.75GSGXDD430 pKa = 3.44RR431 pKa = 11.84LRR433 pKa = 11.84ITAHH437 pKa = 5.67CQRR440 pKa = 11.84EE441 pKa = 3.87VDD443 pKa = 3.81LTNNRR448 pKa = 11.84LPGCVSRR455 pKa = 11.84AGPQPCATKK464 pKa = 10.41RR465 pKa = 11.84STSRR469 pKa = 11.84LVSPVEE475 pKa = 3.65GWEE478 pKa = 4.31GVIVTHH484 pKa = 6.46SDD486 pKa = 3.78CVCNEE491 pKa = 3.26MRR493 pKa = 11.84ALHH496 pKa = 6.37HH497 pKa = 6.43RR498 pKa = 11.84HH499 pKa = 5.69QKK501 pKa = 7.58TAPAPTRR508 pKa = 11.84EE509 pKa = 4.03GLAMLKK515 pKa = 10.38KK516 pKa = 9.89KK517 pKa = 9.16VHH519 pKa = 5.79EE520 pKa = 4.26LFVVEE525 pKa = 4.72GVPGVIPKK533 pKa = 9.68PRR535 pKa = 11.84EE536 pKa = 3.82AVLEE540 pKa = 4.5HH541 pKa = 6.04YY542 pKa = 10.32SGRR545 pKa = 11.84QLAEE549 pKa = 3.81FTRR552 pKa = 11.84ALRR555 pKa = 11.84SLGEE559 pKa = 4.13RR560 pKa = 11.84PVCARR565 pKa = 11.84DD566 pKa = 3.34ADD568 pKa = 3.87VKK570 pKa = 10.9MFLKK574 pKa = 10.38DD575 pKa = 3.43DD576 pKa = 4.08KK577 pKa = 11.19YY578 pKa = 10.74VPEE581 pKa = 4.09YY582 pKa = 10.94AQIKK586 pKa = 9.77APRR589 pKa = 11.84CIQYY593 pKa = 10.08RR594 pKa = 11.84DD595 pKa = 3.1KK596 pKa = 10.97RR597 pKa = 11.84YY598 pKa = 9.53CLEE601 pKa = 3.92LARR604 pKa = 11.84YY605 pKa = 7.96LQIIEE610 pKa = 3.9GRR612 pKa = 11.84VYY614 pKa = 10.54GAEE617 pKa = 3.89DD618 pKa = 3.57AFGHH622 pKa = 6.12RR623 pKa = 11.84LIAKK627 pKa = 8.53GRR629 pKa = 11.84NLAQRR634 pKa = 11.84GADD637 pKa = 3.17LWAKK641 pKa = 10.47ANEE644 pKa = 4.21FADD647 pKa = 4.08PLFLLLDD654 pKa = 4.15ASNFDD659 pKa = 3.29AHH661 pKa = 6.56VATGLLKK668 pKa = 10.0IEE670 pKa = 4.14HH671 pKa = 5.8STYY674 pKa = 10.66IKK676 pKa = 9.43NTKK679 pKa = 9.69KK680 pKa = 10.39SARR683 pKa = 11.84HH684 pKa = 4.26MLRR687 pKa = 11.84WLLKK691 pKa = 8.74QQLINRR697 pKa = 11.84GRR699 pKa = 11.84TKK701 pKa = 10.94NGTTYY706 pKa = 9.14LTPGTRR712 pKa = 11.84MSGDD716 pKa = 3.36MNTGLGNSILMAGMLEE732 pKa = 4.72CYY734 pKa = 10.12LEE736 pKa = 4.13KK737 pKa = 10.79CGIKK741 pKa = 10.31GAVYY745 pKa = 10.76VDD747 pKa = 4.03GDD749 pKa = 3.83DD750 pKa = 3.56SVVVVEE756 pKa = 5.08RR757 pKa = 11.84QQQHH761 pKa = 6.0KK762 pKa = 9.22LLPVAPFFLQFGMEE776 pKa = 4.15MKK778 pKa = 10.87YY779 pKa = 10.17EE780 pKa = 4.05ATNEE784 pKa = 3.58FSQVDD789 pKa = 4.04FCQCRR794 pKa = 11.84PVQVDD799 pKa = 3.76GKK801 pKa = 9.14WVLSRR806 pKa = 11.84DD807 pKa = 3.7PKK809 pKa = 10.67RR810 pKa = 11.84VLTRR814 pKa = 11.84PLWTTRR820 pKa = 11.84EE821 pKa = 4.11MGDD824 pKa = 3.48KK825 pKa = 10.75LAGRR829 pKa = 11.84YY830 pKa = 9.16LKK832 pKa = 10.93GLGLGEE838 pKa = 4.24IAVNWGLPLGSVLGARR854 pKa = 11.84LYY856 pKa = 10.7EE857 pKa = 4.74IGEE860 pKa = 4.28GKK862 pKa = 8.35PWSYY866 pKa = 11.01EE867 pKa = 3.47FHH869 pKa = 7.22PGMKK873 pKa = 9.6ARR875 pKa = 11.84EE876 pKa = 4.18YY877 pKa = 11.42GRR879 pKa = 11.84IDD881 pKa = 3.35TPQPSWATRR890 pKa = 11.84MSFFEE895 pKa = 3.83AWGISPEE902 pKa = 3.98EE903 pKa = 3.73QEE905 pKa = 4.42AIEE908 pKa = 3.9RR909 pKa = 11.84SIRR912 pKa = 11.84SIHH915 pKa = 5.8RR916 pKa = 11.84TPTLPQDD923 pKa = 3.95LLEE926 pKa = 5.12GDD928 pKa = 3.84TLGPGFDD935 pKa = 3.5RR936 pKa = 11.84RR937 pKa = 3.77

Molecular weight: 103.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1765

149

937

441.3

48.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.215 ± 0.832

1.7 ± 0.265

3.739 ± 0.337

5.949 ± 1.054

3.286 ± 0.561

9.292 ± 0.567

1.813 ± 0.597

3.626 ± 0.552

4.816 ± 0.505

8.952 ± 1.62

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.348

2.89 ± 0.596

6.516 ± 0.638

4.306 ± 0.603

8.272 ± 1.488

7.875 ± 1.071

6.346 ± 0.861

5.722 ± 0.501

1.7 ± 0.618

2.436 ± 0.603
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski