Drosophila subobscura Nora virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075E1R9|A0A075E1R9_9VIRU VP2 OS=Drosophila subobscura Nora virus OX=1500865 GN=VP2 PE=4 SV=1
MM1 pKa = 7.49ALKK4 pKa = 10.69EE5 pKa = 4.29EE6 pKa = 4.59IFDD9 pKa = 3.82QNTTLFSVLDD19 pKa = 4.05EE20 pKa = 4.64NEE22 pKa = 3.98VTEE25 pKa = 4.5TKK27 pKa = 10.4QIQNSVTTVQTQIDD41 pKa = 3.98QQKK44 pKa = 9.99LQLDD48 pKa = 4.31GLAKK52 pKa = 10.62VVDD55 pKa = 4.11QNQSRR60 pKa = 11.84NEE62 pKa = 3.87EE63 pKa = 3.82QFVNINTIIVGMNDD77 pKa = 5.1DD78 pKa = 3.78IDD80 pKa = 3.87KK81 pKa = 11.43LKK83 pKa = 9.45ITTTNLVQQTNSLNSSVAEE102 pKa = 4.11LEE104 pKa = 4.39TFVPRR109 pKa = 11.84VNSLEE114 pKa = 4.12VQTDD118 pKa = 3.42ANTKK122 pKa = 10.31SISALEE128 pKa = 4.1GTTDD132 pKa = 5.46GIQDD136 pKa = 4.41SINTINDD143 pKa = 4.51GIVEE147 pKa = 4.29LKK149 pKa = 10.63DD150 pKa = 3.68DD151 pKa = 4.37NKK153 pKa = 11.18DD154 pKa = 3.34LSSQIQALDD163 pKa = 3.32SRR165 pKa = 11.84ITILEE170 pKa = 4.11SKK172 pKa = 9.7IKK174 pKa = 10.63SSFIEE179 pKa = 4.01KK180 pKa = 9.9SVQYY184 pKa = 10.3KK185 pKa = 10.23ISYY188 pKa = 9.74SNNSDD193 pKa = 3.29VQRR196 pKa = 11.84VLNFYY201 pKa = 11.14ALGPVKK207 pKa = 10.35YY208 pKa = 8.78GQSFSVMQTPTSTFSTTLTINRR230 pKa = 11.84PYY232 pKa = 9.62PQLSAITYY240 pKa = 8.04PGVPVLIGMQVSLSGTYY257 pKa = 10.72PMGTNVFEE265 pKa = 4.35SLKK268 pKa = 10.46DD269 pKa = 3.56RR270 pKa = 11.84PAIIYY275 pKa = 9.95SEE277 pKa = 4.13QQ278 pKa = 3.08

Molecular weight: 30.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075E1L1|A0A075E1L1_9VIRU VP3 OS=Drosophila subobscura Nora virus OX=1500865 GN=VP3 PE=4 SV=1
MM1 pKa = 7.18STNKK5 pKa = 9.05TNKK8 pKa = 9.71KK9 pKa = 8.79GAQLEE14 pKa = 4.25RR15 pKa = 11.84VQIASTQVVDD25 pKa = 3.63TRR27 pKa = 11.84SKK29 pKa = 10.05RR30 pKa = 11.84RR31 pKa = 11.84QRR33 pKa = 11.84GTKK36 pKa = 9.92LDD38 pKa = 2.98IDD40 pKa = 4.06YY41 pKa = 9.18TVKK44 pKa = 10.71KK45 pKa = 10.54NNAEE49 pKa = 4.08KK50 pKa = 10.15EE51 pKa = 4.33QKK53 pKa = 10.54FLDD56 pKa = 3.97TEE58 pKa = 4.45VVDD61 pKa = 4.16TKK63 pKa = 11.09LDD65 pKa = 3.59KK66 pKa = 11.04QILYY70 pKa = 9.62EE71 pKa = 3.94KK72 pKa = 9.68RR73 pKa = 11.84QNHH76 pKa = 5.25TFIKK80 pKa = 9.66PKK82 pKa = 10.17LSMVSRR88 pKa = 11.84EE89 pKa = 4.03DD90 pKa = 3.85KK91 pKa = 10.35IVKK94 pKa = 10.32NKK96 pKa = 8.86VLRR99 pKa = 11.84GNEE102 pKa = 3.57RR103 pKa = 11.84ASAYY107 pKa = 10.06KK108 pKa = 10.25FMKK111 pKa = 10.5EE112 pKa = 3.76MVNSNKK118 pKa = 9.24VQSSWNVEE126 pKa = 3.87RR127 pKa = 11.84VKK129 pKa = 10.93EE130 pKa = 3.96VDD132 pKa = 3.36EE133 pKa = 4.3VDD135 pKa = 3.57LFFRR139 pKa = 11.84KK140 pKa = 9.83KK141 pKa = 8.26KK142 pKa = 8.58TKK144 pKa = 9.41PFSGFSIGEE153 pKa = 4.07LRR155 pKa = 11.84DD156 pKa = 3.76SLIVPSDD163 pKa = 3.89DD164 pKa = 3.86KK165 pKa = 11.67NIVPPIVMSSTNEE178 pKa = 3.6IITPSEE184 pKa = 4.34EE185 pKa = 3.85ISVSAILKK193 pKa = 9.72QLAALTAKK201 pKa = 10.12LDD203 pKa = 4.13KK204 pKa = 10.56IEE206 pKa = 4.28KK207 pKa = 9.91QNEE210 pKa = 3.76EE211 pKa = 4.52LIKK214 pKa = 10.81EE215 pKa = 4.19NNNLKK220 pKa = 9.3MEE222 pKa = 4.56RR223 pKa = 11.84EE224 pKa = 3.82QFLEE228 pKa = 4.08NVHH231 pKa = 7.1KK232 pKa = 10.42EE233 pKa = 4.11VEE235 pKa = 4.48LEE237 pKa = 4.17TKK239 pKa = 9.76PQEE242 pKa = 4.34KK243 pKa = 8.55KK244 pKa = 9.12TLNTKK249 pKa = 8.39KK250 pKa = 8.22TQQKK254 pKa = 10.42SLGVNLKK261 pKa = 8.34ITKK264 pKa = 9.11TKK266 pKa = 10.4IIGQEE271 pKa = 3.45EE272 pKa = 4.47SLQQKK277 pKa = 9.38FVDD280 pKa = 4.62KK281 pKa = 10.42PNKK284 pKa = 8.67PLKK287 pKa = 9.55PNKK290 pKa = 10.09NMLGDD295 pKa = 3.78KK296 pKa = 9.95LKK298 pKa = 11.12KK299 pKa = 10.28NIRR302 pKa = 11.84EE303 pKa = 4.21WYY305 pKa = 10.16NFDD308 pKa = 3.6SSKK311 pKa = 11.2LEE313 pKa = 3.81QHH315 pKa = 6.07KK316 pKa = 11.05KK317 pKa = 9.72EE318 pKa = 4.11VLSSVITNATFAEE331 pKa = 5.01KK332 pKa = 9.88IRR334 pKa = 11.84EE335 pKa = 4.16TGVPKK340 pKa = 10.57QKK342 pKa = 9.87IRR344 pKa = 11.84YY345 pKa = 5.68TSPVTLKK352 pKa = 10.16EE353 pKa = 4.21DD354 pKa = 3.39KK355 pKa = 10.86KK356 pKa = 10.51SIHH359 pKa = 6.36YY360 pKa = 9.85YY361 pKa = 9.75GYY363 pKa = 10.21KK364 pKa = 10.01PSGIPNKK371 pKa = 9.73VWWHH375 pKa = 4.88WVTTVTSVEE384 pKa = 4.13AYY386 pKa = 8.83EE387 pKa = 3.93KK388 pKa = 11.1AEE390 pKa = 3.76RR391 pKa = 11.84FLYY394 pKa = 10.39NQFKK398 pKa = 10.84RR399 pKa = 11.84EE400 pKa = 3.76MFIYY404 pKa = 7.93RR405 pKa = 11.84TKK407 pKa = 8.26WVKK410 pKa = 10.05YY411 pKa = 8.57SKK413 pKa = 10.56EE414 pKa = 3.83FNPYY418 pKa = 9.47LSEE421 pKa = 4.12PKK423 pKa = 9.38MVWMEE428 pKa = 3.95HH429 pKa = 5.66TSDD432 pKa = 3.53FDD434 pKa = 3.95IDD436 pKa = 3.86VSVAYY441 pKa = 10.26AFILKK446 pKa = 8.4WRR448 pKa = 11.84KK449 pKa = 8.7LVQTFKK455 pKa = 10.66PNKK458 pKa = 8.72PINSDD463 pKa = 3.1WYY465 pKa = 11.0KK466 pKa = 10.17STQQQQ471 pKa = 2.91

Molecular weight: 55.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3764

278

2081

941.0

106.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.526 ± 0.93

1.089 ± 0.475

5.367 ± 0.36

6.854 ± 0.551

3.905 ± 0.16

5.234 ± 0.856

1.674 ± 0.331

7.519 ± 0.395

7.625 ± 2.168

8.369 ± 0.598

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.129

5.712 ± 0.558

4.437 ± 1.059

4.888 ± 0.669

4.118 ± 0.332

6.509 ± 0.904

6.961 ± 0.535

7.067 ± 0.571

1.488 ± 0.317

3.294 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski