Arthrobacter phage BarretLemon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Marthavirus; Arthrobacter virus BarretLemon

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140G775|A0A140G775_9CAUD Uncharacterized protein OS=Arthrobacter phage BarretLemon OX=1796994 GN=48 PE=4 SV=1
MM1 pKa = 7.23TEE3 pKa = 3.67VRR5 pKa = 11.84HH6 pKa = 6.79SEE8 pKa = 4.03FTDD11 pKa = 3.58SEE13 pKa = 4.43GQTFIIEE20 pKa = 4.31SEE22 pKa = 4.32DD23 pKa = 3.58GVISIQTYY31 pKa = 9.69GGHH34 pKa = 6.6IDD36 pKa = 4.45EE37 pKa = 5.38LDD39 pKa = 3.5EE40 pKa = 4.72GNCAGCEE47 pKa = 3.94DD48 pKa = 4.97CIEE51 pKa = 4.29TVALFAVDD59 pKa = 3.4APKK62 pKa = 10.3IIVALQRR69 pKa = 11.84ALQHH73 pKa = 6.27AATTGEE79 pKa = 4.15YY80 pKa = 10.95GRR82 pKa = 11.84GEE84 pKa = 4.55TISGQCVDD92 pKa = 3.4IHH94 pKa = 5.72STHH97 pKa = 7.13PFNAVSQLQSYY108 pKa = 9.85DD109 pKa = 3.77EE110 pKa = 5.06PDD112 pKa = 3.89FSPDD116 pKa = 3.01SPHH119 pKa = 6.28YY120 pKa = 9.98GKK122 pKa = 9.34PHH124 pKa = 6.77EE125 pKa = 5.15DD126 pKa = 3.3CCKK129 pKa = 10.59

Molecular weight: 14.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140G764|A0A140G764_9CAUD Single-stranded DNA-binding protein OS=Arthrobacter phage BarretLemon OX=1796994 GN=37 PE=3 SV=1
MM1 pKa = 7.03TRR3 pKa = 11.84EE4 pKa = 3.86GLYY7 pKa = 10.5VRR9 pKa = 11.84GVEE12 pKa = 4.11HH13 pKa = 6.67SAAKK17 pKa = 10.49LRR19 pKa = 11.84DD20 pKa = 3.43ADD22 pKa = 3.87VVRR25 pKa = 11.84MRR27 pKa = 11.84EE28 pKa = 3.52IAAGEE33 pKa = 4.23RR34 pKa = 11.84ITLRR38 pKa = 11.84EE39 pKa = 3.6LATRR43 pKa = 11.84FGVTTTAVHH52 pKa = 7.07RR53 pKa = 11.84IITGAGWAHH62 pKa = 6.84LDD64 pKa = 3.63GPIRR68 pKa = 11.84EE69 pKa = 4.2PRR71 pKa = 11.84SYY73 pKa = 10.69TKK75 pKa = 10.59RR76 pKa = 11.84KK77 pKa = 9.34

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16141

44

877

204.3

22.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.554 ± 0.381

0.651 ± 0.1

6.288 ± 0.257

6.4 ± 0.36

2.974 ± 0.124

7.354 ± 0.412

1.821 ± 0.157

4.882 ± 0.241

4.721 ± 0.231

8.079 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.169

3.507 ± 0.232

4.832 ± 0.238

3.86 ± 0.207

6.177 ± 0.325

6.133 ± 0.3

6.809 ± 0.376

7.658 ± 0.255

1.623 ± 0.14

2.348 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski