Amycolatopsis alkalitolerans
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6455 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C4LZD7|A0A5C4LZD7_9PSEU PadR family transcriptional regulator OS=Amycolatopsis alkalitolerans OX=2547244 GN=FG385_22210 PE=4 SV=1
MM1 pKa = 7.16 SQPIQTLHH9 pKa = 6.18 EE10 pKa = 4.48 FVFNLISNADD20 pKa = 3.57 FGSAFLSDD28 pKa = 3.66 PASALSAAGLGDD40 pKa = 3.21 ITGADD45 pKa = 3.7 VQEE48 pKa = 4.26 VTPLVTDD55 pKa = 3.63 SMPAPVADD63 pKa = 3.55 AVEE66 pKa = 4.62 SGLASLPTDD75 pKa = 3.82 AFGTDD80 pKa = 4.18 DD81 pKa = 3.74 LHH83 pKa = 7.42 CALTHH88 pKa = 6.48 LEE90 pKa = 4.36 TVASVAQSLPDD101 pKa = 3.7 TALSTAGTLSSVTDD115 pKa = 3.43 AMGVSGTFQGSADD128 pKa = 3.61 GLATGMLASTPAGDD142 pKa = 3.57 VTGALAATTGSLGAGMEE159 pKa = 4.56 SPLGTYY165 pKa = 10.44 GITTDD170 pKa = 4.05 SLPLSVPSFGSVSDD184 pKa = 3.97 LGGTLDD190 pKa = 3.99 SAALTGGTPVTEE202 pKa = 4.29 TATSYY207 pKa = 8.58 VTSGTEE213 pKa = 3.65 MATSGIANGSAMLGDD228 pKa = 5.21 HH229 pKa = 7.08 ISTAGTGLGGAVTAGGADD247 pKa = 3.87 LANHH251 pKa = 5.14 VTSAVSMAGDD261 pKa = 3.89 LTSNLPTVSAPVHH274 pKa = 6.33 LPADD278 pKa = 3.95 LPVHH282 pKa = 6.46 VVAQPPALPAADD294 pKa = 3.38 AAVPQVTHH302 pKa = 5.45 TVEE305 pKa = 4.3 STVSHH310 pKa = 6.73 ASVLPDD316 pKa = 3.6 SLSHH320 pKa = 6.94 GSLAGMAHH328 pKa = 6.02 SAVPGVDD335 pKa = 3.41 SLHH338 pKa = 6.5 TDD340 pKa = 4.02 LLHH343 pKa = 7.34 GEE345 pKa = 4.57 LPLGHH350 pKa = 7.14
Molecular weight: 33.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.643
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.266
Thurlkill 3.706
EMBOSS 3.834
Sillero 3.999
Patrickios 0.871
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|A0A5C4M5P3|A0A5C4M5P3_9PSEU BldC family transcriptional regulator OS=Amycolatopsis alkalitolerans OX=2547244 GN=FG385_04725 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.72 HH17 pKa = 5.63 RR18 pKa = 11.84 KK19 pKa = 7.68 LLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 MQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 9.69 QGKK33 pKa = 8.53
Molecular weight: 4.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6455
0
6455
2018279
27
2075
312.7
33.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.215 ± 0.045
0.793 ± 0.01
5.663 ± 0.024
5.775 ± 0.028
2.97 ± 0.019
9.339 ± 0.03
2.237 ± 0.014
3.696 ± 0.019
2.13 ± 0.02
10.581 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.778 ± 0.012
1.912 ± 0.018
5.798 ± 0.025
2.799 ± 0.016
8.004 ± 0.035
5.083 ± 0.021
5.814 ± 0.024
8.873 ± 0.025
1.478 ± 0.013
2.064 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here