Amino acid dipepetide frequency for Synechococcus phage S-CRM01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.985AlaAla: 3.985 ± 0.507
0.636AlaCys: 0.636 ± 0.112
2.975AlaAsp: 2.975 ± 0.252
3.967AlaGlu: 3.967 ± 0.25
2.301AlaPhe: 2.301 ± 0.203
4.434AlaGly: 4.434 ± 0.386
0.823AlaHis: 0.823 ± 0.118
3.461AlaIle: 3.461 ± 0.249
3.574AlaLys: 3.574 ± 0.276
4.734AlaLeu: 4.734 ± 0.337
1.347AlaMet: 1.347 ± 0.15
3.518AlaAsn: 3.518 ± 0.28
2.451AlaPro: 2.451 ± 0.272
2.507AlaGln: 2.507 ± 0.207
2.619AlaArg: 2.619 ± 0.232
3.592AlaSer: 3.592 ± 0.267
3.667AlaThr: 3.667 ± 0.34
3.106AlaVal: 3.106 ± 0.258
0.861AlaTrp: 0.861 ± 0.143
2.152AlaTyr: 2.152 ± 0.233
0.0AlaXaa: 0.0 ± 0.0
Cys
0.43CysAla: 0.43 ± 0.089
0.112CysCys: 0.112 ± 0.046
0.842CysAsp: 0.842 ± 0.112
0.505CysGlu: 0.505 ± 0.093
0.299CysPhe: 0.299 ± 0.081
1.066CysGly: 1.066 ± 0.146
0.262CysHis: 0.262 ± 0.076
0.599CysIle: 0.599 ± 0.11
0.58CysLys: 0.58 ± 0.121
0.73CysLeu: 0.73 ± 0.113
0.262CysMet: 0.262 ± 0.073
0.805CysAsn: 0.805 ± 0.125
0.355CysPro: 0.355 ± 0.07
0.243CysGln: 0.243 ± 0.065
0.561CysArg: 0.561 ± 0.127
0.805CysSer: 0.805 ± 0.124
0.543CysThr: 0.543 ± 0.085
0.748CysVal: 0.748 ± 0.119
0.281CysTrp: 0.281 ± 0.072
0.468CysTyr: 0.468 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
3.274AspAla: 3.274 ± 0.27
0.674AspCys: 0.674 ± 0.133
3.91AspAsp: 3.91 ± 0.303
5.052AspGlu: 5.052 ± 0.34
2.713AspPhe: 2.713 ± 0.193
5.033AspGly: 5.033 ± 0.413
1.272AspHis: 1.272 ± 0.166
4.696AspIle: 4.696 ± 0.211
3.967AspLys: 3.967 ± 0.29
5.332AspLeu: 5.332 ± 0.332
1.534AspMet: 1.534 ± 0.177
2.844AspAsn: 2.844 ± 0.223
3.461AspPro: 3.461 ± 0.272
1.852AspGln: 1.852 ± 0.164
2.47AspArg: 2.47 ± 0.209
4.172AspSer: 4.172 ± 0.3
3.405AspThr: 3.405 ± 0.275
4.378AspVal: 4.378 ± 0.252
1.048AspTrp: 1.048 ± 0.139
3.368AspTyr: 3.368 ± 0.257
0.0AspXaa: 0.0 ± 0.0
Glu
3.256GluAla: 3.256 ± 0.276
0.786GluCys: 0.786 ± 0.179
5.145GluAsp: 5.145 ± 0.314
5.594GluGlu: 5.594 ± 0.455
3.199GluPhe: 3.199 ± 0.208
3.761GluGly: 3.761 ± 0.26
1.066GluHis: 1.066 ± 0.171
4.752GluIle: 4.752 ± 0.302
4.734GluLys: 4.734 ± 0.409
6.698GluLeu: 6.698 ± 0.371
1.478GluMet: 1.478 ± 0.193
3.424GluAsn: 3.424 ± 0.241
2.189GluPro: 2.189 ± 0.2
2.619GluGln: 2.619 ± 0.272
2.956GluArg: 2.956 ± 0.22
4.116GluSer: 4.116 ± 0.297
3.723GluThr: 3.723 ± 0.267
5.183GluVal: 5.183 ± 0.398
1.01GluTrp: 1.01 ± 0.146
3.012GluTyr: 3.012 ± 0.257
0.0GluXaa: 0.0 ± 0.0
Phe
2.432PheAla: 2.432 ± 0.226
0.449PheCys: 0.449 ± 0.102
3.555PheAsp: 3.555 ± 0.292
2.432PheGlu: 2.432 ± 0.23
1.385PhePhe: 1.385 ± 0.163
2.619PheGly: 2.619 ± 0.282
0.636PheHis: 0.636 ± 0.131
2.825PheIle: 2.825 ± 0.216
2.582PheLys: 2.582 ± 0.221
2.975PheLeu: 2.975 ± 0.246
1.066PheMet: 1.066 ± 0.119
2.545PheAsn: 2.545 ± 0.22
2.002PhePro: 2.002 ± 0.231
2.189PheGln: 2.189 ± 0.477
1.777PheArg: 1.777 ± 0.178
2.881PheSer: 2.881 ± 0.199
3.087PheThr: 3.087 ± 0.245
2.825PheVal: 2.825 ± 0.227
0.543PheTrp: 0.543 ± 0.102
2.189PheTyr: 2.189 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
4.098GlyAla: 4.098 ± 0.405
0.767GlyCys: 0.767 ± 0.125
4.098GlyAsp: 4.098 ± 0.291
4.116GlyGlu: 4.116 ± 0.256
3.742GlyPhe: 3.742 ± 0.48
5.314GlyGly: 5.314 ± 0.489
1.272GlyHis: 1.272 ± 0.159
4.565GlyIle: 4.565 ± 0.268
3.667GlyLys: 3.667 ± 0.286
4.846GlyLeu: 4.846 ± 0.35
1.497GlyMet: 1.497 ± 0.198
3.798GlyAsn: 3.798 ± 0.382
3.274GlyPro: 3.274 ± 0.519
2.601GlyGln: 2.601 ± 0.394
3.087GlyArg: 3.087 ± 0.258
5.276GlySer: 5.276 ± 0.511
5.033GlyThr: 5.033 ± 0.507
4.584GlyVal: 4.584 ± 0.314
1.31GlyTrp: 1.31 ± 0.155
2.732GlyTyr: 2.732 ± 0.202
0.0GlyXaa: 0.0 ± 0.0
His
0.711HisAla: 0.711 ± 0.091
0.318HisCys: 0.318 ± 0.085
1.31HisAsp: 1.31 ± 0.192
0.917HisGlu: 0.917 ± 0.135
0.767HisPhe: 0.767 ± 0.12
1.085HisGly: 1.085 ± 0.169
0.299HisHis: 0.299 ± 0.075
1.01HisIle: 1.01 ± 0.153
0.992HisLys: 0.992 ± 0.152
1.347HisLeu: 1.347 ± 0.147
0.505HisMet: 0.505 ± 0.087
0.767HisAsn: 0.767 ± 0.129
1.066HisPro: 1.066 ± 0.15
0.748HisGln: 0.748 ± 0.124
0.599HisArg: 0.599 ± 0.107
0.992HisSer: 0.992 ± 0.155
0.823HisThr: 0.823 ± 0.127
0.936HisVal: 0.936 ± 0.127
0.468HisTrp: 0.468 ± 0.098
1.01HisTyr: 1.01 ± 0.168
0.0HisXaa: 0.0 ± 0.0
Ile
3.817IleAla: 3.817 ± 0.236
0.917IleCys: 0.917 ± 0.124
4.79IleAsp: 4.79 ± 0.287
4.958IleGlu: 4.958 ± 0.316
2.601IlePhe: 2.601 ± 0.244
3.929IleGly: 3.929 ± 0.264
1.141IleHis: 1.141 ± 0.148
4.191IleIle: 4.191 ± 0.332
3.779IleLys: 3.779 ± 0.325
5.258IleLeu: 5.258 ± 0.347
1.123IleMet: 1.123 ± 0.153
3.611IleAsn: 3.611 ± 0.25
3.143IlePro: 3.143 ± 0.329
2.9IleGln: 2.9 ± 0.207
3.162IleArg: 3.162 ± 0.291
4.771IleSer: 4.771 ± 0.323
4.509IleThr: 4.509 ± 0.352
3.967IleVal: 3.967 ± 0.292
0.73IleTrp: 0.73 ± 0.121
2.638IleTyr: 2.638 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
3.012LysAla: 3.012 ± 0.311
0.524LysCys: 0.524 ± 0.106
3.536LysAsp: 3.536 ± 0.331
5.557LysGlu: 5.557 ± 0.506
2.844LysPhe: 2.844 ± 0.243
3.143LysGly: 3.143 ± 0.258
1.291LysHis: 1.291 ± 0.168
3.667LysIle: 3.667 ± 0.336
5.145LysLys: 5.145 ± 0.462
6.118LysLeu: 6.118 ± 0.417
1.777LysMet: 1.777 ± 0.223
3.536LysAsn: 3.536 ± 0.27
2.507LysPro: 2.507 ± 0.262
2.507LysGln: 2.507 ± 0.214
2.488LysArg: 2.488 ± 0.28
3.518LysSer: 3.518 ± 0.261
3.461LysThr: 3.461 ± 0.278
3.817LysVal: 3.817 ± 0.279
0.73LysTrp: 0.73 ± 0.114
2.676LysTyr: 2.676 ± 0.22
0.0LysXaa: 0.0 ± 0.0
Leu
4.752LeuAla: 4.752 ± 0.331
0.767LeuCys: 0.767 ± 0.122
5.969LeuAsp: 5.969 ± 0.369
5.875LeuGlu: 5.875 ± 0.39
3.143LeuPhe: 3.143 ± 0.269
4.584LeuGly: 4.584 ± 0.302
1.254LeuHis: 1.254 ± 0.165
4.846LeuIle: 4.846 ± 0.357
5.482LeuLys: 5.482 ± 0.423
5.931LeuLeu: 5.931 ± 0.358
1.609LeuMet: 1.609 ± 0.199
5.781LeuAsn: 5.781 ± 0.293
4.004LeuPro: 4.004 ± 0.242
2.807LeuGln: 2.807 ± 0.218
3.555LeuArg: 3.555 ± 0.235
5.052LeuSer: 5.052 ± 0.308
5.164LeuThr: 5.164 ± 0.274
4.472LeuVal: 4.472 ± 0.236
0.917LeuTrp: 0.917 ± 0.15
3.031LeuTyr: 3.031 ± 0.208
0.0LeuXaa: 0.0 ± 0.0
Met
1.497MetAla: 1.497 ± 0.207
0.262MetCys: 0.262 ± 0.056
1.048MetAsp: 1.048 ± 0.156
1.516MetGlu: 1.516 ± 0.178
0.954MetPhe: 0.954 ± 0.134
1.197MetGly: 1.197 ± 0.164
0.486MetHis: 0.486 ± 0.089
1.385MetIle: 1.385 ± 0.186
1.946MetLys: 1.946 ± 0.226
1.572MetLeu: 1.572 ± 0.205
0.617MetMet: 0.617 ± 0.098
1.609MetAsn: 1.609 ± 0.169
0.674MetPro: 0.674 ± 0.118
0.786MetGln: 0.786 ± 0.114
1.179MetArg: 1.179 ± 0.156
1.74MetSer: 1.74 ± 0.194
2.133MetThr: 2.133 ± 0.213
1.385MetVal: 1.385 ± 0.182
0.206MetTrp: 0.206 ± 0.068
0.617MetTyr: 0.617 ± 0.121
0.0MetXaa: 0.0 ± 0.0
Asn
2.9AsnAla: 2.9 ± 0.243
0.524AsnCys: 0.524 ± 0.093
3.443AsnAsp: 3.443 ± 0.221
3.087AsnGlu: 3.087 ± 0.219
2.676AsnPhe: 2.676 ± 0.222
5.052AsnGly: 5.052 ± 0.445
0.842AsnHis: 0.842 ± 0.127
4.341AsnIle: 4.341 ± 0.277
3.087AsnLys: 3.087 ± 0.274
4.285AsnLeu: 4.285 ± 0.296
1.085AsnMet: 1.085 ± 0.155
3.48AsnAsn: 3.48 ± 0.299
3.499AsnPro: 3.499 ± 0.26
2.376AsnGln: 2.376 ± 0.196
3.087AsnArg: 3.087 ± 0.278
4.079AsnSer: 4.079 ± 0.351
3.48AsnThr: 3.48 ± 0.289
3.499AsnVal: 3.499 ± 0.292
0.898AsnTrp: 0.898 ± 0.129
3.106AsnTyr: 3.106 ± 0.239
0.019AsnXaa: 0.019 ± 0.018
Pro
2.863ProAla: 2.863 ± 0.323
0.225ProCys: 0.225 ± 0.074
2.788ProAsp: 2.788 ± 0.291
3.256ProGlu: 3.256 ± 0.287
2.17ProPhe: 2.17 ± 0.19
3.368ProGly: 3.368 ± 0.3
0.599ProHis: 0.599 ± 0.102
2.488ProIle: 2.488 ± 0.221
2.788ProLys: 2.788 ± 0.282
3.125ProLeu: 3.125 ± 0.209
0.954ProMet: 0.954 ± 0.122
2.713ProAsn: 2.713 ± 0.265
2.245ProPro: 2.245 ± 0.28
2.451ProGln: 2.451 ± 0.511
1.684ProArg: 1.684 ± 0.185
3.087ProSer: 3.087 ± 0.24
4.023ProThr: 4.023 ± 0.293
2.788ProVal: 2.788 ± 0.286
0.674ProTrp: 0.674 ± 0.114
1.815ProTyr: 1.815 ± 0.17
0.0ProXaa: 0.0 ± 0.0
Gln
2.152GlnAla: 2.152 ± 0.195
0.355GlnCys: 0.355 ± 0.077
1.983GlnAsp: 1.983 ± 0.168
2.676GlnGlu: 2.676 ± 0.214
1.665GlnPhe: 1.665 ± 0.158
5.201GlnGly: 5.201 ± 1.475
0.486GlnHis: 0.486 ± 0.089
2.357GlnIle: 2.357 ± 0.197
2.021GlnLys: 2.021 ± 0.214
3.499GlnLeu: 3.499 ± 0.274
0.898GlnMet: 0.898 ± 0.143
2.283GlnAsn: 2.283 ± 0.219
1.347GlnPro: 1.347 ± 0.155
1.422GlnGln: 1.422 ± 0.171
1.646GlnArg: 1.646 ± 0.16
2.601GlnSer: 2.601 ± 0.218
2.395GlnThr: 2.395 ± 0.21
2.152GlnVal: 2.152 ± 0.201
0.58GlnTrp: 0.58 ± 0.125
1.834GlnTyr: 1.834 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
2.937ArgAla: 2.937 ± 0.206
0.468ArgCys: 0.468 ± 0.087
2.975ArgAsp: 2.975 ± 0.197
2.769ArgGlu: 2.769 ± 0.222
1.815ArgPhe: 1.815 ± 0.169
2.582ArgGly: 2.582 ± 0.208
0.486ArgHis: 0.486 ± 0.097
3.387ArgIle: 3.387 ± 0.276
2.638ArgLys: 2.638 ± 0.234
3.648ArgLeu: 3.648 ± 0.262
1.029ArgMet: 1.029 ± 0.131
2.788ArgAsn: 2.788 ± 0.202
1.497ArgPro: 1.497 ± 0.183
1.815ArgGln: 1.815 ± 0.219
2.17ArgArg: 2.17 ± 0.218
2.788ArgSer: 2.788 ± 0.244
2.414ArgThr: 2.414 ± 0.218
2.732ArgVal: 2.732 ± 0.208
0.505ArgTrp: 0.505 ± 0.092
2.189ArgTyr: 2.189 ± 0.181
0.0ArgXaa: 0.0 ± 0.0
Ser
3.91SerAla: 3.91 ± 0.373
0.655SerCys: 0.655 ± 0.109
4.079SerAsp: 4.079 ± 0.271
4.004SerGlu: 4.004 ± 0.351
2.994SerPhe: 2.994 ± 0.231
5.108SerGly: 5.108 ± 0.399
1.029SerHis: 1.029 ± 0.149
4.659SerIle: 4.659 ± 0.318
3.667SerLys: 3.667 ± 0.258
5.276SerLeu: 5.276 ± 0.317
1.815SerMet: 1.815 ± 0.181
4.397SerAsn: 4.397 ± 0.343
3.012SerPro: 3.012 ± 0.257
2.638SerGln: 2.638 ± 0.214
2.619SerArg: 2.619 ± 0.241
5.445SerSer: 5.445 ± 0.385
4.434SerThr: 4.434 ± 0.301
4.509SerVal: 4.509 ± 0.324
1.029SerTrp: 1.029 ± 0.159
2.357SerTyr: 2.357 ± 0.212
0.037SerXaa: 0.037 ± 0.029
Thr
3.779ThrAla: 3.779 ± 0.286
0.58ThrCys: 0.58 ± 0.125
3.779ThrAsp: 3.779 ± 0.261
4.116ThrGlu: 4.116 ± 0.279
2.732ThrPhe: 2.732 ± 0.279
4.659ThrGly: 4.659 ± 0.396
1.291ThrHis: 1.291 ± 0.152
4.378ThrIle: 4.378 ± 0.303
4.135ThrLys: 4.135 ± 0.28
4.752ThrLeu: 4.752 ± 0.356
1.235ThrMet: 1.235 ± 0.129
3.929ThrAsn: 3.929 ± 0.327
3.742ThrPro: 3.742 ± 0.259
2.825ThrGln: 2.825 ± 0.398
2.264ThrArg: 2.264 ± 0.222
4.472ThrSer: 4.472 ± 0.428
4.547ThrThr: 4.547 ± 0.43
4.079ThrVal: 4.079 ± 0.328
0.879ThrTrp: 0.879 ± 0.166
2.301ThrTyr: 2.301 ± 0.172
0.0ThrXaa: 0.0 ± 0.0
Val
3.985ValAla: 3.985 ± 0.306
0.692ValCys: 0.692 ± 0.106
4.154ValAsp: 4.154 ± 0.28
4.621ValGlu: 4.621 ± 0.324
2.788ValPhe: 2.788 ± 0.229
4.266ValGly: 4.266 ± 0.285
0.73ValHis: 0.73 ± 0.127
4.228ValIle: 4.228 ± 0.318
3.761ValLys: 3.761 ± 0.3
4.397ValLeu: 4.397 ± 0.291
1.534ValMet: 1.534 ± 0.195
3.817ValAsn: 3.817 ± 0.27
3.256ValPro: 3.256 ± 0.275
2.021ValGln: 2.021 ± 0.192
2.844ValArg: 2.844 ± 0.269
4.416ValSer: 4.416 ± 0.363
4.191ValThr: 4.191 ± 0.454
3.948ValVal: 3.948 ± 0.313
0.917ValTrp: 0.917 ± 0.118
2.619ValTyr: 2.619 ± 0.208
0.0ValXaa: 0.0 ± 0.0
Trp
1.066TrpAla: 1.066 ± 0.168
0.15TrpCys: 0.15 ± 0.065
1.104TrpAsp: 1.104 ± 0.174
0.842TrpGlu: 0.842 ± 0.126
0.543TrpPhe: 0.543 ± 0.114
0.805TrpGly: 0.805 ± 0.139
0.299TrpHis: 0.299 ± 0.075
0.954TrpIle: 0.954 ± 0.14
0.842TrpLys: 0.842 ± 0.151
0.767TrpLeu: 0.767 ± 0.127
0.337TrpMet: 0.337 ± 0.09
1.029TrpAsn: 1.029 ± 0.141
0.636TrpPro: 0.636 ± 0.124
0.543TrpGln: 0.543 ± 0.097
0.786TrpArg: 0.786 ± 0.153
1.029TrpSer: 1.029 ± 0.148
0.842TrpThr: 0.842 ± 0.126
1.141TrpVal: 1.141 ± 0.17
0.468TrpTrp: 0.468 ± 0.103
0.468TrpTyr: 0.468 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.852TyrAla: 1.852 ± 0.194
0.636TyrCys: 0.636 ± 0.122
2.975TyrAsp: 2.975 ± 0.273
2.844TyrGlu: 2.844 ± 0.234
1.646TyrPhe: 1.646 ± 0.15
2.47TyrGly: 2.47 ± 0.26
1.104TyrHis: 1.104 ± 0.154
3.143TyrIle: 3.143 ± 0.249
2.47TyrLys: 2.47 ± 0.22
3.667TyrLeu: 3.667 ± 0.25
1.085TyrMet: 1.085 ± 0.17
2.189TyrAsn: 2.189 ± 0.211
1.74TyrPro: 1.74 ± 0.192
1.703TyrGln: 1.703 ± 0.149
2.077TyrArg: 2.077 ± 0.169
2.844TyrSer: 2.844 ± 0.241
2.601TyrThr: 2.601 ± 0.221
2.937TyrVal: 2.937 ± 0.214
0.599TyrTrp: 0.599 ± 0.094
1.946TyrTyr: 1.946 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.019XaaPhe: 0.019 ± 0.021
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.037XaaLeu: 0.037 ± 0.023
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 297 proteins (53448 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski