Hedyotis uncinella yellow mosaic virus
Average proteome isoelectric point is 8.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5S0Z4|S5S0Z4_9GEMI Protein V2 OS=Hedyotis uncinella yellow mosaic virus OX=1428190 PE=3 SV=1
MM1 pKa = 7.88 PPPKK5 pKa = 10.14 KK6 pKa = 10.34 FQINAKK12 pKa = 9.94 NYY14 pKa = 7.96 FLTYY18 pKa = 9.06 PKK20 pKa = 10.53 CSLTKK25 pKa = 10.62 EE26 pKa = 3.61 EE27 pKa = 5.48 ALAQFQNLDD36 pKa = 2.94 IPINKK41 pKa = 9.45 KK42 pKa = 9.63 YY43 pKa = 10.79 IKK45 pKa = 9.69 ICRR48 pKa = 11.84 EE49 pKa = 3.74 LHH51 pKa = 6.25 EE52 pKa = 5.87 DD53 pKa = 4.22 GSPHH57 pKa = 6.5 LHH59 pKa = 6.04 VLVQFEE65 pKa = 4.27 GKK67 pKa = 9.66 YY68 pKa = 9.5 RR69 pKa = 11.84 CTNNRR74 pKa = 11.84 FFDD77 pKa = 3.9 LVSPSRR83 pKa = 11.84 SAHH86 pKa = 4.9 FHH88 pKa = 6.45 PNIQGAKK95 pKa = 9.25 SSSDD99 pKa = 3.11 VKK101 pKa = 11.24 SYY103 pKa = 10.79 IDD105 pKa = 3.7 KK106 pKa = 11.33 DD107 pKa = 3.49 GDD109 pKa = 3.67 TLEE112 pKa = 4.14 WGEE115 pKa = 3.94 FQIDD119 pKa = 3.13 ARR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQSANDD132 pKa = 4.12 AYY134 pKa = 10.84 AKK136 pKa = 10.61 ALNTGSKK143 pKa = 10.29 SEE145 pKa = 3.93 ALNVIRR151 pKa = 11.84 EE152 pKa = 4.21 LAPKK156 pKa = 10.52 DD157 pKa = 3.64 FVLQFHH163 pKa = 6.68 NLNSNLDD170 pKa = 4.08 RR171 pKa = 11.84 IFTPPAEE178 pKa = 4.47 VYY180 pKa = 10.38 VSPFSSSSFDD190 pKa = 3.41 QVPEE194 pKa = 3.96 EE195 pKa = 4.18 LEE197 pKa = 4.15 CWVSEE202 pKa = 4.13 NVMGAAARR210 pKa = 11.84 PWRR213 pKa = 11.84 PNSIVIEE220 pKa = 4.34 GDD222 pKa = 3.08 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.02 VYY251 pKa = 10.89 SNEE254 pKa = 3.02 AWYY257 pKa = 10.82 NVIDD261 pKa = 5.29 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.46 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.26 YY287 pKa = 10.63 GKK289 pKa = 9.49 PIQIKK294 pKa = 10.42 GGIPTIFLCNPGPTSSYY311 pKa = 11.03 KK312 pKa = 10.55 EE313 pKa = 3.94 YY314 pKa = 10.94 LDD316 pKa = 3.87 EE317 pKa = 4.82 EE318 pKa = 4.67 KK319 pKa = 11.16 NSALKK324 pKa = 10.45 AWAIKK329 pKa = 9.72 NAEE332 pKa = 4.62 FITLTEE338 pKa = 4.09 PLYY341 pKa = 11.19 SGTNQSPAQNSQEE354 pKa = 3.98 KK355 pKa = 9.31 TNPEE359 pKa = 3.98 TEE361 pKa = 4.16 GG362 pKa = 3.3
Molecular weight: 40.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.884
IPC2_protein 5.881
IPC_protein 5.944
Toseland 6.313
ProMoST 6.313
Dawson 6.237
Bjellqvist 6.224
Wikipedia 6.249
Rodwell 6.224
Grimsley 6.465
Solomon 6.237
Lehninger 6.224
Nozaki 6.504
DTASelect 6.693
Thurlkill 6.707
EMBOSS 6.678
Sillero 6.605
Patrickios 4.101
IPC_peptide 6.249
IPC2_peptide 6.532
IPC2.peptide.svr19 6.561
Protein with the highest isoelectric point:
>tr|S5REP9|S5REP9_9GEMI AC5 protein OS=Hedyotis uncinella yellow mosaic virus OX=1428190 PE=4 SV=1
MM1 pKa = 7.63 SKK3 pKa = 10.39 RR4 pKa = 11.84 PADD7 pKa = 3.69 MVFSTPVSRR16 pKa = 11.84 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.26 TPGVTRR30 pKa = 11.84 VAAPTVRR37 pKa = 11.84 ATNRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 MWINRR48 pKa = 11.84 PMYY51 pKa = 9.4 RR52 pKa = 11.84 KK53 pKa = 8.79 PRR55 pKa = 11.84 IYY57 pKa = 10.75 RR58 pKa = 11.84 MYY60 pKa = 10.25 RR61 pKa = 11.84 SPDD64 pKa = 3.16 VPYY67 pKa = 10.77 GCEE70 pKa = 4.42 GPCKK74 pKa = 10.14 VQSFEE79 pKa = 3.84 QRR81 pKa = 11.84 HH82 pKa = 6.27 DD83 pKa = 3.18 IAHH86 pKa = 6.1 TGKK89 pKa = 10.34 VICVSDD95 pKa = 3.47 VTRR98 pKa = 11.84 GNGLTHH104 pKa = 6.89 RR105 pKa = 11.84 VGKK108 pKa = 9.66 RR109 pKa = 11.84 FCVKK113 pKa = 9.94 SVYY116 pKa = 10.62 VLGKK120 pKa = 9.71 VWMDD124 pKa = 3.25 EE125 pKa = 4.06 NIKK128 pKa = 9.31 TKK130 pKa = 10.61 NHH132 pKa = 6.03 TNTVMFFVVRR142 pKa = 11.84 DD143 pKa = 3.74 RR144 pKa = 11.84 RR145 pKa = 11.84 PFGTPMDD152 pKa = 4.58 FGQVFNMYY160 pKa = 10.66 DD161 pKa = 3.86 NEE163 pKa = 4.34 PSTATVKK170 pKa = 10.66 NDD172 pKa = 2.91 LRR174 pKa = 11.84 GRR176 pKa = 11.84 YY177 pKa = 8.35 QVLRR181 pKa = 11.84 RR182 pKa = 11.84 FTAMATGGQYY192 pKa = 10.93 ASKK195 pKa = 10.11 EE196 pKa = 3.81 QALVRR201 pKa = 11.84 KK202 pKa = 9.46 FMKK205 pKa = 10.51 LNNHH209 pKa = 4.96 VCYY212 pKa = 10.35 NHH214 pKa = 6.24 QEE216 pKa = 3.69 AAKK219 pKa = 10.4 YY220 pKa = 9.25 EE221 pKa = 4.13 NHH223 pKa = 6.46 TEE225 pKa = 3.99 NALLLYY231 pKa = 7.29 MACTHH236 pKa = 7.07 ASNPVYY242 pKa = 9.86 ATLKK246 pKa = 9.47 IRR248 pKa = 11.84 IYY250 pKa = 10.67 FYY252 pKa = 11.26 DD253 pKa = 3.51 SVSNN257 pKa = 3.9
Molecular weight: 29.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.663
IPC_protein 10.189
Toseland 10.204
ProMoST 9.97
Dawson 10.423
Bjellqvist 10.145
Wikipedia 10.628
Rodwell 10.687
Grimsley 10.511
Solomon 10.467
Lehninger 10.423
Nozaki 10.233
DTASelect 10.131
Thurlkill 10.277
EMBOSS 10.613
Sillero 10.35
Patrickios 10.204
IPC_peptide 10.452
IPC2_peptide 9.18
IPC2.peptide.svr19 8.523
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
1486
96
362
165.1
18.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.374 ± 0.815
2.355 ± 0.455
4.576 ± 0.35
4.307 ± 0.71
4.105 ± 0.616
4.643 ± 0.356
3.836 ± 0.501
7.201 ± 1.23
4.98 ± 0.814
8.546 ± 1.418
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.826 ± 0.647
5.518 ± 0.616
5.989 ± 0.692
4.24 ± 0.423
7.402 ± 1.264
8.412 ± 1.294
6.46 ± 0.768
5.855 ± 0.98
1.346 ± 0.355
3.028 ± 0.649
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here