Cedratvirus Zaza IHUMI
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 636 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R8FCS7|A0A2R8FCS7_9VIRU SKP1/BTB/POZ domain-containing protein OS=Cedratvirus Zaza IHUMI OX=2126979 GN=ZAZAV_19 PE=4 SV=1
MM1 pKa = 7.61 SSSTYY6 pKa = 9.47 LTFNIVRR13 pKa = 11.84 NGNLLQVHH21 pKa = 5.99 TEE23 pKa = 3.64 ATLPQGKK30 pKa = 9.03 LVIDD34 pKa = 4.08 AEE36 pKa = 4.65 GSSFVSLRR44 pKa = 11.84 YY45 pKa = 9.82 NINSALIYY53 pKa = 10.81 ARR55 pKa = 11.84 DD56 pKa = 3.43 EE57 pKa = 4.13 LGILFTRR64 pKa = 11.84 PYY66 pKa = 9.93 KK67 pKa = 10.37 IVWRR71 pKa = 11.84 EE72 pKa = 3.35 NGQYY76 pKa = 7.99 QEE78 pKa = 4.02 RR79 pKa = 11.84 VYY81 pKa = 10.74 TVDD84 pKa = 5.17 DD85 pKa = 3.95 NLPYY89 pKa = 10.18 HH90 pKa = 6.79 ASFVGDD96 pKa = 3.33 YY97 pKa = 10.74 SVEE100 pKa = 4.07 LVNLEE105 pKa = 4.04 VLEE108 pKa = 4.26 EE109 pKa = 4.25 EE110 pKa = 5.13 EE111 pKa = 5.07 DD112 pKa = 3.99 EE113 pKa = 5.08 FWNEE117 pKa = 3.69 DD118 pKa = 3.43 DD119 pKa = 4.86 RR120 pKa = 11.84 EE121 pKa = 3.94 EE122 pKa = 5.23 DD123 pKa = 3.21 EE124 pKa = 6.73 FYY126 pKa = 11.28 DD127 pKa = 4.21 EE128 pKa = 5.2 LDD130 pKa = 4.14 DD131 pKa = 4.98 YY132 pKa = 11.81 EE133 pKa = 4.49 EE134 pKa = 4.17 EE135 pKa = 5.0 PYY137 pKa = 10.75 LQEE140 pKa = 4.1 EE141 pKa = 4.45 EE142 pKa = 5.0 EE143 pKa = 4.57 YY144 pKa = 11.36
Molecular weight: 17.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.91
IPC_protein 3.859
Toseland 3.681
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.681
Rodwell 3.681
Grimsley 3.592
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.05
Thurlkill 3.694
EMBOSS 3.694
Sillero 3.961
Patrickios 0.769
IPC_peptide 3.795
IPC2_peptide 3.948
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A2R8FFX0|A0A2R8FFX0_9VIRU Uncharacterized protein OS=Cedratvirus Zaza IHUMI OX=2126979 GN=ZAZAV_583 PE=4 SV=1
MM1 pKa = 7.43 GRR3 pKa = 11.84 RR4 pKa = 11.84 SHH6 pKa = 6.98 RR7 pKa = 11.84 KK8 pKa = 7.48 IKK10 pKa = 9.09 QRR12 pKa = 11.84 KK13 pKa = 8.6 RR14 pKa = 11.84 MVLPHH19 pKa = 6.29 PRR21 pKa = 11.84 SNKK24 pKa = 9.63 GDD26 pKa = 3.54 TGSGKK31 pKa = 10.18 VKK33 pKa = 10.02 EE34 pKa = 4.26 SRR36 pKa = 11.84 IEE38 pKa = 3.74 VSPNCKK44 pKa = 9.16 PLWWSVHH51 pKa = 4.8 QEE53 pKa = 3.86 ALKK56 pKa = 8.86 QTSPSARR63 pKa = 11.84 FEE65 pKa = 3.99 MYY67 pKa = 10.34 DD68 pKa = 4.04 IIDD71 pKa = 3.52 SYY73 pKa = 11.43 PRR75 pKa = 11.84 VRR77 pKa = 11.84 GQSRR81 pKa = 11.84 PFDD84 pKa = 3.58 NPRR87 pKa = 11.84 FLHH90 pKa = 6.77 EE91 pKa = 6.44 DD92 pKa = 4.15 FFQYY96 pKa = 9.69 TWDD99 pKa = 3.38 LYY101 pKa = 11.23 KK102 pKa = 10.37 RR103 pKa = 11.84 VHH105 pKa = 5.86 QRR107 pKa = 11.84 INHH110 pKa = 5.25 II111 pKa = 3.9
Molecular weight: 13.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.706
IPC_protein 10.379
Toseland 10.687
ProMoST 10.321
Dawson 10.774
Bjellqvist 10.452
Wikipedia 10.965
Rodwell 11.082
Grimsley 10.833
Solomon 10.877
Lehninger 10.847
Nozaki 10.657
DTASelect 10.452
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.818
IPC_peptide 10.877
IPC2_peptide 9.268
IPC2.peptide.svr19 8.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
636
0
636
157024
50
1579
246.9
28.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.482 ± 0.105
2.386 ± 0.081
5.153 ± 0.069
7.603 ± 0.098
4.565 ± 0.074
5.879 ± 0.176
1.961 ± 0.051
5.558 ± 0.083
6.199 ± 0.115
10.738 ± 0.138
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.944 ± 0.046
4.435 ± 0.075
4.176 ± 0.082
3.527 ± 0.053
5.376 ± 0.076
7.414 ± 0.107
5.012 ± 0.135
6.45 ± 0.077
1.486 ± 0.061
4.654 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here