Diporeia-associated CRESS-DNA virus LM122

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5SRU9|A0A1W5SRU9_9VIRU Putative replication initiator protein OS=Diporeia-associated CRESS-DNA virus LM122 OX=1974423 GN=Rep PE=4 SV=1
MM1 pKa = 7.28TQTQAYY7 pKa = 9.67SNWCFTAFKK16 pKa = 11.13DD17 pKa = 3.9MDD19 pKa = 3.75WPKK22 pKa = 11.44DD23 pKa = 3.7LIKK26 pKa = 9.72WDD28 pKa = 3.98KK29 pKa = 11.75YY30 pKa = 9.93EE31 pKa = 3.93LTYY34 pKa = 10.97LKK36 pKa = 10.19SQPEE40 pKa = 4.27KK41 pKa = 10.65CPKK44 pKa = 7.54TGKK47 pKa = 9.65YY48 pKa = 9.21HH49 pKa = 5.22IQGMIVVSPRR59 pKa = 11.84IRR61 pKa = 11.84LTALKK66 pKa = 10.18KK67 pKa = 9.8KK68 pKa = 8.64WPKK71 pKa = 9.81IHH73 pKa = 7.15LEE75 pKa = 4.02VTKK78 pKa = 10.48DD79 pKa = 3.37VQASVTYY86 pKa = 10.15CGKK89 pKa = 10.28DD90 pKa = 3.25DD91 pKa = 5.67SFEE94 pKa = 3.94AWDD97 pKa = 3.33GHH99 pKa = 5.13EE100 pKa = 4.55RR101 pKa = 11.84IEE103 pKa = 6.1LGTQPKK109 pKa = 10.36GQGQRR114 pKa = 11.84MDD116 pKa = 4.06LEE118 pKa = 4.16NLKK121 pKa = 9.82TILKK125 pKa = 9.94EE126 pKa = 3.65KK127 pKa = 10.64GGLKK131 pKa = 10.14RR132 pKa = 11.84IADD135 pKa = 3.96EE136 pKa = 5.16DD137 pKa = 3.84PASFLKK143 pKa = 10.76FSRR146 pKa = 11.84LEE148 pKa = 3.66KK149 pKa = 9.42WQQALQVDD157 pKa = 4.4PRR159 pKa = 11.84MKK161 pKa = 10.35EE162 pKa = 3.48PSEE165 pKa = 4.58RR166 pKa = 11.84EE167 pKa = 3.47FSVFFGPPGTGKK179 pKa = 10.36SRR181 pKa = 11.84IAYY184 pKa = 8.65EE185 pKa = 3.83MLGEE189 pKa = 4.12DD190 pKa = 4.28VYY192 pKa = 11.47IPVANNANKK201 pKa = 10.43LSFEE205 pKa = 4.46TYY207 pKa = 9.92NGEE210 pKa = 3.96GTILIDD216 pKa = 4.62DD217 pKa = 4.27FHH219 pKa = 7.22PEE221 pKa = 3.93HH222 pKa = 7.2LLGCC226 pKa = 4.67

Molecular weight: 26.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5SRU9|A0A1W5SRU9_9VIRU Putative replication initiator protein OS=Diporeia-associated CRESS-DNA virus LM122 OX=1974423 GN=Rep PE=4 SV=1
MM1 pKa = 7.11YY2 pKa = 10.49YY3 pKa = 10.25FRR5 pKa = 11.84RR6 pKa = 11.84WHH8 pKa = 6.14INKK11 pKa = 8.57QRR13 pKa = 11.84FSSQMRR19 pKa = 11.84YY20 pKa = 9.39ARR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 7.39PFRR27 pKa = 11.84GRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84VRR33 pKa = 11.84GRR35 pKa = 11.84RR36 pKa = 11.84VGRR39 pKa = 11.84RR40 pKa = 11.84SFKK43 pKa = 10.43RR44 pKa = 11.84RR45 pKa = 11.84TSIRR49 pKa = 11.84KK50 pKa = 8.86RR51 pKa = 11.84RR52 pKa = 11.84STSSSDD58 pKa = 2.92FVTIVKK64 pKa = 10.2RR65 pKa = 11.84IVIDD69 pKa = 3.52NQDD72 pKa = 2.83ILGGVNVVQEE82 pKa = 4.39FSTVIGDD89 pKa = 3.83VPDD92 pKa = 3.72VNSYY96 pKa = 10.34LALYY100 pKa = 7.82QQYY103 pKa = 10.16RR104 pKa = 11.84IRR106 pKa = 11.84KK107 pKa = 7.46ISFMQKK113 pKa = 9.3PNQNFAQIGGTIAKK127 pKa = 9.12PVFEE131 pKa = 4.79SIVMKK136 pKa = 9.58STYY139 pKa = 9.49YY140 pKa = 10.21PSIASLSEE148 pKa = 3.89VQNSSGYY155 pKa = 9.06KK156 pKa = 9.51RR157 pKa = 11.84GYY159 pKa = 9.24SLYY162 pKa = 10.2RR163 pKa = 11.84SWRR166 pKa = 11.84PLVDD170 pKa = 3.37IFTLNSLPPPNQAAANMMKK189 pKa = 10.25YY190 pKa = 10.41SPWLDD195 pKa = 3.24CRR197 pKa = 11.84TAQGTPANQPVHH209 pKa = 6.86HH210 pKa = 6.6GLAYY214 pKa = 10.16QSSLPPLFPGPHH226 pKa = 6.89LYY228 pKa = 10.55VIQQQVYY235 pKa = 8.56VVDD238 pKa = 4.52FRR240 pKa = 11.84YY241 pKa = 10.3KK242 pKa = 10.07RR243 pKa = 3.4

Molecular weight: 28.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

469

226

243

234.5

27.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.691 ± 0.125

1.066 ± 0.5

4.904 ± 1.231

4.478 ± 2.477

4.691 ± 0.189

6.183 ± 0.637

1.919 ± 0.208

5.757 ± 0.318

7.676 ± 2.091

6.823 ± 1.125

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.559 ± 0.068

3.625 ± 0.689

6.183 ± 0.306

5.97 ± 0.783

8.102 ± 2.927

7.463 ± 1.844

4.691 ± 0.754

6.183 ± 1.563

1.919 ± 0.523

5.117 ± 1.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski