Streptococcus phage SW30

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S5H247|A0A3S5H247_9CAUD Uncharacterized protein OS=Streptococcus phage SW30 OX=2419650 GN=SW30_027 PE=4 SV=1
MM1 pKa = 7.12VNWVDD6 pKa = 4.58KK7 pKa = 11.1DD8 pKa = 4.24GNDD11 pKa = 3.97LPDD14 pKa = 4.26GADD17 pKa = 3.29QEE19 pKa = 5.13FKK21 pKa = 11.28AGMFFSFAGDD31 pKa = 3.56EE32 pKa = 4.15NNITDD37 pKa = 3.82TGEE40 pKa = 3.65GGYY43 pKa = 10.61YY44 pKa = 9.76GGYY47 pKa = 8.0YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S5H256|A0A3S5H256_9CAUD Uncharacterized protein OS=Streptococcus phage SW30 OX=2419650 GN=SW30_040 PE=4 SV=1
MM1 pKa = 6.65TTLSKK6 pKa = 10.54RR7 pKa = 11.84DD8 pKa = 3.43NQLRR12 pKa = 11.84ILTAVINVTTIIRR25 pKa = 11.84VVNRR29 pKa = 11.84PIEE32 pKa = 4.27TVVIQAII39 pKa = 3.69

Molecular weight: 4.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

10207

39

1594

249.0

28.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.398 ± 0.508

0.568 ± 0.168

6.672 ± 0.244

6.456 ± 0.406

4.213 ± 0.228

6.672 ± 0.478

1.489 ± 0.13

6.701 ± 0.313

8.435 ± 0.513

7.799 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.177

6.574 ± 0.277

3.008 ± 0.201

4.144 ± 0.217

4.232 ± 0.291

6.29 ± 0.313

6.486 ± 0.291

6.035 ± 0.281

1.46 ± 0.167

4.017 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski