Gordonia phage Octobien14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3MAF3|A0A3G3MAF3_9CAUD Uncharacterized protein OS=Gordonia phage Octobien14 OX=2483673 GN=85 PE=4 SV=1
MM1 pKa = 7.75SDD3 pKa = 3.5LALSLLAEE11 pKa = 4.16SLIGKK16 pKa = 8.43RR17 pKa = 11.84VKK19 pKa = 10.52SATTNSLTLDD29 pKa = 4.05DD30 pKa = 4.23GTVIEE35 pKa = 4.81LFEE38 pKa = 4.44SASDD42 pKa = 4.07CCASAYY48 pKa = 10.43GDD50 pKa = 3.52WALADD55 pKa = 4.52AVDD58 pKa = 4.7LDD60 pKa = 4.13AAITAVDD67 pKa = 3.92LTSEE71 pKa = 4.07EE72 pKa = 4.47HH73 pKa = 6.58EE74 pKa = 5.62DD75 pKa = 3.76YY76 pKa = 10.91DD77 pKa = 3.76SHH79 pKa = 5.86YY80 pKa = 7.77TTGEE84 pKa = 3.52LRR86 pKa = 11.84IMHH89 pKa = 6.15NQNPIALGDD98 pKa = 4.06LYY100 pKa = 11.38ANSGNGGYY108 pKa = 9.77YY109 pKa = 10.43YY110 pKa = 10.82SVLSVKK116 pKa = 9.89VHH118 pKa = 6.52LPTGGSIQTEE128 pKa = 4.83LISSS132 pKa = 4.2

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3MAJ3|A0A3G3MAJ3_9CAUD Uncharacterized protein OS=Gordonia phage Octobien14 OX=2483673 GN=134 PE=4 SV=1
MM1 pKa = 7.59GKK3 pKa = 9.51HH4 pKa = 6.2KK5 pKa = 10.5GRR7 pKa = 11.84SGRR10 pKa = 11.84PYY12 pKa = 8.22RR13 pKa = 11.84TKK15 pKa = 10.19RR16 pKa = 11.84ANFRR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.04CAAEE26 pKa = 3.8NRR28 pKa = 11.84PCSLCGKK35 pKa = 8.97PIDD38 pKa = 3.84YY39 pKa = 9.12TLTGKK44 pKa = 9.89NPMAWTLEE52 pKa = 4.03HH53 pKa = 7.15IIPVSEE59 pKa = 5.22LKK61 pKa = 10.65RR62 pKa = 11.84IDD64 pKa = 3.57PNHH67 pKa = 6.86RR68 pKa = 11.84LLEE71 pKa = 4.36AVSNFAAAHH80 pKa = 5.76FLCNQRR86 pKa = 11.84KK87 pKa = 8.59QDD89 pKa = 2.76GGYY92 pKa = 9.46RR93 pKa = 11.84DD94 pKa = 4.2KK95 pKa = 11.08GTAGPAPAASSRR107 pKa = 11.84RR108 pKa = 11.84WTT110 pKa = 3.42

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

143

0

143

23896

24

2132

167.1

18.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.797 ± 0.447

1.042 ± 0.131

6.612 ± 0.182

6.394 ± 0.224

3.222 ± 0.162

8.278 ± 0.301

1.942 ± 0.132

4.176 ± 0.129

4.72 ± 0.194

8.386 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.091

3.268 ± 0.13

5.034 ± 0.203

3.365 ± 0.123

6.03 ± 0.184

5.867 ± 0.235

5.863 ± 0.199

7.545 ± 0.206

2.088 ± 0.129

2.959 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski