Bacillus phage Gp

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3S3L6|A0A7G3S3L6_9CAUD Uncharacterized protein OS=Bacillus phage Gp OX=2723960 GN=Gp_43 PE=4 SV=1
MM1 pKa = 6.98KK2 pKa = 10.22QGYY5 pKa = 8.58KK6 pKa = 10.45LNEE9 pKa = 3.98IDD11 pKa = 4.14EE12 pKa = 4.4MDD14 pKa = 2.97IGFYY18 pKa = 10.77FEE20 pKa = 5.21LVSNEE25 pKa = 3.96TNEE28 pKa = 3.93PEE30 pKa = 4.33VYY32 pKa = 9.61IDD34 pKa = 4.47QILL37 pKa = 3.45

Molecular weight: 4.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3WCV7|A0A7G3WCV7_9CAUD Phage_rep_O domain-containing protein OS=Bacillus phage Gp OX=2723960 GN=Gp_78 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.25NNYY5 pKa = 9.92RR6 pKa = 11.84IFEE9 pKa = 4.4NNVVIFLKK17 pKa = 10.5RR18 pKa = 11.84KK19 pKa = 9.02NGEE22 pKa = 3.7ILEE25 pKa = 4.47TVIDD29 pKa = 4.04LQDD32 pKa = 3.39FQKK35 pKa = 11.11ANEE38 pKa = 4.27YY39 pKa = 10.54KK40 pKa = 9.39GTWYY44 pKa = 10.81ALRR47 pKa = 11.84DD48 pKa = 3.73PKK50 pKa = 10.83SGKK53 pKa = 10.13FYY55 pKa = 11.01VYY57 pKa = 11.0GNLYY61 pKa = 9.23ISKK64 pKa = 10.1GKK66 pKa = 9.91RR67 pKa = 11.84RR68 pKa = 11.84GIKK71 pKa = 8.57LHH73 pKa = 6.07RR74 pKa = 11.84WLMGEE79 pKa = 3.72PCGFVIDD86 pKa = 5.33HH87 pKa = 6.99INHH90 pKa = 6.53DD91 pKa = 3.88TLDD94 pKa = 3.55NRR96 pKa = 11.84RR97 pKa = 11.84VNLRR101 pKa = 11.84AVKK104 pKa = 10.07QRR106 pKa = 11.84ANIQNSLKK114 pKa = 10.13PLKK117 pKa = 9.26TNKK120 pKa = 10.03SGFRR124 pKa = 11.84GVSWDD129 pKa = 3.28EE130 pKa = 4.31SSNKK134 pKa = 8.05WRR136 pKa = 11.84AQISINKK143 pKa = 9.21KK144 pKa = 9.35VINLGRR150 pKa = 11.84FSSKK154 pKa = 9.52QKK156 pKa = 10.57AHH158 pKa = 6.03AVYY161 pKa = 10.45LKK163 pKa = 10.75AKK165 pKa = 9.79KK166 pKa = 9.57KK167 pKa = 7.07YY168 pKa = 8.82HH169 pKa = 6.07KK170 pKa = 10.27EE171 pKa = 3.86GVISS175 pKa = 3.67

Molecular weight: 20.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

14148

29

1121

166.4

18.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.623 ± 0.513

1.159 ± 0.195

5.831 ± 0.226

8.757 ± 0.316

3.767 ± 0.201

5.838 ± 0.251

1.802 ± 0.146

7.379 ± 0.246

8.793 ± 0.389

8.355 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.721 ± 0.206

5.527 ± 0.219

2.94 ± 0.157

4.043 ± 0.198

4.425 ± 0.223

5.845 ± 0.227

5.457 ± 0.243

5.93 ± 0.24

1.159 ± 0.108

3.647 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski