Corynebacterium uterequi
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2149 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3HC83|A0A0G3HC83_9CORY UvrABC system protein B OS=Corynebacterium uterequi OX=1072256 GN=uvrB PE=3 SV=1
MM1 pKa = 6.8 TRR3 pKa = 11.84 IRR5 pKa = 11.84 ALAALGTVAAFALSGCSLLPSNDD28 pKa = 3.62 AGSDD32 pKa = 3.5 SSATDD37 pKa = 3.23 APAIEE42 pKa = 4.42 TPGAGSQGSAVSSAEE57 pKa = 4.01 SAPAKK62 pKa = 10.14 SDD64 pKa = 3.29 SAAAAPGTNYY74 pKa = 10.19 SDD76 pKa = 4.45 RR77 pKa = 11.84 IEE79 pKa = 4.21 ISSLPEE85 pKa = 3.21 NDD87 pKa = 3.76 IFITVQNLQLGGEE100 pKa = 4.46 CAAGEE105 pKa = 4.08 YY106 pKa = 10.19 TPGEE110 pKa = 4.25 KK111 pKa = 10.08 LDD113 pKa = 4.59 DD114 pKa = 3.85 LTGAQYY120 pKa = 10.17 VQLWVEE126 pKa = 4.2 QEE128 pKa = 3.91 AEE130 pKa = 4.34 RR131 pKa = 11.84 IDD133 pKa = 4.28 NPDD136 pKa = 3.32 SAPFATLYY144 pKa = 11.04 GPDD147 pKa = 3.53 YY148 pKa = 11.33 VDD150 pKa = 3.7 AQGQVLPAVAALDD163 pKa = 3.68 CRR165 pKa = 11.84 EE166 pKa = 3.89 VDD168 pKa = 5.1 GYY170 pKa = 10.59 EE171 pKa = 4.08 SWGKK175 pKa = 9.25 PVDD178 pKa = 4.1 PGTKK182 pKa = 8.26 AQHH185 pKa = 5.78 YY186 pKa = 8.59 GAFIIPEE193 pKa = 4.55 GATEE197 pKa = 3.9 IQIEE201 pKa = 4.43 GHH203 pKa = 5.69 SFPLL207 pKa = 4.03
Molecular weight: 21.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.681
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.592
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.164
Thurlkill 3.732
EMBOSS 3.783
Sillero 3.999
Patrickios 1.888
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A0G3HFV6|A0A0G3HFV6_9CORY Lycopene cyclase domain OS=Corynebacterium uterequi OX=1072256 GN=CUTER_00100 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 SRR17 pKa = 11.84 KK18 pKa = 7.88 HH19 pKa = 4.75 GFRR22 pKa = 11.84 IRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.72 KK40 pKa = 10.5 GRR42 pKa = 11.84 AKK44 pKa = 9.67 LTAA47 pKa = 4.21
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.749
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.486
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2149
0
2149
718476
39
2995
334.3
35.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.725 ± 0.08
0.697 ± 0.015
6.174 ± 0.051
5.86 ± 0.056
3.164 ± 0.033
8.494 ± 0.05
2.199 ± 0.026
4.738 ± 0.039
2.563 ± 0.042
9.776 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.101 ± 0.025
2.453 ± 0.026
5.24 ± 0.044
2.968 ± 0.026
6.691 ± 0.057
5.677 ± 0.038
6.186 ± 0.039
8.679 ± 0.048
1.355 ± 0.023
2.261 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here