Sweet potato C6 virus
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7GQ22|J7GQ22_9VIRU 7 kDa protein OS=Sweet potato C6 virus OX=1307958 PE=3 SV=1
MM1 pKa = 7.72 DD2 pKa = 5.14 VIVKK6 pKa = 10.18 KK7 pKa = 10.55 LRR9 pKa = 11.84 SRR11 pKa = 11.84 GFTFNKK17 pKa = 9.5 PIEE20 pKa = 4.37 GSFLVVNCVPGAGKK34 pKa = 10.39 SSLIRR39 pKa = 11.84 EE40 pKa = 4.71 LIEE43 pKa = 3.84 EE44 pKa = 4.3 DD45 pKa = 3.49 SRR47 pKa = 11.84 FEE49 pKa = 4.17 AFTFGHH55 pKa = 6.8 PDD57 pKa = 3.12 KK58 pKa = 11.22 PNVTNCFIKK67 pKa = 10.72 SSDD70 pKa = 3.96 EE71 pKa = 3.51 IRR73 pKa = 11.84 SRR75 pKa = 11.84 QFAIVDD81 pKa = 4.02 EE82 pKa = 4.36 YY83 pKa = 11.55 TEE85 pKa = 4.23 GDD87 pKa = 3.55 FRR89 pKa = 11.84 KK90 pKa = 9.48 FDD92 pKa = 4.93 PIAIFGDD99 pKa = 4.08 PCQSTAFKK107 pKa = 10.48 EE108 pKa = 4.01 PNLICNYY115 pKa = 9.69 FSTRR119 pKa = 11.84 TFRR122 pKa = 11.84 FGKK125 pKa = 8.62 ATAALLRR132 pKa = 11.84 SLGFNIWADD141 pKa = 3.47 KK142 pKa = 10.73 EE143 pKa = 4.29 DD144 pKa = 3.85 TVEE147 pKa = 3.77 IGHH150 pKa = 5.99 IFEE153 pKa = 5.38 RR154 pKa = 11.84 EE155 pKa = 3.76 PEE157 pKa = 4.23 GQIVCYY163 pKa = 9.86 EE164 pKa = 4.04 PEE166 pKa = 3.67 IQRR169 pKa = 11.84 LVEE172 pKa = 4.02 RR173 pKa = 11.84 HH174 pKa = 5.98 SGFALTVEE182 pKa = 5.27 DD183 pKa = 3.69 IRR185 pKa = 11.84 GITEE189 pKa = 4.04 DD190 pKa = 3.56 VVTFITSEE198 pKa = 4.05 TKK200 pKa = 10.33 FDD202 pKa = 3.97 NKK204 pKa = 10.12 HH205 pKa = 5.37 SALIYY210 pKa = 10.32 LCLTRR215 pKa = 11.84 HH216 pKa = 5.01 MSKK219 pKa = 10.82 LLILTPDD226 pKa = 3.33 GSYY229 pKa = 10.6 TSSS232 pKa = 2.95
Molecular weight: 26.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.32
IPC2_protein 5.385
IPC_protein 5.321
Toseland 5.385
ProMoST 5.512
Dawson 5.385
Bjellqvist 5.474
Wikipedia 5.283
Rodwell 5.321
Grimsley 5.359
Solomon 5.385
Lehninger 5.347
Nozaki 5.537
DTASelect 5.69
Thurlkill 5.461
EMBOSS 5.41
Sillero 5.626
Patrickios 4.228
IPC_peptide 5.397
IPC2_peptide 5.626
IPC2.peptide.svr19 5.639
Protein with the highest isoelectric point:
>tr|J7GU58|J7GU58_9VIRU Movement protein TGB2 OS=Sweet potato C6 virus OX=1307958 PE=3 SV=1
MM1 pKa = 7.74 ALTPPPDD8 pKa = 3.34 HH9 pKa = 7.12 SKK11 pKa = 11.08 SLLAICVGIAIALVLYY27 pKa = 10.78 SLTRR31 pKa = 11.84 SNLPHH36 pKa = 7.58 VGDD39 pKa = 4.21 NLHH42 pKa = 6.4 SLPHH46 pKa = 6.2 GGCYY50 pKa = 9.21 QDD52 pKa = 3.43 GTKK55 pKa = 10.35 SISYY59 pKa = 9.36 NSPARR64 pKa = 11.84 TYY66 pKa = 9.92 PASTLLAKK74 pKa = 10.39 SAFNPFLIVLLLSACIFILSKK95 pKa = 10.75 NQSHH99 pKa = 6.15 SCRR102 pKa = 11.84 SCRR105 pKa = 11.84 GYY107 pKa = 10.99 CC108 pKa = 3.95
Molecular weight: 11.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.057
IPC2_protein 8.097
IPC_protein 8.053
Toseland 7.629
ProMoST 8.521
Dawson 8.624
Bjellqvist 9.033
Wikipedia 8.551
Rodwell 8.639
Grimsley 7.541
Solomon 8.799
Lehninger 8.814
Nozaki 9.253
DTASelect 8.726
Thurlkill 8.756
EMBOSS 8.829
Sillero 9.107
Patrickios 0.846
IPC_peptide 8.785
IPC2_peptide 8.507
IPC2.peptide.svr19 8.453
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2724
78
2001
544.8
61.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.874 ± 0.987
2.019 ± 0.706
5.91 ± 0.53
6.241 ± 0.727
6.424 ± 0.921
5.103 ± 0.428
2.239 ± 0.286
6.131 ± 0.622
7.416 ± 0.949
11.087 ± 1.865
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.762 ± 0.269
5.029 ± 0.495
3.965 ± 0.387
3.084 ± 0.445
5.029 ± 0.432
9.398 ± 0.659
4.626 ± 0.659
4.919 ± 0.346
0.954 ± 0.312
2.753 ± 0.41
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here