Synechococcus phage S-LBS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I5ARA3|A0A2I5ARA3_9CAUD Uncharacterized protein OS=Synechococcus phage S-LBS1 OX=2008320 GN=SLBS1_A07 PE=4 SV=1
MM1 pKa = 7.78AFTEE5 pKa = 4.07NLDD8 pKa = 3.34VFLADD13 pKa = 4.39FGVSVTAGAVSGLGLLDD30 pKa = 3.54MPGEE34 pKa = 4.13LVADD38 pKa = 4.09GMVISTDD45 pKa = 3.31YY46 pKa = 10.84MLTAKK51 pKa = 10.59ADD53 pKa = 3.84DD54 pKa = 4.55FGSLLYY60 pKa = 10.78GDD62 pKa = 5.18SILVNSDD69 pKa = 3.95LYY71 pKa = 10.89SVRR74 pKa = 11.84QVLLIDD80 pKa = 4.47DD81 pKa = 4.08GRR83 pKa = 11.84MIQAGLQLVTAAAQAVYY100 pKa = 10.73VPDD103 pKa = 3.64VFQAGVYY110 pKa = 9.45LL111 pKa = 4.12

Molecular weight: 11.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I5ARE3|A0A2I5ARE3_9CAUD Superfamily II DNA or RNA helicase OS=Synechococcus phage S-LBS1 OX=2008320 GN=SLBS1_A50 PE=4 SV=1
MM1 pKa = 6.6TTPPRR6 pKa = 11.84WTQPEE11 pKa = 4.48LDD13 pKa = 4.26HH14 pKa = 7.34LEE16 pKa = 4.25SLAGDD21 pKa = 3.8VPFPTLLRR29 pKa = 11.84SMHH32 pKa = 5.88YY33 pKa = 10.21KK34 pKa = 9.14ATAEE38 pKa = 4.0GWPPRR43 pKa = 11.84TDD45 pKa = 2.97KK46 pKa = 11.31AIVMRR51 pKa = 11.84MRR53 pKa = 11.84RR54 pKa = 11.84SRR56 pKa = 11.84QFCRR60 pKa = 11.84ARR62 pKa = 11.84TGEE65 pKa = 3.72WTTTYY70 pKa = 10.95GAGEE74 pKa = 4.01ILGCPGTRR82 pKa = 11.84VEE84 pKa = 3.51AWLRR88 pKa = 11.84RR89 pKa = 11.84KK90 pKa = 9.83PIARR94 pKa = 11.84ILDD97 pKa = 3.75PQTTGGTRR105 pKa = 11.84YY106 pKa = 9.57INRR109 pKa = 11.84RR110 pKa = 11.84SWRR113 pKa = 11.84RR114 pKa = 11.84LAKK117 pKa = 10.07QMPRR121 pKa = 11.84VLGGFSSDD129 pKa = 3.52ALFLLLEE136 pKa = 4.77DD137 pKa = 5.02RR138 pKa = 11.84DD139 pKa = 3.96LADD142 pKa = 6.35AIAQQHH148 pKa = 6.02PRR150 pKa = 11.84PIGDD154 pKa = 2.72WRR156 pKa = 11.84VRR158 pKa = 11.84CIEE161 pKa = 3.77TGEE164 pKa = 4.41IYY166 pKa = 10.64SSCGAAARR174 pKa = 11.84EE175 pKa = 3.96LHH177 pKa = 5.52VTQACISLAIRR188 pKa = 11.84QARR191 pKa = 11.84PVAALGMTFEE201 pKa = 4.74ALRR204 pKa = 11.84GVGPVLGG211 pKa = 4.21

Molecular weight: 23.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11114

37

740

213.7

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.931 ± 0.49

1.242 ± 0.184

5.255 ± 0.248

5.543 ± 0.405

3.068 ± 0.208

8.251 ± 0.444

1.665 ± 0.186

5.192 ± 0.233

3.14 ± 0.247

8.827 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.158

3.059 ± 0.268

5.102 ± 0.242

4.634 ± 0.255

6.478 ± 0.487

6.676 ± 0.403

7.018 ± 0.509

6.793 ± 0.239

1.728 ± 0.18

2.276 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski