Klebsiella phage KN4-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; Klebsiella virus KN4-1

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S5IBI0|A0A3S5IBI0_9CAUD Single-stranded DNA-binding protein OS=Klebsiella phage KN4-1 OX=2282631 PE=4 SV=1
MM1 pKa = 7.38NMQDD5 pKa = 4.11AYY7 pKa = 10.62FGSAAEE13 pKa = 3.97LDD15 pKa = 4.2AVNEE19 pKa = 3.86MLAAIGEE26 pKa = 4.7SPVTTLDD33 pKa = 3.33EE34 pKa = 5.18DD35 pKa = 4.25GSADD39 pKa = 3.27VANARR44 pKa = 11.84RR45 pKa = 11.84ILNRR49 pKa = 11.84INRR52 pKa = 11.84QIQSKK57 pKa = 9.61GWAFNINEE65 pKa = 4.55SATLTPDD72 pKa = 2.99ASTGLIPFRR81 pKa = 11.84PAYY84 pKa = 10.44LSILGGQYY92 pKa = 10.17VNRR95 pKa = 11.84GGWVYY100 pKa = 11.2DD101 pKa = 3.54KK102 pKa = 11.07STGTDD107 pKa = 3.14TFSGPITVTMITLQDD122 pKa = 3.63YY123 pKa = 11.57DD124 pKa = 4.19EE125 pKa = 4.72MPEE128 pKa = 5.08CFRR131 pKa = 11.84QWIVTKK137 pKa = 10.66ASRR140 pKa = 11.84QFNSRR145 pKa = 11.84FFGAEE150 pKa = 3.73DD151 pKa = 3.76VEE153 pKa = 4.46NSLAQEE159 pKa = 3.88EE160 pKa = 4.75MEE162 pKa = 5.66ARR164 pKa = 11.84MACNEE169 pKa = 3.84YY170 pKa = 10.91EE171 pKa = 4.14MDD173 pKa = 3.81FGQYY177 pKa = 11.27NMIDD181 pKa = 3.29GDD183 pKa = 4.37SFVGGIIGRR192 pKa = 3.91

Molecular weight: 21.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9WWB4|A0A3Q9WWB4_9CAUD Capsid and scaffold protein OS=Klebsiella phage KN4-1 OX=2282631 PE=4 SV=1
MM1 pKa = 7.79AIALLVEE8 pKa = 4.48SSGVNHH14 pKa = 5.91QPHH17 pKa = 6.94KK18 pKa = 9.79EE19 pKa = 3.64GQMANKK25 pKa = 9.73RR26 pKa = 11.84KK27 pKa = 9.64PIVNGEE33 pKa = 4.28KK34 pKa = 10.12EE35 pKa = 4.13CSKK38 pKa = 10.52CSEE41 pKa = 4.07WKK43 pKa = 9.95PLSEE47 pKa = 4.29YY48 pKa = 10.39GKK50 pKa = 10.62SSGRR54 pKa = 11.84PSGQSQCKK62 pKa = 8.28EE63 pKa = 3.74CRR65 pKa = 11.84KK66 pKa = 9.53SGKK69 pKa = 9.5RR70 pKa = 11.84GKK72 pKa = 7.9PTSAQCRR79 pKa = 11.84SSHH82 pKa = 6.61RR83 pKa = 11.84EE84 pKa = 3.62WILKK88 pKa = 7.13NTYY91 pKa = 10.47GITSEE96 pKa = 4.0EE97 pKa = 4.2FGRR100 pKa = 11.84MLEE103 pKa = 4.25EE104 pKa = 3.59QGGVCRR110 pKa = 11.84ICHH113 pKa = 6.47KK114 pKa = 10.65GPSGRR119 pKa = 11.84FKK121 pKa = 10.69HH122 pKa = 6.21LCVDD126 pKa = 3.88HH127 pKa = 6.79CHH129 pKa = 5.24TTGKK133 pKa = 9.91VRR135 pKa = 11.84GLLCHH140 pKa = 6.98ACNKK144 pKa = 10.39SLGFLEE150 pKa = 5.6DD151 pKa = 3.34DD152 pKa = 3.64PARR155 pKa = 11.84IKK157 pKa = 10.83RR158 pKa = 11.84LIDD161 pKa = 3.38YY162 pKa = 10.44LGGPNEE168 pKa = 4.11PSKK171 pKa = 11.2SVNKK175 pKa = 10.36KK176 pKa = 8.43SS177 pKa = 3.25

Molecular weight: 19.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

9164

69

1330

458.2

50.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.872 ± 0.572

1.091 ± 0.235

6.427 ± 0.211

6.918 ± 0.55

3.514 ± 0.178

8.468 ± 0.503

1.997 ± 0.296

4.943 ± 0.261

6.198 ± 0.495

8.326 ± 0.473

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.171

4.256 ± 0.213

3.907 ± 0.166

4.114 ± 0.244

5.282 ± 0.233

6.176 ± 0.551

5.227 ± 0.302

6.973 ± 0.415

1.353 ± 0.247

3.416 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski