Streptococcus satellite phage Javan614
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZSJ8|A0A4D5ZSJ8_9VIRU DNA primase OS=Streptococcus satellite phage Javan614 OX=2558784 GN=JavanS614_0009 PE=4 SV=1
MM1 pKa = 7.16 STTEE5 pKa = 3.86 KK6 pKa = 10.58 LRR8 pKa = 11.84 EE9 pKa = 3.69 FGINRR14 pKa = 11.84 ICLSPYY20 pKa = 9.29 KK21 pKa = 10.0 KK22 pKa = 10.36 YY23 pKa = 10.8 SRR25 pKa = 11.84 GYY27 pKa = 9.78 SEE29 pKa = 5.23 PGNIGTGYY37 pKa = 10.11 VAGLKK42 pKa = 9.94 VDD44 pKa = 3.2 AGVRR48 pKa = 11.84 DD49 pKa = 3.82 KK50 pKa = 11.39 TDD52 pKa = 3.19 DD53 pKa = 3.57 TVLDD57 pKa = 4.96 GIVSYY62 pKa = 11.25 DD63 pKa = 3.13 RR64 pKa = 11.84 AEE66 pKa = 4.21 AKK68 pKa = 9.73 NAYY71 pKa = 8.97 IGQINMEE78 pKa = 4.36 TASSFTGLQGVTLGYY93 pKa = 10.74 DD94 pKa = 3.23 ILRR97 pKa = 11.84 NPAVDD102 pKa = 3.43 EE103 pKa = 4.25 EE104 pKa = 4.71 KK105 pKa = 10.72 PLFVEE110 pKa = 4.5 PQWDD114 pKa = 4.03 GSEE117 pKa = 4.14 LPIYY121 pKa = 9.44 DD122 pKa = 4.78 AKK124 pKa = 10.64 PLQDD128 pKa = 3.72 ALVEE132 pKa = 4.21 YY133 pKa = 10.42 FGTEE137 pKa = 3.1 SDD139 pKa = 3.35 RR140 pKa = 11.84 RR141 pKa = 11.84 HH142 pKa = 5.76 NLVPGAMVVCANKK155 pKa = 10.39 GVTAYY160 pKa = 10.28 RR161 pKa = 11.84 PQEE164 pKa = 4.14 DD165 pKa = 4.2 RR166 pKa = 11.84 PFQAGEE172 pKa = 4.49 GYY174 pKa = 10.43 GVWSAIAISFAADD187 pKa = 3.29 GTVNSDD193 pKa = 3.62 MFIEE197 pKa = 5.0 DD198 pKa = 3.05 AGVWTKK204 pKa = 11.27 NDD206 pKa = 3.28 NEE208 pKa = 4.26 ADD210 pKa = 4.37 LIAWLDD216 pKa = 3.63 TRR218 pKa = 11.84 RR219 pKa = 11.84 KK220 pKa = 10.15 AIAKK224 pKa = 9.97 SIAEE228 pKa = 4.54 CGQDD232 pKa = 3.07 SHH234 pKa = 7.59 VRR236 pKa = 11.84 FARR239 pKa = 11.84 TYY241 pKa = 9.98 IGFAHH246 pKa = 7.27 AMMKK250 pKa = 10.16 PNQIGNAITVGPYY263 pKa = 9.57 FAIPVDD269 pKa = 5.17 AIPGGDD275 pKa = 2.97 ITDD278 pKa = 4.12 PDD280 pKa = 3.99 GCFDD284 pKa = 3.72 ILEE287 pKa = 4.6 HH288 pKa = 7.1 LSLPDD293 pKa = 3.1 WLDD296 pKa = 3.05 AMNYY300 pKa = 8.61 TSLTANDD307 pKa = 3.71 QIKK310 pKa = 9.97 YY311 pKa = 10.21
Molecular weight: 34.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.494
IPC2_protein 4.52
IPC_protein 4.482
Toseland 4.304
ProMoST 4.622
Dawson 4.457
Bjellqvist 4.609
Wikipedia 4.368
Rodwell 4.317
Grimsley 4.215
Solomon 4.457
Lehninger 4.406
Nozaki 4.558
DTASelect 4.787
Thurlkill 4.329
EMBOSS 4.38
Sillero 4.609
Patrickios 3.643
IPC_peptide 4.457
IPC2_peptide 4.596
IPC2.peptide.svr19 4.535
Protein with the highest isoelectric point:
>tr|A0A4D5ZT10|A0A4D5ZT10_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan614 OX=2558784 GN=JavanS614_0011 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.26 INEE5 pKa = 4.22 IKK7 pKa = 10.67 KK8 pKa = 9.76 KK9 pKa = 10.54 DD10 pKa = 3.3 GSTVYY15 pKa = 10.36 RR16 pKa = 11.84 ANIYY20 pKa = 10.62 LGVDD24 pKa = 3.95 VITGKK29 pKa = 10.74 KK30 pKa = 7.55 VTTKK34 pKa = 9.32 VTARR38 pKa = 11.84 TKK40 pKa = 10.91 KK41 pKa = 9.48 EE42 pKa = 4.24 LKK44 pKa = 9.67 TKK46 pKa = 10.1 AQQAQFDD53 pKa = 4.54 FKK55 pKa = 11.59 ANGSTRR61 pKa = 11.84 YY62 pKa = 9.72 KK63 pKa = 9.99 EE64 pKa = 4.21 VAIEE68 pKa = 4.25 TYY70 pKa = 10.63 EE71 pKa = 3.95 EE72 pKa = 4.45 LAISWWDD79 pKa = 3.38 SYY81 pKa = 11.84 KK82 pKa = 10.19 NTVKK86 pKa = 10.93 ANTRR90 pKa = 11.84 NTQKK94 pKa = 11.26 GLLNNHH100 pKa = 5.72 VLPLFGEE107 pKa = 4.67 FKK109 pKa = 10.56 LDD111 pKa = 3.59 KK112 pKa = 10.06 LTTPLIQSTMNKK124 pKa = 9.46 LANSTNTGEE133 pKa = 3.98 ASAYY137 pKa = 8.99 LHH139 pKa = 6.25 YY140 pKa = 11.02 GKK142 pKa = 9.53 IHH144 pKa = 5.96 TLNKK148 pKa = 10.19 RR149 pKa = 11.84 ILQYY153 pKa = 11.17 GVILQVIPTNPANNVVLPRR172 pKa = 11.84 NTQKK176 pKa = 11.11 DD177 pKa = 3.42 KK178 pKa = 10.95 KK179 pKa = 10.82 DD180 pKa = 3.45 KK181 pKa = 10.98 VKK183 pKa = 10.8 HH184 pKa = 5.54 FNNDD188 pKa = 2.8 EE189 pKa = 4.08 LKK191 pKa = 10.82 QFLTYY196 pKa = 10.53 LDD198 pKa = 4.07 SLDD201 pKa = 3.36 NSKK204 pKa = 11.22 YY205 pKa = 8.53 KK206 pKa = 10.13 TYY208 pKa = 11.4 YY209 pKa = 10.78 DD210 pKa = 2.93 ITLYY214 pKa = 10.96 KK215 pKa = 10.3 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 4.0 ALALSWSDD234 pKa = 2.86 IDD236 pKa = 4.49 LDD238 pKa = 3.79 NSVVHH243 pKa = 5.55 ITKK246 pKa = 9.12 TLNCDD251 pKa = 3.19 MKK253 pKa = 11.32 LNSPKK258 pKa = 10.63 SKK260 pKa = 10.55 AGYY263 pKa = 9.68 RR264 pKa = 11.84 DD265 pKa = 3.11 IDD267 pKa = 3.33 IDD269 pKa = 3.84 QQTVTMLKK277 pKa = 10.09 KK278 pKa = 9.84 YY279 pKa = 8.29 QRR281 pKa = 11.84 KK282 pKa = 6.06 QTQEE286 pKa = 3.04 AWKK289 pKa = 10.12 LGRR292 pKa = 11.84 TEE294 pKa = 4.07 TVVFSDD300 pKa = 5.91 FINAYY305 pKa = 9.33 PNYY308 pKa = 9.28 KK309 pKa = 10.23 ALAGRR314 pKa = 11.84 LRR316 pKa = 11.84 SYY318 pKa = 10.28 FKK320 pKa = 10.67 RR321 pKa = 11.84 AGVNNIGFHH330 pKa = 5.79 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.0 KK348 pKa = 10.34 EE349 pKa = 3.8 LQHH352 pKa = 6.73 RR353 pKa = 11.84 LGHH356 pKa = 5.68 STLSMTMDD364 pKa = 4.17 TYY366 pKa = 11.8 SHH368 pKa = 7.15 LSKK371 pKa = 11.01 EE372 pKa = 4.3 NAKK375 pKa = 10.31 KK376 pKa = 9.35 ATSFYY381 pKa = 10.1 EE382 pKa = 3.9 QALKK386 pKa = 10.8 SII388 pKa = 4.54
Molecular weight: 44.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.042
IPC2_protein 9.18
IPC_protein 9.048
Toseland 9.838
ProMoST 9.516
Dawson 10.058
Bjellqvist 9.706
Wikipedia 10.218
Rodwell 10.555
Grimsley 10.131
Solomon 10.072
Lehninger 10.028
Nozaki 9.823
DTASelect 9.706
Thurlkill 9.897
EMBOSS 10.248
Sillero 9.955
Patrickios 9.736
IPC_peptide 10.072
IPC2_peptide 8.17
IPC2.peptide.svr19 8.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
3220
61
502
189.4
21.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.894 ± 0.659
0.559 ± 0.122
5.776 ± 0.658
6.211 ± 0.834
4.255 ± 0.419
6.242 ± 0.51
1.522 ± 0.279
6.863 ± 0.469
8.323 ± 0.858
9.41 ± 0.708
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.516 ± 0.364
5.621 ± 0.572
3.975 ± 0.5
3.82 ± 0.537
3.602 ± 0.367
5.963 ± 0.518
6.739 ± 0.616
5.901 ± 0.338
1.242 ± 0.195
4.565 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here