Sinimarinibacterium sp. NLF-5-8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Nevskiales; Sinobacteraceae; Sinimarinibacterium; unclassified Sinimarinibacterium

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P1U8A5|A0A6P1U8A5_9GAMM Cellulose synthase catalytic subunit [UDP-forming] OS=Sinimarinibacterium sp. NLF-5-8 OX=2698684 GN=bcsA PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.23KK3 pKa = 9.41WICVICGFVYY13 pKa = 10.7DD14 pKa = 4.79EE15 pKa = 4.51AAGLPDD21 pKa = 4.58HH22 pKa = 7.55DD23 pKa = 4.54IAPGTRR29 pKa = 11.84FEE31 pKa = 6.13DD32 pKa = 4.54IPDD35 pKa = 3.38DD36 pKa = 3.93WMCPEE41 pKa = 4.32CASPKK46 pKa = 10.47SSFEE50 pKa = 4.25PYY52 pKa = 10.45EE53 pKa = 3.92EE54 pKa = 4.1

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P1U9X9|A0A6P1U9X9_9GAMM Phospholipase C OS=Sinimarinibacterium sp. NLF-5-8 OX=2698684 GN=GT972_10125 PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.58QPKK9 pKa = 9.34KK10 pKa = 9.24LRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 7.83RR15 pKa = 11.84THH17 pKa = 5.96GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.27GGRR29 pKa = 11.84AVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.93GRR40 pKa = 11.84KK41 pKa = 8.85RR42 pKa = 11.84LSAA45 pKa = 3.96

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2927

0

2927

959076

24

2986

327.7

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.445 ± 0.057

0.98 ± 0.017

5.611 ± 0.034

5.072 ± 0.039

3.421 ± 0.03

7.888 ± 0.044

2.384 ± 0.022

4.891 ± 0.033

3.07 ± 0.036

10.751 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.022

2.851 ± 0.029

5.127 ± 0.029

4.803 ± 0.036

6.969 ± 0.047

5.273 ± 0.035

5.178 ± 0.032

7.081 ± 0.04

1.487 ± 0.02

2.427 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski