Microviridae sp. ctjWc39

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W842|A0A5Q2W842_9VIRU DNA binding protein OS=Microviridae sp. ctjWc39 OX=2656702 PE=4 SV=1
MM1 pKa = 7.42LLKK4 pKa = 10.81VFTIYY9 pKa = 10.55DD10 pKa = 4.01SKK12 pKa = 11.76AEE14 pKa = 4.3AYY16 pKa = 8.36LQPFFLKK23 pKa = 10.84NKK25 pKa = 9.63GEE27 pKa = 4.6AIRR30 pKa = 11.84ALTDD34 pKa = 3.29IVAKK38 pKa = 9.9PDD40 pKa = 3.62HH41 pKa = 6.59NFCKK45 pKa = 10.51YY46 pKa = 10.47PEE48 pKa = 4.71DD49 pKa = 3.57FTLFEE54 pKa = 5.46LGVFDD59 pKa = 5.99DD60 pKa = 3.8STSRR64 pKa = 11.84FEE66 pKa = 5.99LYY68 pKa = 7.8DD69 pKa = 3.74TPHH72 pKa = 6.02SVCVLIEE79 pKa = 4.32LKK81 pKa = 10.82RR82 pKa = 11.84SVTPSAA88 pKa = 3.96

Molecular weight: 10.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W7V5|A0A5Q2W7V5_9VIRU Scaffold protein OS=Microviridae sp. ctjWc39 OX=2656702 PE=4 SV=1
MM1 pKa = 7.27VKK3 pKa = 10.4GIPARR8 pKa = 11.84IPLIFINWVVKK19 pKa = 9.86VLIVYY24 pKa = 10.74AMLSSFASLLFRR36 pKa = 11.84AFRR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 9.62EE42 pKa = 3.62GFAFFIRR49 pKa = 11.84AYY51 pKa = 8.71EE52 pKa = 3.74ICFAA56 pKa = 4.97

Molecular weight: 6.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1449

56

556

207.0

23.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.798 ± 1.08

1.794 ± 0.5

6.556 ± 0.667

4.141 ± 0.443

6.073 ± 0.609

6.763 ± 0.521

2.001 ± 0.431

3.727 ± 0.556

6.142 ± 1.315

9.386 ± 0.505

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.298

4.555 ± 0.671

5.176 ± 0.764

3.244 ± 0.456

5.935 ± 0.795

7.867 ± 0.629

5.59 ± 0.852

5.452 ± 0.499

1.173 ± 0.231

4.072 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski